A7NG78 (PANC_ROSCS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate synthetase
Short name=PS
EC=6.3.2.1

Alternative name(s):
Pantoate--beta-alanine ligase
Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	Rcas_0333

Organism

Roseiflexus castenholzii (strain DSM 13941 / HLO8) [Complete proteome] [HAMAP]

Taxonomic identifier

383372 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Roseiflexus

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm Potential HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Pantothenate synthetase HAMAP MF_00158		PRO_1000076862
Regions
	Nucleotide binding	27 – 34	8	ATP By similarity
	Nucleotide binding	144 – 147	4	ATP By similarity
	Nucleotide binding	181 – 184	4	ATP By similarity
Sites
	Active site	34	1	Proton donor By similarity
	Binding site	58	1	Beta-alanine By similarity
	Binding site	58	1	Pantoate By similarity
	Binding site	150	1	Pantoate By similarity
	Binding site	173	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7NG78 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 5B1BCD6AF24ED4AB	FASTA	278	30,590
	10 20 30 40 50 60 MRVIATIGEF RAARAAMHGA VGLVPTMGYL HEGHLSLVRR ARAENDHVIV TIFVNPTQFG 70 80 90 100 110 120 PSEDLSRYPR DLPRDLALLE AEKIDVVFAP NVAEMYPPGF GTFVDVGPIA APLEGAARPG 130 140 150 160 170 180 HFRGVATVVC KLFNITTPHR AYFGQKDAQQ TLVIRRMTLD LNLPVEIIVC PIVREPDGLA 190 200 210 220 230 240 MSSRNVYLNP KERRAATVLF RALQAVQERF RAGERNGDAL RAAMRAVIDA EPLAHPDYVS 250 260 270 IADLDDLHEL DRVTSRALAS LAVRIGTTRL IDNCILEA

References

[1]

"Complete sequence of Roseiflexus castenholzii DSM 13941." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Bryant D.A., Hanada S., Tsukatani Y., Richardson P. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13941 / HLO8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000804 Genomic DNA. Translation: ABU56465.1.
RefSeq	YP_001430483.1. NC_009767.1.
3D structure databases
ProteinModelPortal	A7NG78.
SMR	A7NG78. Positions 1-276.
ModBase	Search...
Protein-protein interaction databases
STRING	A7NG78.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5537795.
GenomeReviews	Gene locus Rcas_0333 in contig CP000804_GR.
KEGG	rca:Rcas_0333.
PATRIC	23338605. VBIRosCas91182_0385.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0414.
HOGENOM	HBG428839.
OMA	LNMPIQI.
ProtClustDB	PRK00380.
Enzyme and pathway databases
BioCyc	RCAS383372:RCAS_0333-MONOMER.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR004821. Cyt_trans-rel. IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01918.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. Cyt_tran_rel. 1 hit. TIGR00018. PanC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_ROSCS

Accession

Primary (citable) accession number: A7NG78

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program