A7NFB8 (DNAA_ROSCS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	Rcas_0001

Organism

Roseiflexus castenholzii (strain DSM 13941 / HLO8) [Complete proteome] [HAMAP]

Taxonomic identifier

383372 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Roseiflexus

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_1000079959
Regions
	Nucleotide binding	181 – 188	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A7NFB8 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: FF31B7DE6AF4D453	FASTA	480	54,091
	10 20 30 40 50 60 MNLTKVWNTT LGSLQVQLPR HEYNTWVRGA NLLDIDNGVA IIRAPNAFIK EGLESRYLTT 70 80 90 100 110 120 LREQLGSVVG FPVDVRIVLA TSESERIDGA SINGRHAARD TRKSDHHAPL SGGYGNGISH 130 140 150 160 170 180 PLERASVHQL ELHRAVRSSM LNPRYTFDRF IIGPSNRLAN AACMAVAEHP AQAYNPLFLY 190 200 210 220 230 240 GGVGLGKTHL LHAIGNFVLD RDPEVNVLYV SSETFTNDLI NSIRRQQTEE FRIRYRNIDI 250 260 270 280 290 300 LLIDDIQFIA GKEQTQEEFF HTFNTLHSAG KQIIISSDRS PKAILTLEER LRSRFEWGLI 310 320 330 340 350 360 VDVQMPDLET RTAILRAKAE QSPVPVPQPV IDFLAQRIQS HIRELEGCLN RVTAYAQMYN 370 380 390 400 410 420 IPVTIEVATA ALSELLDTNR RKRVTPEAIL REVAAFYSVD LRSLQGRGRS RNIVTPRHVA 430 440 450 460 470 480 MYLLREETDS SLMEIGQLLG GRDHTTVMYG CDKIAEEINT DARLRQEVAA IRERLMNSAV

References

[1]

"Complete sequence of Roseiflexus castenholzii DSM 13941." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Bryant D.A., Hanada S., Tsukatani Y., Richardson P. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13941 / HLO8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000804 Genomic DNA. Translation: ABU56140.1.
RefSeq	YP_001430158.1. NC_009767.1.
3D structure databases
ProteinModelPortal	A7NFB8.
ModBase	Search...
Protein-protein interaction databases
STRING	A7NFB8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5541967.
GenomeReviews	Gene locus Rcas_0001 in contig CP000804_GR.
KEGG	rca:Rcas_0001.
PATRIC	23337826. VBIRosCas91182_0001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0593.
HOGENOM	HBG695666.
OMA	CHTLNAL.
ProtClustDB	PRK00149.
Enzyme and pathway databases
BioCyc	RCAS383372:RCAS_0001-MONOMER.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_ROSCS

Accession

Primary (citable) accession number: A7NFB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program