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A7N512 (DDL_VIBCB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:VIBHAR_05785
OrganismVibrio campbellii (strain ATCC BAA-1116 / BB120) [Complete proteome] [HAMAP]
Taxonomic identifier338187 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030519

Regions

Domain120 – 326207ATP-grasp
Nucleotide binding150 – 20556ATP By similarity

Sites

Metal binding2801Magnesium or manganese 1 By similarity
Metal binding2931Magnesium or manganese 1 By similarity
Metal binding2931Magnesium or manganese 2 By similarity
Metal binding2951Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A7N512 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: B8C2ADAAAEA3DB70

FASTA32936,657
        10         20         30         40         50         60 
MIKNILLLCG GGSSEHEISL LSANFVEQQL NLIENVSVTR VEIKNEGWVT NQGELVYLDP 

        70         80         90        100        110        120 
NQKKLCSDNL SLDVDFIVPC IHGFPGETGD IQSMFEIAGI PYLGCGPEAS SNSFNKITSK 

       130        140        150        160        170        180 
LWYDAIGIPN TPYLFLTRND EQAHEQALQA FDKWGKVFVK AARQGSSVGC YSVTNKQSVS 

       190        200        210        220        230        240 
QAVNDAFGYS EQVLVEKSVK PRELEVAAYE MNGELHITKP GEVIAPDGAF YSYDEKYSTS 

       250        260        270        280        290        300 
SHSLTEVEAK NLTEEQVEAI RVASETVFKQ MNLRHLSRID FFLTEDGEIY LNEVNTFPGM 

       310        320 
TPISMFPKML QNNGHKFHEF LADCINTAI 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000790 Genomic DNA. Translation: ABU73679.1.
RefSeqYP_001447906.1. NC_009784.1.
YP_008529108.1. NC_022270.1.

3D structure databases

ProteinModelPortalA7N512.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING338187.VIBHAR_05785.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU73679; ABU73679; VIBHAR_05785.
GeneID16818671.
5557281.
KEGGvca:M892_22760.
vha:VIBHAR_05785.
PATRIC20135940. VBIVibHar24526_4450.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycVHAR338187:GJCH-4771-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_VIBCB
AccessionPrimary (citable) accession number: A7N512
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways