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Protein

Nitrogenase iron protein

Gene

nifH

Organism
Roseiflexus castenholzii (strain DSM 13941 / HLO8)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.UniRule annotation

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi95 – 951Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi130 – 1301Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 158ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRCAS383372:GH89-4098-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron proteinUniRule annotation (EC:1.18.6.1UniRule annotation)
Alternative name(s):
Nitrogenase Fe proteinUniRule annotation
Nitrogenase component IIUniRule annotation
Nitrogenase reductaseUniRule annotation
Gene namesi
Name:nifHUniRule annotation
Ordered Locus Names:Rcas_4043
OrganismiRoseiflexus castenholzii (strain DSM 13941 / HLO8)
Taxonomic identifieri383372 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesRoseiflexineaeRoseiflexaceaeRoseiflexus
Proteomesi
  • UP000000263 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Nitrogenase iron proteinPRO_1000211887Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei98 – 981ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseUniRule annotation

Post-translational modificationi

The reversible ADP-ribosylation of Arg-98 inactivates the nitrogenase reductase and regulates nitrogenase activity.UniRule annotation

Keywords - PTMi

ADP-ribosylation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi383372.Rcas_4043.

Structurei

3D structure databases

ProteinModelPortaliA7NR80.
SMRiA7NR80. Positions 1-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228826.
KOiK02588.
OMAiNKMFVIP.
OrthoDBiPOG091H0230.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7NR80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQVAFYGKG GIGKSTTQQN TAAALASMGY RLMVVGCDPK ADCTRLLLRG
60 70 80 90 100
VRQPSVLDTL RDVGPESVQL EKVVVQGYGG VKCVESGGPE PGVGCGGRGV
110 120 130 140 150
ITAIQTLETL GAYKDDLDYV FYDVLGDVVC GGFAMPIREG YAEEIYIVCS
160 170 180 190 200
GEYMALFAAN NICKGIKKFA ERGYARLGGL VCNSRLVENE QALVKEFARR
210 220 230 240 250
LNTKMIHFIP RSKDVQRAEI NKKTVIDYDP DLPQAQEYRE LARKIDENDE
260 270
FTIPTPITQE ELEDLMREYG IVD
Length:273
Mass (Da):30,112
Last modified:October 2, 2007 - v1
Checksum:iFA1E14839440E539
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000804 Genomic DNA. Translation: ABU60076.1.
RefSeqiWP_012122497.1. NC_009767.1.

Genome annotation databases

EnsemblBacteriaiABU60076; ABU60076; Rcas_4043.
KEGGirca:Rcas_4043.
PATRICi23346954. VBIRosCas91182_4512.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000804 Genomic DNA. Translation: ABU60076.1.
RefSeqiWP_012122497.1. NC_009767.1.

3D structure databases

ProteinModelPortaliA7NR80.
SMRiA7NR80. Positions 1-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi383372.Rcas_4043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU60076; ABU60076; Rcas_4043.
KEGGirca:Rcas_4043.
PATRICi23346954. VBIRosCas91182_4512.

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228826.
KOiK02588.
OMAiNKMFVIP.
OrthoDBiPOG091H0230.

Enzyme and pathway databases

BioCyciRCAS383372:GH89-4098-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIFH_ROSCS
AccessioniPrimary (citable) accession number: A7NR80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: October 2, 2007
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.