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A7NAY4 (TRMB_FRATF) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:FTA_0661
OrganismFrancisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) [Complete proteome] [HAMAP]
Taxonomic identifier458234 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Protein attributes

Sequence length229 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 229229tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000064394

Regions

Region143 – 1486Interaction with RNA Potential
Region208 – 2114Substrate binding By similarity

Sites

Active site1371 By similarity
Binding site621S-adenosyl-L-methionine By similarity
Binding site871S-adenosyl-L-methionine By similarity
Binding site1141S-adenosyl-L-methionine By similarity
Binding site1371S-adenosyl-L-methionine By similarity
Binding site1411Substrate By similarity
Binding site1731Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A7NAY4 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 9B52D52645F6474F

FASTA22926,385
        10         20         30         40         50         60 
MCDKSKENLR QIKSYVQRAG RVTKKQQQAL DNYAAKYLIE YAKDRKLDFT EIFANTNDVV 

        70         80         90        100        110        120 
LEIGFGMGGS LVEMALANPA KNYLGIEVHK AGVGNILYEI EHQNIANLLV MSHDAVEILE 

       130        140        150        160        170        180 
NMIADQSLAS IQVYFPDPWH KKKHNKRRLV NQTNIDLFAK KLKVGGVFHY ASDWLPYAEE 

       190        200        210        220 
VLELLENDSK YRNLYSGFAP RPEWRPLTKF EKRGQNLDHP ISDILFEKI 

« Hide

References

[1]"Complete genome sequence of Francisella tularensis subspecies holarctica FTNF002-00."
Barabote R.D., Xie G., Brettin T.S., Hinrichs S.H., Fey P.D., Jay J.J., Engle J.L., Godbole S.D., Noronha J.M., Scheuermann R.H., Zhou L.W., Lion C., Dempsey M.P.
PLoS ONE 4:E7041-E7041(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FTNF002-00 / FTA.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000803 Genomic DNA. Translation: ABU61137.1.
RefSeqYP_001428093.1. NC_009749.1.

3D structure databases

ProteinModelPortalA7NAY4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING458234.FTA_0661.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU61137; ABU61137; FTA_0661.
GeneID5524262.
KEGGfta:FTA_0661.
PATRIC17946645. VBIFraTul129798_0695.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAHSTLEMA.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycFTUL458234:GH31-624-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_FRATF
AccessionPrimary (citable) accession number: A7NAY4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 2, 2007
Last modified: June 11, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways