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A7NAD2

- SYI_FRATF

UniProt

A7NAD2 - SYI_FRATF

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Protein

Isoleucine--tRNA ligase

Gene
ileS, FTA_0458
Organism
Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei558 – 5581Aminoacyl-adenylate By similarity
Binding sitei602 – 6021ATP By similarity
Metal bindingi897 – 8971Zinc By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi917 – 9171Zinc By similarity
Metal bindingi920 – 9201Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciFTUL458234:GH31-429-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:FTA_0458
OrganismiFrancisella tularensis subsp. holarctica (strain FTNF002-00 / FTA)
Taxonomic identifieri458234 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000000261: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 935935Isoleucine--tRNA ligaseUniRule annotationPRO_1000022066Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi458234.FTA_0458.

Structurei

3D structure databases

ProteinModelPortaliA7NAD2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi599 – 6035"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7NAD2-1 [UniParc]FASTAAdd to Basket

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MSDYKDTLNL PKTSFSMKGN LANKEPMILN KWEKQGIYKK IREHFAGREK    50
FVLHDGPPYA NGSIHVGHAV NKILKDIIIK SKTLSGYDAP FTPTWDCHGL 100
PIELQVEKKH GKAGQSISED DFRKECRKYA KKQVEIQKKD FKRLGVLGDW 150
EQPYLTMNFD YEANMIRTLA KIIENGHLSK GFKPVHWCTD CGSALAEAEV 200
EYADKVSPAI DVKFKIKDKD KLAQAFGLDS LNHDAFAIIW TTTPWTLPAN 250
QAIAVNNQLN YSLIKIEDFY IILAENLVEQ TLKRYAIENA QIIATTTGNK 300
LTGIIAEHPF YSRHVPILHG DHVTDDSGTG LVHTAPTHGV DDFTLGKEHN 350
LSMEIFVKGN GCYSENTKLF AGEFIFKASD RIIELLGEKK RLMNSDKIKH 400
SYPHCWRHKT PLMFRATPQW FISMEKQGLR DKALQTIKET SWAPSWGQAR 450
IEGMVKDRPD WCISRQRTWG VPLPLFIHKE TEELHPNTIE ILHKVAEKIE 500
KDGIEAWFNA DDCEFITETA QYKSVKDTLD VWFDSGSSSM CILDLDKRLS 550
YPADLYLEGS DQHRGWFQTS LLVAMSAKGS QPYKEVFTHG FVVDEHGRKM 600
SKSLGNVTSP QDIYNTLGAD ILRLWTASTD YKSEMAVSDQ ILKRTADTYR 650
RLRNTARFLL SNLDGFNPVT DIIEFDKLVK LDQWAIAKTK EFQDKIIEAY 700
DKYQTHTVAQ LIHHFCSIEM GSFYLDIIKD RQYTAKTDGH PRKSAQTAIY 750
HIVHALVRWM APILSFTADE IWDATPKTTD LPIQLCEWYT GLKSFDQDAE 800
LDLEYWAKIQ EIRSEVNRVL EIKRNEDVIK ASLEAEITIY ADKYNYKLLE 850
KLGNELRFLL ISSKADLKVI EESTSSSIAA NILGLLIEIT KIEEPKCERC 900
WHRSSTVGDN PQYKDICSRC VENITTEAGE SREFA 935
Length:935
Mass (Da):106,972
Last modified:October 2, 2007 - v1
Checksum:i356B45F79D494088
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000803 Genomic DNA. Translation: ABU60935.1.
RefSeqiWP_011457376.1. NC_009749.1.
YP_001427891.1. NC_009749.1.

Genome annotation databases

EnsemblBacteriaiABU60935; ABU60935; FTA_0458.
GeneIDi5524081.
KEGGifta:FTA_0458.
PATRICi17946198. VBIFraTul129798_0483.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000803 Genomic DNA. Translation: ABU60935.1 .
RefSeqi WP_011457376.1. NC_009749.1.
YP_001427891.1. NC_009749.1.

3D structure databases

ProteinModelPortali A7NAD2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 458234.FTA_0458.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABU60935 ; ABU60935 ; FTA_0458 .
GeneIDi 5524081.
KEGGi fta:FTA_0458.
PATRICi 17946198. VBIFraTul129798_0483.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci FTUL458234:GH31-429-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FTNF002-00 / FTA.

Entry informationi

Entry nameiSYI_FRATF
AccessioniPrimary (citable) accession number: A7NAD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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