A7SE05 (UBE2S_NEMVE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 S
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein S
Ubiquitin-protein ligase S

Gene names

ORF Names:

v1g237158

Organism

Nematostella vectensis (Starlet sea anemone) [Reference proteome]

Taxonomic identifier

45351 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Anthozoa › Hexacorallia › Actiniaria › Edwardsiidae › Nematostella

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating polyubiquitin chains initiated by the E2 enzyme UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of anaphase-promoting complex activity Inferred from sequence or structural similarity. Source: UniProtKB anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW exit from mitosis Inferred from sequence or structural similarity. Source: UniProtKB free ubiquitin chain polymerization Inferred from sequence or structural similarity. Source: UniProtKB protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	anaphase-promoting complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Ubiquitin-conjugating enzyme E2 S		PRO_0000390436
Sites
	Active site	98	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7SE05 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 3C1A377D16C59F8F	FASTA	204	22,620
	10 20 30 40 50 60 MATTSSNVEN FSPQIIKQVA REIHGLTNDP PEGIKVFSND EDITDIQASI EGPTGTPYEG 70 80 90 100 110 120 GIFKIKLVLG KDFPAAPPKG FFLTKIFHPN VAKNGEICVN TLKKDWKPDL GIKQVLLTVK 130 140 150 160 170 180 CLLIVPNPES ALNEEAGKLL LERYDDYSKR AKMFTEIHAK LSASSSNNIS EGQQESLPGK 190 200 KRVAVNEKMC DKKKKDKKRA LKRL

References

[1]

"Sea anemone genome reveals ancestral eumetazoan gene repertoire and genomic organization." Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A., Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J., Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R., Technau U., Martindale M.Q., Rokhsar D.S. Science 317:86-94(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CH2 X CH6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS469633 Genomic DNA. Translation: EDO38105.1.
RefSeq	XP_001630168.1. XM_001630118.1.
UniGene	Nve.13849.
3D structure databases
ProteinModelPortal	A7SE05.
SMR	A7SE05. Positions 9-159.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	NEMVEDRAFT_v1g237158-RA; NEMVEDRAFT_v1g237158-PA; NEMVEDRAFT_v1g237158.
GeneID	5509668.
KEGG	nve:NEMVE_v1g237158.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233455.
KO	K10583.
OMA	PDLGIKH.
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBE2S_NEMVE

Accession

Primary (citable) accession number: A7SE05

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2009
Last sequence update:	October 2, 2007
Last modified:	March 6, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)