CAI-1 autoinducer sensor kinase/phosphatase CqsS - Vibrio harveyi (strain ATCC BAA-1116 / BB120)

Preferred order of sections

Names and origin

Protein names

Recommended name:
CAI-1 autoinducer sensor kinase/phosphatase CqsS
EC=2.7.13.3
EC=3.1.3.-

Alternative name(s):
Cholerae quorum-sensing sensor

Gene names

Name:	cqsS
Ordered Locus Names:	VIBHAR_06089

Organism

Vibrio harveyi (strain ATCC BAA-1116 / BB120) [Complete proteome] [HAMAP]

Taxonomic identifier

338187 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	681 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Senses the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal. The sensory signal is then relayed to luxU and luxO. Ref.1
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 681

681

CAI-1 autoinducer sensor kinase/phosphatase CqsS

PRO_0000316892

Regions

Transmembrane

17 – 37

21

Helical; Potential

Transmembrane

73 – 93

21

Helical; Potential

Transmembrane

112 – 132

21

Helical; Potential

Transmembrane

148 – 168

21

Helical; Potential

Domain

187 – 413

227

Histidine kinase

Domain

564 – 681

118

Response regulatory

Amino acid modifications

Modified residue

190

1

Phosphohistidine; by autocatalysis By similarity

Modified residue

613

1

4-aspartylphosphate By similarity

Experimental info

Sequence conflict

484

1

A → R in AAT86007. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

A7N6S2 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 66EFACCF3F3DF932
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FASTA

681

77,799

        10         20         30         40         50         60 
MDAIRKVYQY AEPNLSLVGW MGFIGFPIYY IVWEFMFPQP YENLPLRILC SVLFFGIIYR 

        70         80         90        100        110        120 
NRTPFEWRGF LPAYYQVVTT LCLPCFFFYM LLMNNWSNVW VMSFMSAIFL HILLVHITSV 

       130        140        150        160        170        180 
MFVQTFVGIG LATFFAWVAQ GFHLELTMDW THVPIFLFIY LFGNLFYFRN QVEHEAKVSI 

       190        200        210        220        230        240 
AKSFGAGIAH EMRNPLSGLL TSIDVIQSVL PNPKEGKKEQ YTLSDEDVTL LREVSSDAMK 

       250        260        270        280        290        300 
IIHSGNETID LLLTSIDENR VSRSTFKKHS AQSVVESAIE SFSYKRSTDR FAISLDVRSE 

       310        320        330        340        350        360 
FDFLGSDTLL KYVMYNLFKN AFHHRSSEDF HIHVTMYSDE FANQIVVTDN GSGIAPEVLQ 

       370        380        390        400        410        420 
SIFQDFYTTG KSGNYGLGLP FCKKVMRSFG GDIRCQSEVG EWSQFTMTFP TIGSSAVKEI 

       430        440        450        460        470        480 
KSELTKLKTI LFVSEQNILV SKVTDIARFM RFELTVLDVP AVLKNKEYEF EFDLILIDME 

       490        500        510        520        530        540 
SLDASGSHID KVESLLSFTE ARIVYMFEHH PIQRARSVSF EPIWVETQAW LLNTRATIDR 

       550        560        570        580        590        600 
LLYDANYVVP SMPAKPLDST NKRTIMVVDD NESLRKFTAM LLEKQGFEVI QTEDGLQAIN 

       610        620        630        640        650        660 
ALNENNVDLI LMDIEMPVMD GVEASRQIRG SNKAYASVPI IAHTGDSSPI TLDKIGSSGM 

       670        680 
SDFIVKPADK NRLFDKIANW I

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three parallel quorum-sensing systems regulate gene expression in Vibrio harveyi." Henke J.M., Bassler B.L. J. Bacteriol. 186:6902-6914(2004) [PubMed: 15466044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS THE CAI-1 SENSOR.
[2]	The Vibrio harveyi Genome Sequencing Project Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C., Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N., Pepin K., Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C., Irgon J., Wilson R.K. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1116 / BB120.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY625893 Genomic DNA. Translation: AAT86007.1. CP000790 Genomic DNA. Translation: ABU73982.1.
RefSeq	YP_001448209.1. NC_009784.1.
3D structure databases
ProteinModelPortal	A7N6S2.
ModBase	Search...
Protein-protein interaction databases
STRING	A7N6S2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5556966.
GenomeReviews	Gene locus VIBHAR_06089 in contig CP000790_GR.
KEGG	vha:VIBHAR_06089.
NMPDR	fig\|338187.4.peg.3584.
PATRIC	20136459. VBIVibHar24526_4709.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0642.
HOGENOM	HBG625184.
OMA	AIFLHIL.
ProtClustDB	CLSK869633.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011006. CheY-like_superfamily. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
KO	K10916.
Pfam	PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF52172. CheY_like. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CQSS_VIBHB

Accession

Primary (citable) accession number: A7N6S2
Secondary accession number(s): Q693Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents