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A7N1X9

- GLND_VIBCB

UniProt

A7N1X9 - GLND_VIBCB

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Vibrio campbellii (strain ATCC BAA-1116 / BB120)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciVHAR338187:GJCH-3229-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:VIBHAR_03239
OrganismiVibrio campbellii (strain ATCC BAA-1116 / BB120)
Taxonomic identifieri338187 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000008152: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 874874Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000022355Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi338187.VIBHAR_03239.

Structurei

3D structure databases

ProteinModelPortaliA7N1X9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini452 – 584133HDUniRule annotationAdd
BLAST
Domaini693 – 77785ACT 1UniRule annotationAdd
BLAST
Domaini800 – 87475ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 332332UridylyltransferaseAdd
BLAST
Regioni333 – 692360Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7N1X9 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLQSPLTFR DEQINIGELK QELEKFSSYQ KEEFLQHHPV TNLVLSRAEY
60 70 80 90 100
MDLLLNRLWQ HFGFKDIHNI SLVAVGGYGR GELHPLSDID ILILSNNKLP
110 120 130 140 150
NALEAKISEF ITLLWDLRLE VGHAVRTVDE CAEIGRADLT VATNLQEARL
160 170 180 190 200
LCGSEDTFQA LKKVVLSDSF WPSETFYRAK IQEQRERHAR YHDTTYNLEP
210 220 230 240 250
DIKSTPGGLR DIHTLSWVAR RHFGATSLLE MSRYGFLTDA EYRELVECQD
260 270 280 290 300
ILWRVRFALH IELRRYDNRL TFAHQAQVAE HLGYVGEGNR GVEMMMKEFY
310 320 330 340 350
RTLRRVAELN KMLLKLFDQA IINGGASEDA EIIDEDFQRR GALIEARKPA
360 370 380 390 400
LFQARPETIL DMFLHIANDS SIEGVSPPTL RQLRTARRRL NKFLHTIPEA
410 420 430 440 450
REKFMALCRH PNALHKAFSL MHKLGVLAAY LPQWSQIVGQ MQFDLFHVYT
460 470 480 490 500
VDEHSIRLLK HINTFSYAEN HSKHPICCEV YPRIQKKELL ILAAIFHDIG
510 520 530 540 550
KGRGGDHSVI GEGEAYDFCI EHGLSKPEAK LVSWLVRHHL LMSVTAQRRD
560 570 580 590 600
IYDPDVITEF AKQVRDEEYL EYLVCLTVAD ICATNPELWN SWKRTLLAEL
610 620 630 640 650
FYSTQRALRR GLENPVDVRE RIRHNQQMAS AQLRKEGFSA REIEVLWQRF
660 670 680 690 700
KADYFLRHTH KQIAWHCENL LRMEDTSKPL VLISKKATRG GTEVFVYSPD
710 720 730 740 750
QPALFATVVA ELDRRNFNVH DAQIMTSKDG YVLDTFMVLD QHGKAIEEGR
760 770 780 790 800
HSAVTKHITH VLEDGRPTKI KTRRTPNKLQ HFNVKTKVDF LPTKSKKRTL
810 820 830 840 850
MEFVALDTPG LLAKVGRTFA DLGINLHAAK ITTIGERAED LFILTSEAGG
860 870
RLSEEQQTEL REKLIEKLSD SVSA
Length:874
Mass (Da):100,710
Last modified:October 2, 2007 - v1
Checksum:iC615AE9FF2E84D6E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000789 Genomic DNA. Translation: ABU72188.1.
RefSeqiWP_012128699.1. NC_022269.1.
YP_001446415.1. NC_009783.1.
YP_008527642.1. NC_022269.1.

Genome annotation databases

EnsemblBacteriaiABU72188; ABU72188; VIBHAR_03239.
GeneIDi16817140.
5556041.
KEGGivca:M892_14880.
vha:VIBHAR_03239.
PATRICi20133113. VBIVibHar24526_3086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000789 Genomic DNA. Translation: ABU72188.1 .
RefSeqi WP_012128699.1. NC_022269.1.
YP_001446415.1. NC_009783.1.
YP_008527642.1. NC_022269.1.

3D structure databases

ProteinModelPortali A7N1X9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 338187.VIBHAR_03239.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABU72188 ; ABU72188 ; VIBHAR_03239 .
GeneIDi 16817140.
5556041.
KEGGi vca:M892_14880.
vha:VIBHAR_03239.
PATRICi 20133113. VBIVibHar24526_3086.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci VHAR338187:GJCH-3229-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1116 / BB120.

Entry informationi

Entry nameiGLND_VIBCB
AccessioniPrimary (citable) accession number: A7N1X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3