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Protein

Ubiquitin-conjugating enzyme E2 S

Gene

v1g237158

Organism
Nematostella vectensis (Starlet sea anemone)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating polyubiquitin chains initiated by the E2 enzyme UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (By similarity).PROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. small conjugating protein transferase activity Source: GO_Central
  4. ubiquitin protein ligase activity Source: GO_Central
  5. ubiquitin protein ligase binding Source: GO_Central

GO - Biological processi

  1. activation of anaphase-promoting complex activity Source: UniProtKB
  2. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  3. cell division Source: UniProtKB-KW
  4. exit from mitosis Source: UniProtKB
  5. free ubiquitin chain polymerization Source: UniProtKB
  6. protein K11-linked ubiquitination Source: UniProtKB
  7. protein polyubiquitination Source: GO_Central
  8. ubiquitin-dependent protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 S (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein S
Ubiquitin-protein ligase S
Gene namesi
ORF Names:v1g237158
OrganismiNematostella vectensis (Starlet sea anemone)
Taxonomic identifieri45351 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaAnthozoaHexacoralliaActiniariaEdwardsiidaeNematostella
ProteomesiUP000001593: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

  1. anaphase-promoting complex Source: UniProtKB
  2. cytoplasm Source: GO_Central
  3. nucleus Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Ubiquitin-conjugating enzyme E2 SPRO_0000390436Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi45351.JGI237158.

Structurei

3D structure databases

ProteinModelPortaliA7SE05.
SMRiA7SE05. Positions 9-159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiA7SE05.
KOiK10583.
OMAiGHKGEIC.
OrthoDBiEOG73JKXD.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7SE05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTSSNVEN FSPQIIKQVA REIHGLTNDP PEGIKVFSND EDITDIQASI
60 70 80 90 100
EGPTGTPYEG GIFKIKLVLG KDFPAAPPKG FFLTKIFHPN VAKNGEICVN
110 120 130 140 150
TLKKDWKPDL GIKQVLLTVK CLLIVPNPES ALNEEAGKLL LERYDDYSKR
160 170 180 190 200
AKMFTEIHAK LSASSSNNIS EGQQESLPGK KRVAVNEKMC DKKKKDKKRA

LKRL
Length:204
Mass (Da):22,620
Last modified:October 2, 2007 - v1
Checksum:i3C1A377D16C59F8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS469633 Genomic DNA. Translation: EDO38105.1.
RefSeqiXP_001630168.1. XM_001630118.1.
UniGeneiNve.13849.

Genome annotation databases

EnsemblMetazoaiEDO38105; EDO38105; NEMVEDRAFT_v1g237158.
GeneIDi5509668.
KEGGinve:NEMVE_v1g237158.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS469633 Genomic DNA. Translation: EDO38105.1.
RefSeqiXP_001630168.1. XM_001630118.1.
UniGeneiNve.13849.

3D structure databases

ProteinModelPortaliA7SE05.
SMRiA7SE05. Positions 9-159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi45351.JGI237158.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiEDO38105; EDO38105; NEMVEDRAFT_v1g237158.
GeneIDi5509668.
KEGGinve:NEMVE_v1g237158.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiA7SE05.
KOiK10583.
OMAiGHKGEIC.
OrthoDBiEOG73JKXD.

Enzyme and pathway databases

UniPathwayiUPA00143.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CH2 X CH6.

Entry informationi

Entry nameiUBE2S_NEMVE
AccessioniPrimary (citable) accession number: A7SE05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: October 2, 2007
Last modified: March 4, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.