A7MZ01 (KATG_VIBHB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	VIBHAR_01773

Organism

Vibrio harveyi (strain ATCC BAA-1116 / BB120) [Complete proteome] [HAMAP]

Taxonomic identifier

338187 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 726	726	Catalase-peroxidase HAMAP MF_01961		PRO_0000354949
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	265	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 224		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-250) By similarity
	Cross-link	224 ↔ 250		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MZ01 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: B9329E51C0E17E3B	FASTA	726	81,628
	10 20 30 40 50 60 MSDKNHHSKC PVIHGANTNQ QASEFNWWPK SLNLEILHQH DHKTDPMGAD FNYADAFNSL 70 80 90 100 110 120 DFEEVKKDLK DLMTDSQDWW PADWGHYGGL MIRMAWHSAG TYRIADGRGG ASRGNQRFAP 130 140 150 160 170 180 LNSWPDNGNL DKARRLLWPI KRKYGNKLSW ADLMILAGNM AYESMGFKTF GFAGGREDIW 190 200 210 220 230 240 HPEKDIYWGA EKEWLQPSGG ENNRYSGERD LENPLAAVMM GLIYVNPEGV DGKPDPLKTA 250 260 270 280 290 300 HDMRVTFARM AMNDEETVAL TAGGHTVGKA HGNGDASVLE PEPEGAEIED QGFGWLNKTS 310 320 330 340 350 360 RGIGRDTVTS GVEGAWTTHP TKWDNGYFHL LFTYDWEITK SPAGAAQWEP IDIKEEDMPV 370 380 390 400 410 420 DVEDSSIRHN PMMTDADMAL KYDPEYRKIA ERFRDNPKEF EDCFARAWFK LTHRDLGPKS 430 440 450 460 470 480 RYLGPDVPAE DLIWQDPVPP VSYYLSEDNI DALKMAILNS ELSIAELVST AWDSARTYRG 490 500 510 520 530 540 SDRRGGANGA RIRLAPQKDW VGNEPERLQK VLNVLDEIRT MHHSPISMAD LIVLAGGVGI 550 560 570 580 590 600 EKAASNAGMN ISVPFSQGRG DATDVMTDAE SFDVLEPIHD GFRNWLKSDY VVTAEELLLE 610 620 630 640 650 660 RSQLMQLTAV EMTVLIGGMR VLGTNYGGTS QGVFTDNVGA LTNDFFVHLT DMSYTWHPAG 670 680 690 700 710 720 DNQYEIRERA SGQTKWTASR VDLVFGSNSV LRSYAEVYAQ DDNKEKFVQD FVAAWVKVMN NDRFDL

References

[1]

The Vibrio harveyi Genome Sequencing Project Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C., Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N., Pepin K., Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C., Irgon J., Wilson R.K. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1116 / BB120.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000789 Genomic DNA. Translation: ABU70742.1.
RefSeq	YP_001444969.1. NC_009783.1.
3D structure databases
ProteinModelPortal	A7MZ01.
SMR	A7MZ01. Positions 23-726.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MZ01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5554168.
GenomeReviews	Gene locus VIBHAR_01773 in contig CP000789_GR.
KEGG	vha:VIBHAR_01773.
NMPDR	fig|338187.4.peg.1338.
PATRIC	20130319. VBIVibHar24526_1701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_VIBHB

Accession

Primary (citable) accession number: A7MZ01

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program