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A7NG68 (A7NG68_ROSCS) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP MF_02003
Gene names
Name:ileS HAMAP MF_02003
Ordered Locus Names:Rcas_0322
OrganismRoseiflexus castenholzii (strain DSM 13941 / HLO8) [Complete proteome] [HAMAP] EMBL ABU56455.1
Taxonomic identifier383372 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Roseiflexus

Protein attributes

Sequence length1079 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
A7NG68 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 34C54228802842B5

FASTA1,079121,960
        10         20         30         40         50         60 
MAFQPVDTRV NFPRLEEQIL DLWQRKQIVE RSLESGDKPF VFYEGPPTAN GRPGIHHAIS 

        70         80         90        100        110        120 
RIFKDVICRY RAMQGYKIIG RREGWDTHGL PVEIEVEKKL GFSGKPDIEK YGVAEFNAQC 

       130        140        150        160        170        180 
RQSVWEYIQD WRQFTRRIAY WVSEDAYITY ENDYIESLWW IFRQLWDRGL LFRDYKVSMH 

       190        200        210        220        230        240 
CPRCGTSLSD HEVSLGFKDD VDDPSVWVKF RVRETSHEAL RGASFVAWTT TPWTLPANVA 

       250        260        270        280        290        300 
LAVKPDAAYV VVSHDGERFV LAEALLHSVL GDGVTVERTF QGADLVGTRY ENLFDGVPAP 

       310        320        330        340        350        360 
GDTVDWSRAY TVVADDFVSL EDGTGIVHIA PAYGDLEIGR KYGLPTLFSV DLAGNVLPEF 

       370        380        390        400        410        420 
ASLGFAGKFF KQADPDITRN LKARGLLLRS GRVKHSYPFC WRCDTPLLYY AKRSWYIRTT 

       430        440        450        460        470        480 
AMKDRLVANN LLIHWVPEHI RDGRFGNWLE NNIDWAVSRE RYWGTPIPIW MAEDGYAECI 

       490        500        510        520        530        540 
GSLAELEQKV GRTLKDLELH RPYVDEITWV DPQHGLMRRI PDVADCWFDS GAMPIAQWHY 

       550        560        570        580        590        600 
PMENTEIYEL AKQADYICEA VDQTRGWFYT LHAISTLLFD RPAFKNVICL GHILDAEGQK 

       610        620        630        640        650        660 
MSKSRGNVVD PWTVMNEHGA DALRWYLFTA APPGSPRRFS AGLVAESLRK FLLTLWNTYA 

       670        680        690        700        710        720 
FFATYANLDG WTPTKDGVDQ HLDPATLVPI DRWALARLNS LVRTVTDAFE AYDVTTAARA 

       730        740        750        760        770        780 
IEDFVDELSN WYVRRNRRRF WKSEHDRDKQ AAYATLYTCL LTVAKLAAPF VPFVAEAIYR 

       790        800        810        820        830        840 
NLVGLEGHEG QTDTPESVHL VAWPQVNEAL LEERLVADTE VLLKAVSVGR AARRAAGIKV 

       850        860        870        880        890        900 
RQPLRELWVR ALTPTALEGL RRFEAELRDE LNVKAVRYLD STATLVEYRL KPNLRLVGRK 

       910        920        930        940        950        960 
YGKLVPAITA ALRDLTGDAA RAAAQAVEAG ETITLTLEGQ AIDLLPEEVL VESSSPEGYA 

       970        980        990       1000       1010       1020 
VAEDGGVLVA LDTTLTPELT MEGLARELVR NIQDARKDAG FAISDRIIVF LGGADGDAAV 

      1030       1040       1050       1060       1070 
EAMLRAWGDY VREETLADDL RLCAPPDHVH TTSLELDEGR TLTIGVSRGG TETRPHGYV

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References

[1]"Complete sequence of Roseiflexus castenholzii DSM 13941."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Bryant D.A., Hanada S., Tsukatani Y., Richardson P.
Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13941 / HLO8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000804 Genomic DNA. Translation: ABU56455.1.
RefSeqYP_001430473.1. NC_009767.1.

3D structure databases

ProteinModelPortalA7NG68.
SMRA7NG68. Positions 8-838.
ModBaseSearch...

Protein-protein interaction databases

STRINGA7NG68.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5537784.
GenomeReviewsGene locus Rcas_0322 in contig CP000804_GR.
KEGGrca:Rcas_0322.
PATRIC23338583. VBIRosCas91182_0374.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHBG577712.
OMAPYITYDN.
ProtClustDBPRK06039.

Enzyme and pathway databases

BioCycRCAS383372:RCAS_0322-MONOMER.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameA7NG68_ROSCS
AccessionPrimary (citable) accession number: A7NG68
Entry history
Integrated into UniProtKB/TrEMBL: October 2, 2007
Last sequence update: October 2, 2007
Last modified: January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info