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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Vibrio campbellii (strain ATCC BAA-1116 / BB120)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei625 – 6251ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVHAR338187:GJCH-1213-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:VIBHAR_01222
OrganismiVibrio campbellii (strain ATCC BAA-1116 / BB120)
Taxonomic identifieri338187 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000008152 Componenti: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 862862Leucine--tRNA ligasePRO_1000009463Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi338187.VIBHAR_01222.

Structurei

3D structure databases

SMRiA7MY86. Positions 228-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi622 – 6265"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MY86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQYNPQDL EQKVQKHWDD NKTFVVSEDP NKEKFYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VVSRFQRLQG KNVMQPIGWD AFGLPAENAA VKNNTAPAPW
110 120 130 140 150
TYENIEYMKN QLKLLGFGYD WNREFATCTP EYYRWEQEFF TKLYEKGLVY
160 170 180 190 200
KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD TPVEQKEIPQ WFIKITEYAQ
210 220 230 240 250
ELLDDLDKLE GWPEMVKTMQ RNWIGRSEGV ELRFALKDSE VKGQQDLEVY
260 270 280 290 300
TTRPDTLMGV TYVGIAAGHP LATIAAENNP ELAAFIEECK NTKVAEAELA
310 320 330 340 350
TMEKKGMATG LTAIHPLNGR EVPVYVANFV LMDYGTGAVM AVPAHDQRDF
360 370 380 390 400
EFATKYGLDI VPVIKPVDGS ELDTSEAAYT EKGVLFDSGE FDGLEFQAAF
410 420 430 440 450
DAIAAKLEAE GKGTKTVNFR LRDWGVSRQR YWGAPIPMVT TEDGEVHPVP
460 470 480 490 500
ADQLPVILPE DVVMDGVTSP IKADKEWAKT TFNGEPALRE TDTFDTFMES
510 520 530 540 550
SWYYARYCSP QADDILAPEK ANYWLPVDQY IGGIEHACMH LLYSRFFHKL
560 570 580 590 600
LRDAGYVTSD EPFKQLLCQG MVLADAFYFE NEKGGKEWVA PTDVAVERDG
610 620 630 640 650
KGRITSAKDT EGRDVTHSGM IKMSKSKNNG IDPQEMVDKY GADTVRLFMM
660 670 680 690 700
FASPADMTLE WQESGVEGAN RFLKRVWKLV NEHTSKGAAE AVDAAALSGD
710 720 730 740 750
QKALRRDVHK TIAKVTDDID RRQTFNTAIA AIMELMNKLA KAPQESAQDR
760 770 780 790 800
VILDEALKAV VAMLYPITPH ISYELWAALG EADIDNAAWP TFDEKALVED
810 820 830 840 850
EKTIVVQVNG KLRAKLTVAA DATKEQVEEL GLNDENVTKF TDGLTIRKVI
860
YVPGKLLNIV AN
Length:862
Mass (Da):97,056
Last modified:October 1, 2007 - v1
Checksum:i2C3CDC8621D78232
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000789 Genomic DNA. Translation: ABU70211.1.
RefSeqiWP_012127189.1. NC_022269.1.
YP_001444438.1. NC_009783.1.
YP_008526138.1. NC_022269.1.

Genome annotation databases

EnsemblBacteriaiABU70211; ABU70211; VIBHAR_01222.
GeneIDi5554910.
KEGGivca:M892_06855.
vha:VIBHAR_01222.
PATRICi20129277. VBIVibHar24526_1199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000789 Genomic DNA. Translation: ABU70211.1.
RefSeqiWP_012127189.1. NC_022269.1.
YP_001444438.1. NC_009783.1.
YP_008526138.1. NC_022269.1.

3D structure databases

SMRiA7MY86. Positions 228-417.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi338187.VIBHAR_01222.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU70211; ABU70211; VIBHAR_01222.
GeneIDi5554910.
KEGGivca:M892_06855.
vha:VIBHAR_01222.
PATRICi20129277. VBIVibHar24526_1199.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciVHAR338187:GJCH-1213-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1116 / BB120.

Entry informationi

Entry nameiSYL_VIBCB
AccessioniPrimary (citable) accession number: A7MY86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 14, 2008
Last sequence update: October 1, 2007
Last modified: March 31, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.