A7SVT1 (FICD_NEMVE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine monophosphate-protein transferase FICD homolog
EC=2.7.7.n1

Gene names

ORF Names:

v1g194069

Organism

Nematostella vectensis (Starlet sea anemone) [Reference proteome]

Taxonomic identifier

45351 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Anthozoa › Hexacorallia › Actiniaria › Edwardsiidae › Nematostella

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins By similarity.
Catalytic activity	ATP + [protein] = diphosphate + [protein]-AMP.
Enzyme regulation	Adenylyltransferase activity is inhibited by the inhibitory helix present at the N-terminus: Glu-204 binds ATP and competes with ATP-binding at Arg-344, thereby preventing adenylyltransferase activity. Activation dissociates ATP-binding from Glu-204, allowing ordered binding of the entire ATP moiety with the alpha-phosphate in an orientation that is productive for accepting an incoming target hydroxyl side chain By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Domain	The fido domain mediates the adenylyltransferase activity By similarity.
Sequence similarities	Belongs to the fic family. Contains 1 fido domain. Contains 2 TPR repeats.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat TPR repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein adenylyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Adenosine monophosphate-protein transferase FICD homolog		PRO_0000381777
Regions
	Transmembrane	17 – 37	21	Helical; Potential
	Repeat	77 – 110	34	TPR 1
	Repeat	111 – 143	33	TPR 2
	Domain	255 – 390	136	Fido
	Nucleotide binding	230 – 231	2	ATP By similarity
	Nucleotide binding	338 – 340	3	ATP By similarity
	Motif	200 – 205	6	Inhibitory (S/T)XXXE(G/N) motif
Sites
	Binding site	204	1	ATP By similarity
	Binding site	344	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7SVT1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 6C3627BA137B1164	FASTA	427	48,496
	10 20 30 40 50 60 MDSTQIKQGN LRYLIHLLLA SLAGLSIAII VTHAPVFWRL RSSKNTLDPP GFREGLNMLI 70 80 90 100 110 120 PEIHFDAEVQ DPLYGEALAA LKAASAMKHG GKHSKAVKLF QQAVSLAPHH PEILLQYGEF 130 140 150 160 170 180 LEQHDVVQAE HLYNRALTAN PLDSRALANR QRALPKVKQL DQEMLDKIDE KRDKLFSIPA 190 200 210 220 230 240 GSLPMKRAIK EAYFQHIYHS NAIEGNTMTL SMTRAIVETK MAVPGKSILE HNEVLGLDEA 250 260 270 280 290 300 LKYVNSTLIQ KSESITIDDI IEIHRRVLGH AHPLEAGRYR STQVFVSDHV PPAPEDLEKQ 310 320 330 340 350 360 MNAFNDWLLS KDPEILHPIE FAALSHYKLV YIHPFTDGNG RTARLLMNAI LMRAGFPPVI 370 380 390 400 410 420 IRFQDRHDYY EYLNQANHGD IRPFIRFVAR CTERTIDAYL ASTTIYPLGH ERTRELTDAH DEKDPNR

References

[1]

"Sea anemone genome reveals ancestral eumetazoan gene repertoire and genomic organization." Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A., Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J., Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R., Technau U., Martindale M.Q., Rokhsar D.S. Science 317:86-94(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CH2 X CH6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS469844 Genomic DNA. Translation: EDO32183.1.
RefSeq	XP_001624283.1. XM_001624233.1.
UniGene	Nve.18775.
3D structure databases
ProteinModelPortal	A7SVT1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	NEMVEDRAFT_v1g194069-RA; NEMVEDRAFT_v1g194069-PA; NEMVEDRAFT_v1g194069.
GeneID	5503191.
KEGG	nve:NEMVE_v1g194069.
Phylogenomic databases
eggNOG	COG3177.
HOGENOM	HOG000008059.
OMA	MKYINTT.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR003812. Fido. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Pfam	PF02661. Fic. 1 hit. [Graphical view]
SUPFAM	SSF140931. SSF140931. 1 hit.
PROSITE	PS51459. FIDO. 1 hit. PS50005. TPR. 2 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FICD_NEMVE

Accession

Primary (citable) accession number: A7SVT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	October 2, 2007
Last modified:	March 6, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)