Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A7NG29 (MDH_ROSCS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:Rcas_0283
OrganismRoseiflexus castenholzii (strain DSM 13941 / HLO8) [Complete proteome] [HAMAP]
Taxonomic identifier383372 [NCBI]
Taxonomic lineageBacteriaChloroflexiChloroflexalesChloroflexaceaeRoseiflexus

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Malate dehydrogenase HAMAP-Rule MF_00487
PRO_1000191656

Regions

Nucleotide binding9 – 146NAD By similarity
Nucleotide binding118 – 1203NAD By similarity

Sites

Active site1751Proton acceptor By similarity
Binding site331NAD By similarity
Binding site821Substrate By similarity
Binding site881Substrate By similarity
Binding site951NAD By similarity
Binding site1201Substrate By similarity
Binding site1511Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A7NG29 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: B6967C27757E2A53

FASTA30932,941
        10         20         30         40         50         60 
MRPKISIIGA GFVGSTAAHW IASKELGDVV LVDIIDGVPQ GKGLDLLQAG PIEGFDVKIT 

        70         80         90        100        110        120 
GTNDYAATAN SDIIIVTSGA PRKPGMSRED LIRVNADITR DCISKAAPLS PDAVIIMVNN 

       130        140        150        160        170        180 
PLDTMTYLAK QVSGFPKNRV VGQAGVLDTA RYRTFIAMEA GVSVEDIQAM LMGGHGDEMV 

       190        200        210        220        230        240 
PLPRFTTISG IPVTEFISKE RLDAIIERTR KGGGEIVNLL KTGSAYYAPS AATVQMVEAI 

       250        260        270        280        290        300 
LRDKKRVLPC ACYLEGEYGL NDIYFGVPCV LGAGGVERVL ELPLNDEEMA LVRKSAEAVS 


SSIATLKQM 

« Hide

References

[1]"Complete sequence of Roseiflexus castenholzii DSM 13941."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Bryant D.A., Hanada S., Tsukatani Y., Richardson P.
Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13941 / HLO8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000804 Genomic DNA. Translation: ABU56416.1.
RefSeqYP_001430434.1. NC_009767.1.

3D structure databases

ProteinModelPortalA7NG29.
SMRA7NG29. Positions 2-309.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING383372.Rcas_0283.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU56416; ABU56416; Rcas_0283.
GeneID5537745.
KEGGrca:Rcas_0283.
PATRIC23338499. VBIRosCas91182_0332.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213794.
KOK00024.
OMAGANSYEA.
OrthoDBEOG6091FG.
ProtClustDBPRK06223.

Enzyme and pathway databases

BioCycRCAS383372:GH89-289-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01763. MalateDH_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_ROSCS
AccessionPrimary (citable) accession number: A7NG29
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 2, 2007
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families