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A7MY61 (SYE_VIBCB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:VIBHAR_01271
OrganismVibrio campbellii (strain ATCC BAA-1116 / BB120) [Complete proteome] [HAMAP]
Taxonomic identifier338187 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 475475Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_1000001983

Regions

Motif9 – 1911"HIGH" region HAMAP-Rule MF_00022
Motif240 – 2445"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2431ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7MY61 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: E2DC8F4AAE3110B2

FASTA47553,290
        10         20         30         40         50         60 
MTVKTRFAPS PTGYLHVGGA RTALYSWLYA KNQGGEFVLR IEDTDLERNS QEAVDAILEG 

        70         80         90        100        110        120 
MEWLGLEWNE GPYFQTQRFD RYNEMVDKLL EEDKAYKCYA SKELLDEVRA EQEANKEMPR 

       130        140        150        160        170        180 
YDANHPKIKA ANEAAKDGEP CVIRFRNPKE GSVVFDDQIR GRIEISNTQM DDLIIRRTDG 

       190        200        210        220        230        240 
SPTYNFCVVV DDWDMGITHV VRGEDHINNT PRQINIYEAL GAPVPTFAHC AMILGDDGAK 

       250        260        270        280        290        300 
LSKRHGAVSV MQYRDMGYLP AALNNYLVRL GWSHGDQEIF TQEEMINLFS LNAVSKSASA 

       310        320        330        340        350        360 
FNTDKLQWLN NHYIKNSDPA YVAEHLQWHL DQQKLDVTNG PAITEVIKLV GERCNTLVEL 

       370        380        390        400        410        420 
AEQIGYFYED FAEFEAGAAK KHLRGVAKEP LELALAKAEA LTEWTTANIK DGVIAAVCEE 

       430        440        450        460        470 
LEIGMGKIGM PLRVAVTGGG QSPSVDAVME LIGKERCVAR IKMALEFIAE REANA 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000789 Genomic DNA. Translation: ABU70249.1.
RefSeqYP_001444476.1. NC_009783.1.
YP_008526104.1. NC_022269.1.

3D structure databases

ProteinModelPortalA7MY61.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING338187.VIBHAR_01271.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU70249; ABU70249; VIBHAR_01271.
GeneID16815502.
5552812.
KEGGvca:M892_06655.
vha:VIBHAR_01271.
PATRIC20129370. VBIVibHar24526_1234.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252722.
KOK01885.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycVHAR338187:GJCH-1261-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_VIBCB
AccessionPrimary (citable) accession number: A7MY61
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries