Probable polygalacturonase - Vitis vinifera (Grape)

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A7PZL3 (PGLR_VITVI) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 20. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable polygalacturonase
Short name=PG
EC=3.2.1.15

Alternative name(s):
Pectinase

Gene names

ORF Names:

GSVIVT00026920001, LOC100243180

Organism

Vitis vinifera (Grape)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › Vitales › Vitaceae › Vitis

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 28 family.

Contains 4 PbH1 repeats.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

491

Probable polygalacturonase

PRO_0000363726

Regions

Transmembrane

21

Helical; Potential

Repeat

27

PbH1 1

Repeat

22

PbH1 2

Repeat

22

PbH1 3

Repeat

22

PbH1 4

Sites

Active site

1

Proton donor By similarity UniProtKB Q7M1E7

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

A7PZL3 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: BFEB4EC7843D2BB6
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491

53,489

        10         20         30         40         50         60 
MVETHKKKIA NLTQPIVSFY CFQVVSVLVA VVLLLSVSRG ECRKGRILEA LEYSAISCRA 

        70         80         90        100        110        120 
HSASLVDFGG VGDGQTLNTK AFQDAVSELS KYGSEGGAQL YVPAGKWLTG SFSLTSHFTL 

       130        140        150        160        170        180 
FLHRDAVLLA SQDISQWPVI KPLPSYGRGR DAAAGRYTSL IFGTNLTDVI ITGDNGTIDG 

       190        200        210        220        230        240 
QGGLWWQRFH GGKLKYTRPY LIELMYSADI QISNLTLLNS PSWNVHPVYS RNILIQGITI 

       250        260        270        280        290        300 
LAPVRSPNTD GINPDSCTNT RIEDCYIVSG DDCVAVKSGW DEYGIAYGMP TKQLVIRRLT 

       310        320        330        340        350        360 
CISPYSAVIA LGSEMSGGIQ DVRAEDIVAI NSESGIRIKT GIGRGGYVKD IYVRGMTMKT 

       370        380        390        400        410        420 
MKWAFWMTGN YGSHADNHYD PKAFPVIQGI NYRDMVAENV SMAARLEGIP SDPFTGICIS 

       430        440        450        460        470        480 
NVTIHLAAKA KKVPWTCTDV EGISSGVTPT PCSTLPDQGP EKTSLCNFPA ESLPIDTVEL 

       490 
QKCSYGINYY P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla." Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A., Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F., Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J. Wincker P. Nature 449:463-467(2007) [PubMed: 17721507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Pinot noir / PN40024.
[2]	Almagro L., Belchi-Navarro S., Pedreno M.A. Submitted (JUL-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 324-337.

Cross-references

3D structure databases
ProteinModelPortal	A7PZL3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 4 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_VITVI

Accession

Primary (citable) accession number: A7PZL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	October 2, 2007
Last modified:	September 21, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents