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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Roseiflexus castenholzii (strain DSM 13941 / HLO8)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi493 – 4931MagnesiumUniRule annotation
Metal bindingi499 – 4991MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciRCAS383372:GH89-3633-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Rcas_3588
OrganismiRoseiflexus castenholzii (strain DSM 13941 / HLO8)
Taxonomic identifieri383372 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesRoseiflexineaeRoseiflexaceaeRoseiflexus
ProteomesiUP000000263: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 746746Polyribonucleotide nucleotidyltransferasePRO_0000381916Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi383372.Rcas_3588.

Structurei

3D structure databases

ProteinModelPortaliA7NPZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini560 – 61960KHUniRule annotationAdd
BLAST
Domaini629 – 69870S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7NPZ1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSERRIHSVS MDLAGRTLTL ETGRFAEQAN GAVVVRYGDT MLLATAVASK
60 70 80 90 100
EPRTDTDFFP LTVDYEEKMY AAGKIPGSFF KREGKPTEGA ILTARLTDRP
110 120 130 140 150
LRPLFPEGYR NEVQIIVTTF SIDMVNDPAP LSIIAASAAL AISDIPFLGP
160 170 180 190 200
VGAVQVGYID GALQINPPMP NMANSDLDLV VAGTKEAVLM VEAGANELPE
210 220 230 240 250
EVMLEAVIQG HQVCKQICDL QNELVKLAGR PKKEFVPPPV DTSLEEAIQQ
260 270 280 290 300
WLGNRLYEAI TDANKMVRDA QTEALKREVI AHFTADEPEE ELEARTAAVS
310 320 330 340 350
AAFENILYEE VRRMILERGE RVDGRGPKDI RPISVEVGLI PRVHGSGLFT
360 370 380 390 400
RGQTQVLTLA TLGSPAEEQR LDDLGIETAK RYIHHYNFPP FSTGEIRRLG
410 420 430 440 450
SPRRRDIGHG ALAERSLLAV LPPKEEFPYT MRLVSETLSS NGSSSMASVC
460 470 480 490 500
GSSLALMDAG VPIRAPVAGI AMGLITGKDG RWRVLTDIQG IEDHLGDMDF
510 520 530 540 550
KVAGTAKGIT GLQMDIKTTG ITYEIMREAF AQAREGRLHV LEKMNAVISE
560 570 580 590 600
PRKELSPYAP RIITLQINPE KIGALIGPGG KTIRSITEAT GAQIDIEEDG
610 620 630 640 650
RVYISTADAA AAQQAVAMVE ALTREIKVGD IFLGKVVRIM PFGAFVNLAP
660 670 680 690 700
GKDGMVHVSE LDVGRVENVE DVIKMGDEIN VMVIGIEPGT GKVSLSRRAL
710 720 730 740
LTGETAEDRR AAGAGRGLRD GGRSSGSERS GDRSPRSDDR PRPRRR
Length:746
Mass (Da):80,953
Last modified:October 2, 2007 - v1
Checksum:iD713881A9D965210
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000804 Genomic DNA. Translation: ABU59637.1.
RefSeqiYP_001433655.1. NC_009767.1.

Genome annotation databases

EnsemblBacteriaiABU59637; ABU59637; Rcas_3588.
GeneIDi5541089.
KEGGirca:Rcas_3588.
PATRICi23345926. VBIRosCas91182_4008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000804 Genomic DNA. Translation: ABU59637.1.
RefSeqiYP_001433655.1. NC_009767.1.

3D structure databases

ProteinModelPortaliA7NPZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi383372.Rcas_3588.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU59637; ABU59637; Rcas_3588.
GeneIDi5541089.
KEGGirca:Rcas_3588.
PATRICi23345926. VBIRosCas91182_4008.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciRCAS383372:GH89-3633-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13941 / HLO8.

Entry informationi

Entry nameiPNP_ROSCS
AccessioniPrimary (citable) accession number: A7NPZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: October 2, 2007
Last modified: January 7, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.