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Protein

Lipoyl synthase, mitochondrial

Gene

v1g225637

Organism
Nematostella vectensis (Starlet sea anemone)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Putative lipoyltransferase 2, mitochondrial (v1g139271)
  2. Lipoyl synthase, mitochondrial (v1g225637)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi50Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi56Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi71Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi75Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi78Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:v1g225637
OrganismiNematostella vectensis (Starlet sea anemone)
Taxonomic identifieri45351 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaAnthozoaHexacoralliaActiniariaEdwardsiidaeNematostella
Proteomesi
  • UP000001593 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003982261 – 291Lipoyl synthase, mitochondrialAdd BLAST291

Interactioni

Protein-protein interaction databases

STRINGi45351.NEMVEDRAFT_v1g225637-PA

Structurei

3D structure databases

ProteinModelPortaliA7TDI2
SMRiA7TDI2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
HOGENOMiHOG000235998
InParanoidiA7TDI2
KOiK03644
OMAiWIRAKIS
OrthoDBiEOG091G0AXJ

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

A7TDI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTVTENVFP PREPKPKWLR VKLPTGKNYT ELRGLVDKYK LNTICASGSC
60 70 80 90 100
PNMGECWGEG TATFMILGNI CTRSCGFCGV KTGRPETVDW DEPEKVARSI
110 120 130 140 150
KLMKIKHAVI TSVDRDDLKD MGSIIWAETV KAIRRMNPNT TLETLIPDFQ
160 170 180 190 200
GNTRNLDRII EVAPEVVSHN METVKRLTRE VRIQAKYEKS LEVLRYLKAQ
210 220 230 240 250
GIKRTKSGIM LGLGEKEEEV IQVLHDLRDA NVDIVTIGQY LQPSKKHLPV
260 270 280 290
KEYISPEQFE KYEKIGKELG FRHVESGALV RSSYHAEKHI H
Length:291
Mass (Da):33,132
Last modified:October 2, 2007 - v1
Checksum:i974657A99F1109DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS477892 Genomic DNA Translation: EDO25859.1
RefSeqiXP_001617959.1, XM_001617909.1

Genome annotation databases

EnsemblMetazoaiEDO25859; EDO25859; NEMVEDRAFT_v1g225637
GeneIDi5496152
KEGGinve:NEMVE_v1g225637

Similar proteinsi

Entry informationi

Entry nameiLIAS_NEMVE
AccessioniPrimary (citable) accession number: A7TDI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 2, 2007
Last modified: May 23, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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