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A7N1X9

- GLND_VIBCB

UniProt

A7N1X9 - GLND_VIBCB

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Vibrio campbellii (strain ATCC BAA-1116 / BB120)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciVHAR338187:GJCH-3229-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:VIBHAR_03239
    OrganismiVibrio campbellii (strain ATCC BAA-1116 / BB120)
    Taxonomic identifieri338187 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000008152: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 874874Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000022355Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi338187.VIBHAR_03239.

    Structurei

    3D structure databases

    ProteinModelPortaliA7N1X9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini452 – 584133HDUniRule annotationAdd
    BLAST
    Domaini693 – 77785ACT 1UniRule annotationAdd
    BLAST
    Domaini800 – 87475ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 332332UridylyltransferaseAdd
    BLAST
    Regioni333 – 692360Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7N1X9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLQSPLTFR DEQINIGELK QELEKFSSYQ KEEFLQHHPV TNLVLSRAEY    50
    MDLLLNRLWQ HFGFKDIHNI SLVAVGGYGR GELHPLSDID ILILSNNKLP 100
    NALEAKISEF ITLLWDLRLE VGHAVRTVDE CAEIGRADLT VATNLQEARL 150
    LCGSEDTFQA LKKVVLSDSF WPSETFYRAK IQEQRERHAR YHDTTYNLEP 200
    DIKSTPGGLR DIHTLSWVAR RHFGATSLLE MSRYGFLTDA EYRELVECQD 250
    ILWRVRFALH IELRRYDNRL TFAHQAQVAE HLGYVGEGNR GVEMMMKEFY 300
    RTLRRVAELN KMLLKLFDQA IINGGASEDA EIIDEDFQRR GALIEARKPA 350
    LFQARPETIL DMFLHIANDS SIEGVSPPTL RQLRTARRRL NKFLHTIPEA 400
    REKFMALCRH PNALHKAFSL MHKLGVLAAY LPQWSQIVGQ MQFDLFHVYT 450
    VDEHSIRLLK HINTFSYAEN HSKHPICCEV YPRIQKKELL ILAAIFHDIG 500
    KGRGGDHSVI GEGEAYDFCI EHGLSKPEAK LVSWLVRHHL LMSVTAQRRD 550
    IYDPDVITEF AKQVRDEEYL EYLVCLTVAD ICATNPELWN SWKRTLLAEL 600
    FYSTQRALRR GLENPVDVRE RIRHNQQMAS AQLRKEGFSA REIEVLWQRF 650
    KADYFLRHTH KQIAWHCENL LRMEDTSKPL VLISKKATRG GTEVFVYSPD 700
    QPALFATVVA ELDRRNFNVH DAQIMTSKDG YVLDTFMVLD QHGKAIEEGR 750
    HSAVTKHITH VLEDGRPTKI KTRRTPNKLQ HFNVKTKVDF LPTKSKKRTL 800
    MEFVALDTPG LLAKVGRTFA DLGINLHAAK ITTIGERAED LFILTSEAGG 850
    RLSEEQQTEL REKLIEKLSD SVSA 874
    Length:874
    Mass (Da):100,710
    Last modified:October 2, 2007 - v1
    Checksum:iC615AE9FF2E84D6E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000789 Genomic DNA. Translation: ABU72188.1.
    RefSeqiYP_001446415.1. NC_009783.1.
    YP_008527642.1. NC_022269.1.

    Genome annotation databases

    EnsemblBacteriaiABU72188; ABU72188; VIBHAR_03239.
    GeneIDi16817140.
    5556041.
    KEGGivca:M892_14880.
    vha:VIBHAR_03239.
    PATRICi20133113. VBIVibHar24526_3086.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000789 Genomic DNA. Translation: ABU72188.1 .
    RefSeqi YP_001446415.1. NC_009783.1.
    YP_008527642.1. NC_022269.1.

    3D structure databases

    ProteinModelPortali A7N1X9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 338187.VIBHAR_03239.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABU72188 ; ABU72188 ; VIBHAR_03239 .
    GeneIDi 16817140.
    5556041.
    KEGGi vca:M892_14880.
    vha:VIBHAR_03239.
    PATRICi 20133113. VBIVibHar24526_3086.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci VHAR338187:GJCH-3229-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1116 / BB120.

    Entry informationi

    Entry nameiGLND_VIBCB
    AccessioniPrimary (citable) accession number: A7N1X9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3