Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A7NB78 (TYSY_FRATF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:FTA_0755
OrganismFrancisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) [Complete proteome] [HAMAP]
Taxonomic identifier458234 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274Thymidylate synthase HAMAP-Rule MF_00008
PRO_1000000598

Sites

Active site1561 By similarity

Sequences

Sequence LengthMass (Da)Tools
A7NB78 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 2414889305960F98

FASTA27431,415
        10         20         30         40         50         60 
MREYLNFLKY IKENGVLKND RTGTGTRSIF GYQMRFDLQK GFPLVTTKKI HIPSVVHELL 

        70         80         90        100        110        120 
WFLSGSTNIK YLNDNNVRIW NEWATVDGEL GPIYGKQWRD FNGQGIDQIA DVIQMLKTNP 

       130        140        150        160        170        180 
NSRRILVLAW NPCVVPSEKI SPQENVVKGN SALPPCHAMF QFYVANNKLS CMLTQRSADA 

       190        200        210        220        230        240 
FLGVPFNIAS YSLLTHMVAQ QCNLDVGELI WSGGDCHIYN NHIEQVNEQL SREPLALPTL 

       250        260        270 
KILRKSNSIF DYKYEDFEFE NYNHHPAIKA KISV 

« Hide

References

[1]"Complete genome sequence of Francisella tularensis subspecies holarctica FTNF002-00."
Barabote R.D., Xie G., Brettin T.S., Hinrichs S.H., Fey P.D., Jay J.J., Engle J.L., Godbole S.D., Noronha J.M., Scheuermann R.H., Zhou L.W., Lion C., Dempsey M.P.
PLoS ONE 4:E7041-E7041(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FTNF002-00 / FTA.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000803 Genomic DNA. Translation: ABU61231.1.
RefSeqYP_001428187.1. NC_009749.1.

3D structure databases

ProteinModelPortalA7NB78.
SMRA7NB78. Positions 2-274.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING119857.FTL_0715.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU61231; ABU61231; FTA_0755.
GeneID5523355.
KEGGfta:FTA_0755.
PATRIC17946848. VBIFraTul129798_0792.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OMADVHLYSN.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

BioCycFTUL458234:GH31-717-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 2 hits.
ProtoNetSearch...

Entry information

Entry nameTYSY_FRATF
AccessionPrimary (citable) accession number: A7NB78
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways