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A7MX59

- PFKA_VIBCB

UniProt

A7MX59 - PFKA_VIBCB

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Vibrio campbellii (strain ATCC BAA-1116 / BB120)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121ATP; via amide nitrogenUniRule annotation
    Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
    Active sitei128 – 1281Proton acceptorUniRule annotation
    Binding sitei155 – 1551Allosteric activator ADPUniRule annotation
    Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei212 – 2121Allosteric activator ADPUniRule annotation
    Binding sitei223 – 2231SubstrateUniRule annotation
    Binding sitei244 – 2441Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi73 – 742ATPUniRule annotation
    Nucleotide bindingi103 – 1064ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciVHAR338187:GJCH-144-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:VIBHAR_00145
    OrganismiVibrio campbellii (strain ATCC BAA-1116 / BB120)
    Taxonomic identifieri338187 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000008152: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320ATP-dependent 6-phosphofructokinasePRO_1000059808Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi338187.VIBHAR_00145.

    Structurei

    3D structure databases

    ProteinModelPortaliA7MX59.
    SMRiA7MX59. Positions 1-320.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 265Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni126 – 1283Substrate bindingUniRule annotation
    Regioni170 – 1723Substrate bindingUniRule annotation
    Regioni186 – 1883Allosteric activator ADP bindingUniRule annotation
    Regioni214 – 2163Allosteric activator ADP bindingUniRule annotation
    Regioni250 – 2534Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248870.
    KOiK00850.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7MX59-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKKIGVLTS GGDAPGMNAA VRGVVRTALS EGLEVYGVYD GYLGLYEGRI    50
    EKLDRSSVSD VINKGGTFLG SARFPEFKEV EVREQAIENL KKHGIDALVV 100
    IGGDGSYMGA KKLTEMGYPC IGLPGTIDND IAGTDYTIGY LTALNTVIDA 150
    IDRLRDTSSS HQRISIVEIM GRHCGDLTLM SAIAGGCEYI ITPETGLDKD 200
    KLIKNIQDGI AKGKKHAIIA LTELMMDANE LAREIEAATG RETRATVLGH 250
    IQRGGRPAAF DRVLASRMGN YAVHLLMEGH GGRCVGIVKE QLVHHDIIDA 300
    IENMKRPVRS DLYQVAEELF 320
    Length:320
    Mass (Da):34,630
    Last modified:October 2, 2007 - v1
    Checksum:i2A8233340C465344
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000789 Genomic DNA. Translation: ABU69193.1.
    RefSeqiYP_001443420.1. NC_009783.1.
    YP_008527085.1. NC_022269.1.

    Genome annotation databases

    EnsemblBacteriaiABU69193; ABU69193; VIBHAR_00145.
    GeneIDi16816541.
    5555128.
    KEGGivca:M892_11885.
    vha:VIBHAR_00145.
    PATRICi20127201. VBIVibHar24526_0218.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000789 Genomic DNA. Translation: ABU69193.1 .
    RefSeqi YP_001443420.1. NC_009783.1.
    YP_008527085.1. NC_022269.1.

    3D structure databases

    ProteinModelPortali A7MX59.
    SMRi A7MX59. Positions 1-320.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 338187.VIBHAR_00145.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABU69193 ; ABU69193 ; VIBHAR_00145 .
    GeneIDi 16816541.
    5555128.
    KEGGi vca:M892_11885.
    vha:VIBHAR_00145.
    PATRICi 20127201. VBIVibHar24526_0218.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248870.
    KOi K00850.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci VHAR338187:GJCH-144-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1116 / BB120.

    Entry informationi

    Entry nameiPFKA_VIBCB
    AccessioniPrimary (citable) accession number: A7MX59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3