ID   MAO1_VIBHB              Reviewed;         562 AA.
AC   A7N025;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   16-JUN-2009, entry version 16.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=VIBHAR_02034;
OS   Vibrio harveyi (strain ATCC BAA-1116 / BB120).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=338187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C.,
RA   Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N.,
RA   Pepin K., Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C.,
RA   Irgon J., Wilson R.K.;
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; CP000789; ABU70999.1; -; Genomic_DNA.
DR   RefSeq; YP_001445226.1; -.
DR   GeneID; 5552921; -.
DR   GenomeReviews; CP000789_GR; VIBHAR_02034.
DR   KEGG; vha:VIBHAR_02034; -.
DR   NMPDR; fig|338187.4.peg.1515; -.
DR   OMA; A7N025; IQDNNET.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    562       NAD-dependent malic enzyme.
FT                                /FTId=PRO_1000069551.
FT   ACT_SITE    101    101       Proton donor (By similarity).
FT   ACT_SITE    172    172       Proton acceptor (By similarity).
FT   METAL       243    243       Divalent metal cation (By similarity).
FT   METAL       244    244       Divalent metal cation (By similarity).
FT   METAL       267    267       Divalent metal cation (By similarity).
FT   BINDING     154    154       NAD (By similarity).
FT   BINDING     267    267       NAD (By similarity).
FT   BINDING     415    415       NAD (By similarity).
FT   SITE        267    267       Important for activity (By similarity).
SQ   SEQUENCE   562 AA;  62260 MW;  8ECB19BE34870BE5 CRC64;
     MNNDKRPLYI PYAGPALLAT PLLNKGSAFS GEERSSFNLE GLLPETTETI QEQVERAYQQ
     YKSFESDMDK HIYLRNIQDT NETLYYRLVQ NHISEMMPII YTPTVGAACE NFSNIYRRGR
     GLFISYPNRD RIDDLLNNAA NHNVKVIVVT DGERILGLGD QGIGGMGIPI GKLSLYTACG
     GISPAYTLPI VLDVGTNNPQ RLADPMYMGW RHPRITGPDY DAFVEEFIQA VQRRWPDALI
     QFEDFAQKNA MPLLERYKDR ICCFNDDIQG TAAVTVGSLL AACKAAGTKL SDQRITFLGA
     GSAGCGIAEA IIAQMVSEGI SDEKARSQVY MVDRWGLLQE GMPNLLDFQQ RLVQKFDNTK
     KWENEGNGFS LLDVMHNAKP TILIGVSGAP GLFSEEVIKE MHKHCKRPIV FPLSNPTSRV
     EATPNDIIRW TNGEALVATG SPFEPVVHEG RSYPIAQCNN SYIFPGIGLG VLAVNAKRVT
     DEMLMESSRA LATCSPLAIN GNGALLPPLE EIHLVSKKIA FAVAKKAIEQ GVALEITDEA
     LNDAIDQAFW QPVYRRYKRT AF
//