ID   FADB_VIBHB              Reviewed;         723 AA.
AC   A7N1D2;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   16-JUN-2009, entry version 16.
DE   RecName: Full=Fatty acid oxidation complex subunit alpha;
DE   Includes:
DE     RecName: Full=Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase;
DE              EC=4.2.1.17;
DE              EC=5.3.3.8;
DE              EC=5.1.2.3;
DE   Includes:
DE     RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
DE              EC=1.1.1.35;
GN   Name=fadB; OrderedLocusNames=VIBHAR_00460;
OS   Vibrio harveyi (strain ATCC BAA-1116 / BB120).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=338187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C.,
RA   Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N.,
RA   Pepin K., Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C.,
RA   Irgon J., Wilson R.K.;
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of an hydroxyacyl-CoA by
CC       addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA
CC       epimerase and 3-hydroxyacyl-CoA dehydrogenase activities (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA
CC       + NADH.
CC   -!- CATALYTIC ACTIVITY: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-
CC       CoA + H(2)O.
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxybutanoyl-CoA = (R)-3-
CC       hydroxybutanoyl-CoA.
CC   -!- CATALYTIC ACTIVITY: (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-
CC       CoA.
CC   -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC   -!- SUBUNIT: Heterotetramer of two alpha chains (fadB) and two beta
CC       chains (fadA) (By similarity).
CC   -!- SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA
CC       hydratase/isomerase family.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the 3-
CC       hydroxyacyl-CoA dehydrogenase family.
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DR   EMBL; CP000789; ABU69475.1; -; Genomic_DNA.
DR   RefSeq; YP_001443702.1; -.
DR   GeneID; 5552832; -.
DR   GenomeReviews; CP000789_GR; VIBHAR_00460.
DR   KEGG; vha:VIBHAR_00460; -.
DR   NMPDR; fig|338187.4.peg.356; -.
DR   OMA; A7N1D2; QFANSIF.
DR   GO; GO:0016507; C:fatty acid beta-oxidation multienzyme complex; IEA:InterPro.
DR   GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0008692; F:3-hydroxybutyryl-CoA epimerase activity; IEA:HAMAP.
DR   GO; GO:0050662; F:coenzyme binding; IEA:InterPro.
DR   GO; GO:0004165; F:dodecenoyl-CoA delta-isomerase activity; IEA:HAMAP.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:HAMAP.
DR   GO; GO:0009062; P:fatty acid catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01621; -; 1.
DR   InterPro; IPR006180; 3-OHacyl-CoA_DH_CS.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR001753; Crotonase_core.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR012799; FadB.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   Pfam; PF00725; 3HCDH; 1.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   Pfam; PF00378; ECH; 1.
DR   TIGRFAMs; TIGR02437; FadB; 1.
DR   PROSITE; PS00067; 3HCDH; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; FALSE_NEG.
PE   3: Inferred from homology;
KW   Complete proteome; Fatty acid metabolism; Isomerase;
KW   Lipid degradation; Lipid metabolism; Lyase; Multifunctional enzyme;
KW   NAD; Oxidoreductase.
FT   CHAIN         1    723       Fatty acid oxidation complex subunit
FT                                alpha.
FT                                /FTId=PRO_1000069583.
FT   REGION        1    189       Enoyl-CoA hydratase/isomerase (By
FT                                similarity).
FT   REGION      312    723       3-hydroxyacyl-CoA dehydrogenase (By
FT                                similarity).
SQ   SEQUENCE   723 AA;  78649 MW;  F9A6F1B919EF479D CRC64;
     MIYQAETLQV KEVQDGIAEL SFCSPKSVNK LDLATLESLD KALDALKAHQ GLKGLMLTSD
     KDAFIVGADI TEFLGLFAKT DAELDQWLQF ANSIFNKLED LPVPTISVLK GHTLGGGCEC
     VLATDMRIGD KTTSIGLPET KLGIMPGFGG CVRLPRVIGA DSAMEVITQG KACRADEALK
     IGLLDAVVDS DALYESALKT LTSAINEKLD WQARRKQKTS PLTLSKLESM MSFTMAKGLV
     AQKAGPHYPA PMTAVITIEE GARFARNEAL DIERKHFVKL AKSEEAKSLV GLFLNDQYIK
     GLAKKSAKSA SKDTQRAAVL GAGIMGGGIA YQSALKGVPV LMKDIAQPSL DLGMTEASKL
     LNKRLSRGRI DGFKMAGILA SITPSLHYTG IEESDVIVEA VVENPKVKAA VLSEVESHVG
     EDTVITSNTS TIPINLLAKS LKRPENFCGM HFFNPVHRMP LVEIIRGEHT SDETINRVVA
     YAAKMGKSPI VVNDCPGFFV NRVLFPYFGG FSMLLRDGAD FTKIDKIMER KFGWPMGPAY
     LLDVVGIDTA HHAQAVMAQG FPERMGKQGR DAIDALFEAS KYGQKNGSGF YSYTIDRKGK
     PKKTFSEEIL PVLADVCTDK QDFDDQTIIQ RMMIPMINEV VLCLQEGIIA TPQEADMTLV
     YGLGFPPFRG GVFRYLDSVG IAEFVAMAKQ YSELGAMYQV PQMLIDMAAK GESFYGAQQQ
     GSI
//