Reviewed, UniProtKB/Swiss-Prot A7TF84 (ATG26_VANPO)

Names and origin

Protein names

Recommended name:
    Sterol 3-beta-glucosyltransferase
    EC=2.4.1.173
Alternative name(s):
    Autophagy-related protein 26

Gene names

Name:	ATG26
ORF Names:	Kpol_2000p77

Organism

Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]

Taxonomic identifier

436907 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Vanderwaltozyma

Protein attributes

Sequence length	1217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Cytoplasm Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1217	1217	Sterol 3-beta-glucosyltransferase		PRO_0000318049
Regions
	Domain	195 – 232	38	GRAM 1
	Domain	246 – 343	98	PH
	Domain	590 – 656	67	GRAM 2

Sequences

	Sequence		Length	Mass (Da)
	A7TF84-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 57C553A3F01C3653	FASTA	1,217	138,412
	10 20 30 40 50 60 MIRNSTNTNN IMETEVALKD WILYYHKLCE VNSGEIFSGN EIITDFDDPV LSDPLLNLKP 70 80 90 100 110 120 LPKNEVTVNK DNVIKDNDYS SNGCDFAKSK FHIKGLVGLL TTASVYIGLD NSTNPSYDIS 130 140 150 160 170 180 QACEFDLEDN TVQQGTGRSA NMNPACKVAG FNKSSVRDTI FNIFKIPKKD EKISNDMEVN 190 200 210 220 230 240 NTLSRDLDYK KDLFEFVRKY FGISEEETLI GHYTGWLLQE VLIQGNLFIT NSSLVYLAHL 250 260 270 280 290 300 PKLTDAVVLC GKLKLRSRLK GNPRYWCVLK HSTLALYNDP SDIYFPILSI DLNHVEEISL 310 320 330 340 350 360 ENSLKDENTM TFVLEASSKN YKFIAGSSHS AKTWVKCLKK QLFSIKNQSK DTIGIKFPIS 370 380 390 400 410 420 SIIDVECQSY MNQGQSIKVK CFDGNKNYAL KEYTFLFFDK DGDSFYNIIS NFIPRNMNPV 430 440 450 460 470 480 EASLQNSNPT IHLNSKSHFN DKIKILSAVN IDDIICDTDS FEMTSSDILS DIDENIQPLS 490 500 510 520 530 540 LKHVRDISNC KNYLKEDLKT SNKMTIQDCN KKVQTSEIQL ITYSKHDSRM ELNDDSKYYQ 550 560 570 580 590 600 GNIGNDNKGI LSFKNICSIW NTSPIHNKDS DNFFMTSDPF VTSINDTTLA KIKDWFNLHD 610 620 630 640 650 660 NEVLHALYYA YLIKGYPVYG KLYVTNRRLY FKSCIPGVNV KMVLPLEDIE GYNEILGTNY 670 680 690 700 710 720 GNFGILLTVQ NEKELQFGFN SCTNRSDFEN VLQRCLDICK YAIKTPELVT SRVIESESEH 730 740 750 760 770 780 SRLRFFEEKF STEGIDIPFL VEDNPYFKTK IMPTKSYNFG FLTIGSRGDV QPYIALAKGL 790 800 810 820 830 840 IQEGHSVTII THREFKSFVE CHGIDFKEIA GDPTKLMSLM VEHEAINVGM LMEASSKFRG 850 860 870 880 890 900 WIHDLLVTTW EACKNLKLDI LIESPSAMAG IHISEALQIP YFRAFTMPWT RTRAYPHAFI 910 920 930 940 950 960 VPDQKRGGSF NYLTHVIFEN VFWRGICSQV NKWRVQTLGL EKTNLAQLQQ NKIPFLYNIS 970 980 990 1000 1010 1020 PVIFPPAIDF DEWIKVTGYW FLDESESFEP SQELETFISK ARKLGKKLVY IGFGSIVVNN 1030 1040 1050 1060 1070 1080 AKEMTRAVID SVLETDIFCI LNKGWSERLG KEELRYEEEP EYPETIFLCD SIPHDWLFPK 1090 1100 1110 1120 1130 1140 VDAAVHHGGS GTTGATLKAG TPVVIKPFFG DQFFFASRIE DIGAGIALKK LNVSSLSNAI 1150 1160 1170 1180 1190 1200 KKVLTDKSIK RKAVSLKKRV AKENGVTTAI NCIYSELEYA RSLVVKKNHK SSNIEFIQHP 1210 NNVNDTTKTV IPLTSMV

References

[1]

"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication." Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H. Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed: 17494770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS480382 Genomic DNA. Translation: EDO19109.1.
RefSeq	XP_001646967.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5547438.
KEGG	vpo:Kpol_2000p77.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002213. UDP_glucos_trans. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. PF00201. UDPGT. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_VANPO

Accession

Primary (citable) accession number: A7TF84

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	October 13, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)