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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Roseiflexus castenholzii (strain DSM 13941 / HLO8)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. 6-phosphofructokinase (Rcas_1875), ATP-dependent 6-phosphofructokinase (pfkA), 6-phosphofructokinase (Rcas_2513)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei18 – 181ATP; via amide nitrogenUniRule annotation
Metal bindingi128 – 1281Magnesium; catalyticUniRule annotation
Sitei129 – 1291Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei152 – 1521Proton acceptorUniRule annotation
Binding sitei187 – 1871Substrate; shared with dimeric partnerUniRule annotation
Binding sitei247 – 2471SubstrateUniRule annotation
Binding sitei290 – 2901Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 872ATPUniRule annotation
Nucleotide bindingi127 – 1304ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciRCAS383372:GH89-2541-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Rcas_2515Imported
OrganismiRoseiflexus castenholzii (strain DSM 13941 / HLO8)Imported
Taxonomic identifieri383372 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesRoseiflexineaeRoseiflexaceaeRoseiflexus
Proteomesi
  • UP000000263 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi383372.Rcas_2515.

Structurei

3D structure databases

ProteinModelPortaliA7NM40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 322313PFKInterPro annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1523Substrate bindingUniRule annotation
Regioni194 – 1963Substrate bindingUniRule annotation
Regioni296 – 2994Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
KOiK00850.
OMAiRGLYNND.
OrthoDBiPOG091H01AC.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7NM40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIDTASRLR IAICTGGGDA PGLNAVIRAV VLSALNRGWE CYGIRDGFNG
60 70 80 90 100
ILSPKSYPRG GVIPLTRDTV HGITPLGGTI IGTTNRGNPL RYPTLLPDGT
110 120 130 140 150
VIERDRTDEI VNGLAVYGID ALISVGGDGS MEIASALTRK GVRVIGVPKT
160 170 180 190 200
IDNDLDGTVA TFGFDTAVAF ATEAIDRLHT TAEAHQRVMV VEVMGRYAGW
210 220 230 240 250
IALNAGVSGT AHAVLIPEIP YDIHKVAAKI KSREAEGRPY SVVVVAEGAR
260 270 280 290 300
PIEGSVSLIA PKEAGRAERL GGIGERVARE LEALTGRETR VVVLGHLLRG
310 320 330 340 350
GTPTTFDRLL ALRFGAAAVR ALEEGQSGVM VALDPPTVRY VPLEEATRRM
360 370
KTVPLDSDTM LTARDLGISF GD
Length:372
Mass (Da):39,334
Last modified:October 2, 2007 - v1
Checksum:iE4BAAEE819BFFB3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000804 Genomic DNA. Translation: ABU58595.1.
RefSeqiWP_012121019.1. NC_009767.1.

Genome annotation databases

EnsemblBacteriaiABU58595; ABU58595; Rcas_2515.
KEGGirca:Rcas_2515.
PATRICi23343523. VBIRosCas91182_2826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000804 Genomic DNA. Translation: ABU58595.1.
RefSeqiWP_012121019.1. NC_009767.1.

3D structure databases

ProteinModelPortaliA7NM40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi383372.Rcas_2515.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU58595; ABU58595; Rcas_2515.
KEGGirca:Rcas_2515.
PATRICi23343523. VBIRosCas91182_2826.

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
KOiK00850.
OMAiRGLYNND.
OrthoDBiPOG091H01AC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciRCAS383372:GH89-2541-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiA7NM40_ROSCS
AccessioniPrimary (citable) accession number: A7NM40
Entry historyi
Integrated into UniProtKB/TrEMBL: October 2, 2007
Last sequence update: October 2, 2007
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.