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A7NAI2

- GLGB_FRATF

UniProt

A7NAI2 - GLGB_FRATF

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Protein
1,4-alpha-glucan branching enzyme GlgB
Gene
glgB, FTA_0509
Organism
Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei318 – 3181Nucleophile By similarity
Active sitei371 – 3711Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciFTUL458234:GH31-485-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:FTA_0509
OrganismiFrancisella tularensis subsp. holarctica (strain FTNF002-00 / FTA)
Taxonomic identifieri458234 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000000261: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6406401,4-alpha-glucan branching enzyme GlgBUniRule annotation
PRO_1000044977Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi458234.FTA_0509.

Structurei

3D structure databases

ProteinModelPortaliA7NAI2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiAKLLFMG.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

A7NAI2-1 [UniParc]FASTAAdd to Basket

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MKNKNSEQNT HYTIGEQDIH YFHEGKHIYA YEFMGAHKAC EEGIEGIRFT    50
TWAPNAKSIC VIGDFNYWQV EDKNYMEPIT DAGLWSVFIP NAKNGDKYKF 100
VVTNKDTSHY VYKSDPYAFF SELRPNTASI ITTETQYTWS DDKWLEKRAK 150
TNYYDNPMNV YELHLASWKT KNGKFLTYDE LSETLPQYIK EMGYTHVEFM 200
PLHEHPLDAS WGYQPTGFYS VNSRHGDIIG LKRLVDKLHN NDIGVILDWV 250
PGHFCKDQHG LIYFDGSPCY EYQEPTKAIN KGWETHNFDL GRNEVKCFLI 300
SNAMYWINEF HIDGLRVDAV SNILYLNYDR EDGQWIPNIY GGHENLEGIA 350
FLKELNGVLK HTCKGVITIA EESSSWPDIS TPVEKGGLGF DFKWNMGWMN 400
DTLRYISLDP VYRKYHHNLI TFSMVYHYSE KFILSISHDE VVHGKKSLIN 450
KMWGDLWNKY AGLRLYMSYM IGHPGKKLIF MGSEFVQFVE WREYEQLQWQ 500
VVDQYESHKQ TLHFFKKLND FYHNETALWQ CDYDHHGFRW IDANNSQQSI 550
LSFIRSSKDN KQKLIFICNF TPVTYYDYHL GVPDAGSYKE VFNSDNLEFG 600
GSGQVMATEI FSSPQSSHGF EQRITIKIPP MATLVLKLIK 640
Length:640
Mass (Da):74,910
Last modified:October 2, 2007 - v1
Checksum:i8A71BD846B60180E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000803 Genomic DNA. Translation: ABU60985.1.
RefSeqiYP_001427941.1. NC_009749.1.

Genome annotation databases

EnsemblBacteriaiABU60985; ABU60985; FTA_0509.
GeneIDi5522905.
KEGGifta:FTA_0509.
PATRICi17946318. VBIFraTul129798_0535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000803 Genomic DNA. Translation: ABU60985.1 .
RefSeqi YP_001427941.1. NC_009749.1.

3D structure databases

ProteinModelPortali A7NAI2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 458234.FTA_0509.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABU60985 ; ABU60985 ; FTA_0509 .
GeneIDi 5522905.
KEGGi fta:FTA_0509.
PATRICi 17946318. VBIFraTul129798_0535.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000283037.
KOi K00700.
OMAi AKLLFMG.
OrthoDBi EOG6JX7GT.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci FTUL458234:GH31-485-MONOMER.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FTNF002-00 / FTA.

Entry informationi

Entry nameiGLGB_FRATF
AccessioniPrimary (citable) accession number: A7NAI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: June 11, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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