A7NI01 (PIMT_ROSCS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-L-isoaspartate O-methyltransferase
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Short name=PIMT

Gene names

Name:	pcm
Ordered Locus Names:	Rcas_0989

Organism

Roseiflexus castenholzii (strain DSM 13941 / HLO8) [Complete proteome] [HAMAP]

Taxonomic identifier

383372 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Chloroflexales › Chloroflexaceae › Roseiflexus › Roseiflexus castenholzii

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity. HAMAP-Rule MF_00090
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP-Rule MF_00090
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00090.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Protein-L-isoaspartate O-methyltransferase HAMAP-Rule MF_00090		PRO_0000351929
Sites
	Active site	60	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7NI01 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: DA8F91B84B22A755	FASTA	218	23,809
	10 20 30 40 50 60 MDFTNERRAM IDLLIRRGIS DQRVLDAMAQ TPRHVFVPAH ERSHAYSDQA LPIGEGQTIS 70 80 90 100 110 120 QPYMVALMIE ALQLAPTDRV LEIGAGSGYA AAVLSRIVAK VHTIECREAL AASAAALLRT 130 140 150 160 170 180 LGYDNVTVHV GDGTQGLPDY APFDAILVSA ASPWVPAPLR EQLASSGRLV IPVGGRQAQI 190 200 210 LLRLRRTGDT LRTERLCDVR FVPLIGGHAW TTEHYPER

References

[1]

"Complete sequence of Roseiflexus castenholzii DSM 13941." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Bryant D.A., Hanada S., Tsukatani Y., Richardson P. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13941 / HLO8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000804 Genomic DNA. Translation: ABU57098.1.
RefSeq	YP_001431116.1. NC_009767.1.
3D structure databases
ProteinModelPortal	A7NI01.
ModBase	Search...
Protein-protein interaction databases
STRING	383372.Rcas_0989.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABU57098; ABU57098; Rcas_0989.
GeneID	5538455.
KEGG	rca:Rcas_0989.
PATRIC	23340107. VBIRosCas91182_1133.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2518.
HOGENOM	HOG000257189.
KO	K00573.
OMA	DGYQGWP.
ProtClustDB	CLSK974990.
Enzyme and pathway databases
BioCyc	RCAS383372:GH89-999-MONOMER.
Family and domain databases
HAMAP	MF_00090. PIMT.
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PTHR11579. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_ROSCS

Accession

Primary (citable) accession number: A7NI01

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 2, 2007
Last modified:	May 29, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program