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A7MY86 (SYL_VIBCB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:VIBHAR_01222
OrganismVibrio campbellii (strain ATCC BAA-1116 / BB120) [Complete proteome] [HAMAP]
Taxonomic identifier338187 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length862 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

regulation of translational fidelity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 862862Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009463

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7MY86 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 2C3CDC8621D78232

FASTA86297,056
        10         20         30         40         50         60 
MQEQYNPQDL EQKVQKHWDD NKTFVVSEDP NKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVSRFQRLQG KNVMQPIGWD AFGLPAENAA VKNNTAPAPW TYENIEYMKN QLKLLGFGYD 

       130        140        150        160        170        180 
WNREFATCTP EYYRWEQEFF TKLYEKGLVY KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD 

       190        200        210        220        230        240 
TPVEQKEIPQ WFIKITEYAQ ELLDDLDKLE GWPEMVKTMQ RNWIGRSEGV ELRFALKDSE 

       250        260        270        280        290        300 
VKGQQDLEVY TTRPDTLMGV TYVGIAAGHP LATIAAENNP ELAAFIEECK NTKVAEAELA 

       310        320        330        340        350        360 
TMEKKGMATG LTAIHPLNGR EVPVYVANFV LMDYGTGAVM AVPAHDQRDF EFATKYGLDI 

       370        380        390        400        410        420 
VPVIKPVDGS ELDTSEAAYT EKGVLFDSGE FDGLEFQAAF DAIAAKLEAE GKGTKTVNFR 

       430        440        450        460        470        480 
LRDWGVSRQR YWGAPIPMVT TEDGEVHPVP ADQLPVILPE DVVMDGVTSP IKADKEWAKT 

       490        500        510        520        530        540 
TFNGEPALRE TDTFDTFMES SWYYARYCSP QADDILAPEK ANYWLPVDQY IGGIEHACMH 

       550        560        570        580        590        600 
LLYSRFFHKL LRDAGYVTSD EPFKQLLCQG MVLADAFYFE NEKGGKEWVA PTDVAVERDG 

       610        620        630        640        650        660 
KGRITSAKDT EGRDVTHSGM IKMSKSKNNG IDPQEMVDKY GADTVRLFMM FASPADMTLE 

       670        680        690        700        710        720 
WQESGVEGAN RFLKRVWKLV NEHTSKGAAE AVDAAALSGD QKALRRDVHK TIAKVTDDID 

       730        740        750        760        770        780 
RRQTFNTAIA AIMELMNKLA KAPQESAQDR VILDEALKAV VAMLYPITPH ISYELWAALG 

       790        800        810        820        830        840 
EADIDNAAWP TFDEKALVED EKTIVVQVNG KLRAKLTVAA DATKEQVEEL GLNDENVTKF 

       850        860 
TDGLTIRKVI YVPGKLLNIV AN 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000789 Genomic DNA. Translation: ABU70211.1.
RefSeqYP_001444438.1. NC_009783.1.
YP_008526138.1. NC_022269.1.

3D structure databases

ProteinModelPortalA7MY86.
SMRA7MY86. Positions 228-417.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING338187.VIBHAR_01222.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU70211; ABU70211; VIBHAR_01222.
GeneID16815541.
5554910.
KEGGvha:VIBHAR_01222.
PATRIC20129277. VBIVibHar24526_1199.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycVHAR338187:GJCH-1213-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_VIBCB
AccessionPrimary (citable) accession number: A7MY86
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: February 19, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries