A7PW81 (PGIP_VITVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polygalacturonase inhibitor

Alternative name(s):
Polygalacturonase-inhibiting protein
Short name=PGIG

Gene names

Name:	pgip
ORF Names:	GSVIVT00025506001, LOC100232865

Organism

Vitis vinifera (Grape)

Taxonomic identifier

29760 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › rosids incertae sedis › Vitales › Vitaceae › Vitis

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of fungal polygalacturonase. It is an important factor for plant resistance to phytopathogenic fungi.
Subcellular location	Secreted › cell wall By similarity. Membrane; Peripheral membrane protein By similarity UniProtKB P58822.
Sequence similarities	Belongs to the polygalacturonase-inhibiting protein family. Contains 7 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cell wall Membrane Secreted
Domain	Leucine-rich repeat Repeat Signal
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type cell wall Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	polygalacturonase inhibitor activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 333	306	Polygalacturonase inhibitor		PRO_0000363745
Regions
	Repeat	121 – 144	24	LRR 1
	Repeat	147 – 169	23	LRR 2
	Repeat	171 – 192	22	LRR 3
	Repeat	196 – 217	22	LRR 4
	Repeat	219 – 241	23	LRR 5
	Repeat	266 – 288	23	LRR 6
	Repeat	289 – 311	23	LRR 7
Amino acid modifications
	Glycosylation	109	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	147	1	N-linked (GlcNAc...) Potential
	Glycosylation	157	1	N-linked (GlcNAc...) Potential
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Glycosylation	294	1	N-linked (GlcNAc...) Potential
	Disulfide bond	30 ↔ 60		By similarity UniProtKB P58822
	Disulfide bond	61 ↔ 68		By similarity UniProtKB P58822
	Disulfide bond	301 ↔ 323		By similarity UniProtKB P58822
	Disulfide bond	325 ↔ 332		By similarity UniProtKB P58822
Experimental info
	Sequence conflict	47	1	N → T in AAK14075. Ref.1
	Sequence conflict	135	1	S → F in AAK14075. Ref.1
	Sequence conflict	177	1	L → I in AAK14075. Ref.1
	Sequence conflict	199 – 201	3	GLH → YLY in AAM74142. Ref.2
	Sequence conflict	221	1	N → T in AAM74142. Ref.2
	Sequence conflict	234	1	L → P in AAM74142. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	A7PW81 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 0D4C502A6B93EF2D	FASTA	333	36,929
	10 20 30 40 50 60 METSKLFLLS SSLLLVLLAT RPCPSLSERC NPKDKKVLLQ IKKALDNPYI LASWNPNTDC 70 80 90 100 110 120 CGWYCVECDL TTHRINSLTI FSGQLSGQIP DAVGDLPFLE TLIFRKLSNL TGQIPPAIAK 130 140 150 160 170 180 LKHLKMVRLS WTNLSGPVPA FFSELKNLTY LDLSFNNLSG PIPGSLSLLP NLGALHLDRN 190 200 210 220 230 240 HLTGPIPDSF GKFAGSTPGL HLSHNQLSGK IPYSFRGFDP NVMDLSRNKL EGDLSIFFNA 250 260 270 280 290 300 NKSTQIVDFS RNLFQFDLSR VEFPKSLTSL DLSHNKIAGS LPEMMTSLDL QFLNVSYNRL 310 320 330 CGKIPVGGKL QSFDYDSYFH NRCLCGAPLQ SCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a grapevine gene coding for polygalacturonase inhibiting protein." Bezier A., Lambert B., Baillieul F. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Chardonnay. Tissue: Leaf.
[2]	"The isolation and characterization of a gene encoding a polygalacturonase-inhibiting protein (PGIP) from Vitis vinifera L." De Ascensao A.R., Pretorius I.S., Bellstedt D.U., Burger J.T., Vivier M.A. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Pinotage.
[3]	"The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla." Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A., Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F., Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J. , Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E., Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M., Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M., Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E., Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M., Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G., Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F., Wincker P. Nature 449:463-467(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Pinot noir / PN40024.
[4]	Almagro L., Belchi-Navarro S., Pedreno M.A. Submitted (JUL-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 172-179.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF305093 mRNA. Translation: AAK14075.1. AF499451 Genomic DNA. Translation: AAM74142.1.
RefSeq	XP_002282695.1. XM_002282659.1.
UniGene	Vvi.9.
3D structure databases
ProteinModelPortal	A7PW81.
ModBase	Search...
Protein-protein interaction databases
STRING	29760.GSVIVG00025506001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100232865.
KEGG	vvi:100232865.
Phylogenomic databases
eggNOG	NOG288515.
Gene expression databases
ArrayExpress	A7PW81.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR013210. LRR-contain_N2. [Graphical view]
Pfam	PF00560. LRR_1. 1 hit. PF08263. LRRNT_2. 1 hit. [Graphical view]
PROSITE	PS51450. LRR. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGIP_VITVI

Accession

Primary (citable) accession number: A7PW81
Secondary accession number(s): Q8LKV2, Q9AXP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	October 2, 2007
Last modified:	April 3, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program