Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytosolic Fe-S cluster assembly factor NUBP1 homolog

Gene

v1g236650

Organism
Nematostella vectensis (Starlet sea anemone)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi26 – 261Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi29 – 291Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi35 – 351Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi239 – 2391Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi242 – 2421Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 738ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP1 homologUniRule annotation
Gene namesi
ORF Names:v1g236650
OrganismiNematostella vectensis (Starlet sea anemone)
Taxonomic identifieri45351 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaAnthozoaHexacoralliaActiniariaEdwardsiidaeNematostella
Proteomesi
  • UP000001593 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Cytosolic Fe-S cluster assembly factor NUBP1 homologPRO_0000382613Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains.UniRule annotation

Protein-protein interaction databases

STRINGi45351.NEMVEDRAFT_v1g236650-PA.

Structurei

3D structure databases

ProteinModelPortaliA7RUD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
HOGENOMiHOG000079916.
InParanoidiA7RUD5.
OMAiQRIREFC.
OrthoDBiEOG091G0DY5.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

A7RUD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADVPKDAPE HCPGTESDKS GKVSACQGCP NQQICASSKP APPDPDLGKI
60 70 80 90 100
KERLSSVKHK ILVLSGKGGV GKSTFTAHLA HGLAADEDRQ VAVLDIDICG
110 120 130 140 150
PSIPTVFGLQ GEQVHQSGSG WSPVYVEDNL GVMSVGFLLA KPTDAVIWRG
160 170 180 190 200
PKKNGLIKQF LRDVDWGDAD FLVVDTPPGT SDEHLSIIQY LNQTEVDGAV
210 220 230 240 250
VVTTPQEVSL LDVRKEISFC KKVRLPVIGV VENMSVFVCP NCKKESQIFP
260 270 280 290 300
PTTGGAEKMA VEMKVPFLGR IPLDPRIGRA CDEGKSFLSE IPDSPATSSY
310
KDIIEKIVKY CVASQHQS
Length:318
Mass (Da):34,174
Last modified:October 2, 2007 - v1
Checksum:i61B34B5E65036E00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS469540 Genomic DNA. Translation: EDO44883.1.
RefSeqiXP_001636946.1. XM_001636896.1.
UniGeneiNve.508.

Genome annotation databases

EnsemblMetazoaiEDO44883; EDO44883; NEMVEDRAFT_v1g236650.
GeneIDi5516857.
KEGGinve:NEMVE_v1g236650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS469540 Genomic DNA. Translation: EDO44883.1.
RefSeqiXP_001636946.1. XM_001636896.1.
UniGeneiNve.508.

3D structure databases

ProteinModelPortaliA7RUD5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi45351.NEMVEDRAFT_v1g236650-PA.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiEDO44883; EDO44883; NEMVEDRAFT_v1g236650.
GeneIDi5516857.
KEGGinve:NEMVE_v1g236650.

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
HOGENOMiHOG000079916.
InParanoidiA7RUD5.
OMAiQRIREFC.
OrthoDBiEOG091G0DY5.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNUBP1_NEMVE
AccessioniPrimary (citable) accession number: A7RUD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: October 2, 2007
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.