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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Vibrio campbellii (strain ATCC BAA-1116 / BB120)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciVCAM338187:G1HKJ-3037-MONOMER
UniPathwayiUPA00359; UER00481

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:VIBHAR_03224
OrganismiVibrio campbellii (strain ATCC BAA-1116 / BB120)
Taxonomic identifieri338187 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000008152 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000494251 – 379Lipid-A-disaccharide synthaseAdd BLAST379

Interactioni

Protein-protein interaction databases

STRINGi338187.VIBHAR_03224

Structurei

3D structure databases

ProteinModelPortaliA7MY02
SMRiA7MY02
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0V Bacteria
COG0763 LUCA
HOGENOMiHOG000018003
KOiK00748
OMAiPTVWAWR

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit

Sequencei

Sequence statusi: Complete.

A7MY02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERPLRIGII AGELSGDTLG EGFIKAVKQQ YPDAEFVGIG GPKMIAQGCE
60 70 80 90 100
SLFDMEELAV MGLVEVLGRL PRLLKVKAQL VKYFTENPPD VFIGIDAPDF
110 120 130 140 150
NLRVELDLKN AGIKTVHYVS PSVWAWRQKR IFKIEAATNL VLAFLPFEKA
160 170 180 190 200
FYDKFNVPCE FIGHTLADAI PLQSDKASAR EILGLEQDKQ WLSVLPGSRG
210 220 230 240 250
SELKMLSQPF IETCKKLHQK FPDIGFVVAL VNQKRREQFE QAWKEHAPEL
260 270 280 290 300
DFKLVDDTAR NVITASDAVM LASGTVALEC MLLKRPMVVG YRVNAVTAFL
310 320 330 340 350
AKRLLKTKYV SLPNILADTE LVKEYLQDDC TPDNLFDEVS RLLESDNREM
360 370
LDKFTEMHHW IRKDADQQAA NAVLKLIEK
Length:379
Mass (Da):42,782
Last modified:October 2, 2007 - v1
Checksum:iD6ED1C97B9F9F932
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000789 Genomic DNA Translation: ABU72173.1
RefSeqiWP_012128689.1, NC_022269.1

Genome annotation databases

EnsemblBacteriaiABU72173; ABU72173; VIBHAR_03224
GeneIDi32809216
KEGGivca:M892_14955
vha:VIBHAR_03224
PATRICifig|338187.25.peg.2966

Similar proteinsi

Entry informationi

Entry nameiLPXB_VIBCB
AccessioniPrimary (citable) accession number: A7MY02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: May 23, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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