Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

v1g214799

Organism
Nematostella vectensis (Starlet sea anemone)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141PhosphateUniRule annotation
Sitei174 – 1741Important for substrate specificityUniRule annotation
Binding sitei192 – 1921Substrate; via amide nitrogenUniRule annotation
Binding sitei193 – 1931PhosphateUniRule annotation
Sitei229 – 2291Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
ORF Names:v1g214799
OrganismiNematostella vectensis (Starlet sea anemone)
Taxonomic identifieri45351 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaAnthozoaHexacoralliaActiniariaEdwardsiidaeNematostella
ProteomesiUP000001593: Unassembled WGS sequence

Subcellular locationi

Cytoplasm UniRule annotation. Nucleus UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298S-methyl-5'-thioadenosine phosphorylasePRO_0000415121Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi45351.JGI214799.

Structurei

3D structure databases

ProteinModelPortaliA7SN31.
SMRiA7SN31. Positions 6-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni56 – 572Phosphate bindingUniRule annotation
Regioni89 – 902Phosphate bindingUniRule annotation
Regioni216 – 2183Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
KOiK00772.
OMAiMTNHTEA.
OrthoDBiEOG771270.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7SN31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANVKVKIGI IGGTGVDNPN IMTDRQEKFV DTPFGKPSEP LITGNIQGVE
60 70 80 90 100
CVLIARHGRK HTVMPTDINY RANVWALKEE GCTHIVVTTA CGSLTEAYRP
110 120 130 140 150
GEIVFPDQII DRTTKRPSTF YDGQTNSPVG VCHIPMHDPY CSVTKQILAN
160 170 180 190 200
EAQKLGIPHH ASGVNVVIEG PRFSTRAESR MFRGLGGEII SMTAMPEVAL
210 220 230 240 250
ANEAGLCYAA IAMVTDYDCW RDDHAPVTVE SVIATFKVNV ANAIKILIAA
260 270 280 290
IPEIAAKDWT EIINERKSQV KPPILDSTWV MFIREYRKIG SDDASKLM
Length:298
Mass (Da):32,721
Last modified:October 2, 2007 - v1
Checksum:i4790357944AF347A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS469716 Genomic DNA. Translation: EDO34894.1.
RefSeqiXP_001626994.1. XM_001626944.1.

Genome annotation databases

EnsemblMetazoaiEDO34894; EDO34894; NEMVEDRAFT_v1g214799.
GeneIDi5506265.
KEGGinve:NEMVE_v1g214799.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS469716 Genomic DNA. Translation: EDO34894.1.
RefSeqiXP_001626994.1. XM_001626944.1.

3D structure databases

ProteinModelPortaliA7SN31.
SMRiA7SN31. Positions 6-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi45351.JGI214799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiEDO34894; EDO34894; NEMVEDRAFT_v1g214799.
GeneIDi5506265.
KEGGinve:NEMVE_v1g214799.

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
KOiK00772.
OMAiMTNHTEA.
OrthoDBiEOG771270.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CH2 X CH6.

Entry informationi

Entry nameiMTAP_NEMVE
AccessioniPrimary (citable) accession number: A7SN31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 2, 2007
Last modified: January 7, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.