A7NE12 (GLPK_FRATF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	FTA_1739

Organism

Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) [Complete proteome] [HAMAP]

Taxonomic identifier

458234 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Glycerol kinase HAMAP MF_00186		PRO_1000058448
Regions
	Nucleotide binding	412 – 416	5	ATP By similarity
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	17	1	ATP By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	268	1	ATP By similarity
	Binding site	311	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7NE12 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 853AC3C05CEBDB80	FASTA	502	55,818
	10 20 30 40 50 60 MSKDFILAID QGTTSSRAII FDKKGNIRKI AQKEFTQIYP KSGWVEHDAM EIWGTQSGVM 70 80 90 100 110 120 REALEFGRVK PDQIAAIGIT NQRETVVVWD KETGDPVYNA IVWQCRRTSS ICDEIKRDPQ 130 140 150 160 170 180 FVKYIKENTG LVVDAYFSGT KVKWILDNVE GAREKANAGK LLMGTIDTWL IWNLTRGKVH 190 200 210 220 230 240 ATDYSNASRT MLFNINSLEW DKKILDYLNI PESMLPEVKN SSEVFGVTDS HTLGGAEIPI 250 260 270 280 290 300 AGVAGDQHAA LFGHCCFEKG MAKNTYGTGC FALMNVGDKP VYSDEGLLTT IAWAENGKPT 310 320 330 340 350 360 YALEGSIFIV GAVIQWIRDG LGLVRSAEDS EYYATKIDST NGVYLVPAFV GLGTPYWDMY 370 380 390 400 410 420 ARGTIVGITR DTKREHIIRA ALEAIAYQAK DVLECMKEDT GLDLAGLRVD GGAVQNNFLM 430 440 450 460 470 480 QFQSDILQSE ISKPKINEIT SLGAVFLAGL AVGFWKDKQE LKSILTTEKV FEPQKDSQAV 490 500 AHDYRGWKKA VERSKAWAEC YS

References

[1]

"Complete genome sequence of Francisella tularensis subspecies holarctica FTNF002-00." Barabote R.D., Xie G., Brettin T.S., Hinrichs S.H., Fey P.D., Jay J.J., Engle J.L., Godbole S.D., Noronha J.M., Scheuermann R.H., Zhou L.W., Lion C., Dempsey M.P. PLoS ONE 4:E7041-E7041(2009) [PubMed: 19756146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FTNF002-00 / FTA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000803 Genomic DNA. Translation: ABU62214.1.
RefSeq	YP_001429171.1. NC_009749.1.
3D structure databases
ProteinModelPortal	A7NE12.
SMR	A7NE12. Positions 3-499.
ModBase	Search...
Protein-protein interaction databases
STRING	A7NE12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5523841.
GenomeReviews	Gene locus FTA_1739 in contig CP000803_GR.
KEGG	fta:FTA_1739.
PATRIC	17948928. VBIFraTul129798_1812.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	ADTIGMP.
ProtClustDB	CLSK934381.
Enzyme and pathway databases
BioCyc	FTUL458234:FTA_1739-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_FRATF

Accession

Primary (citable) accession number: A7NE12

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program