Reviewed, UniProtKB/Swiss-Prot A7T395 (SPAST_NEMVE)

Names and origin

Protein names

Recommended name:
    Spastin
    EC=3.6.4.3

Gene names

ORF Names:

v1g144095

Organism

Nematostella vectensis (Starlet sea anemone) [Complete proteome]

Taxonomic identifier

45351 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Cnidaria › Anthozoa › Hexacorallia › Actiniaria › Edwardsiidae › Nematostella

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the microtubule organizing center following nucleation By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Membrane Microtubule
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	centrosome Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule-severing ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 597	597	Spastin		PRO_0000367154
Regions
	Transmembrane	17 – 37	21	Potential
	Domain	91 – 168	78	MIT
	Compositional bias	198 – 252	55	Ser-rich

Sequences

	Sequence		Length	Mass (Da)
	A7T395-1 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: C5477D5F3D9446EF	FASTA	597	66,629
	10 20 30 40 50 60 MPNNDILRPL AIPAKYVGSF LVFLYNGLYF VFVVNLWSRL FGKATKTEVP PLPKIRKLGK 70 80 90 100 110 120 DMASRAPPRR GQSSEDNEDG LPAEIFNVRR HHKQAYAYIA RALEVDEGQG SLETKKRAVE 130 140 150 160 170 180 FYNRGIEEME AGLLIPCIDE GEEWDKARRL QEKMEANLEN TRERMDELVI IFFIIVVALL 190 200 210 220 230 240 VSAGMMDDQP LLSARKTSSE PSQAWDVSKP TGPSYKQSKS YKNSTTVTTK RSQASPSFSS 250 260 270 280 290 300 SSSSVNSTAG SSRTKPAKPA PMAAPRRYNP QVRRTKSTKP AMMAKQSCVD EQKKKISHLK 310 320 330 340 350 360 GIDPKLANII MDEILESGPA VHFSDIAGVD NAKKALQEIV ILPSLRPELW RGDPTLVLFQ 370 380 390 400 410 420 VLPYPPGSSH ITLPRASTAT SFTSCFFSIS KRSSLVHPVV ASFFVKSLED LASILTTSLF 430 440 450 460 470 480 TIDEVDSLLT ERREGEHEHS RRLKTEFLVS FDGVVADPEE RILVMGATNR PQELDDAALR 490 500 510 520 530 540 RMVKRIHIPL PDKETRKVLL TKLLAKHHNP LSGAEIDRLA RMTEHYSGSD LTALARDAAL 550 560 570 580 590 GPIRDLNSDQ LKSMAANEVR NITFQDFVNS LQIIRPSVGP ETLKAYDDWN RLYGSNA

References

[1]

"Sea anemone genome reveals ancestral eumetazoan gene repertoire and genomic organization." Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A., Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J., Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R., Technau U., Martindale M.Q., Rokhsar D.S. Science 317:86-94(2007) [PubMed: 17615350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CH2 X CH6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS470442 Genomic DNA. Translation: EDO29570.1. Different initiation.
RefSeq	XP_001621670.1.
UniGene	Nve.8781
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5500179.
KEGG	nve:NEMVE_v1g144095.
Family and domain databases
InterPro	IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_NEMVE

Accession

Primary (citable) accession number: A7T395

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	October 13, 2009
This is version 15 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)