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A7MX36

- BIOB_VIBCB

UniProt

A7MX36 - BIOB_VIBCB

Protein

Biotin synthase

Gene

bioB

Organism
Vibrio campbellii (strain ATCC BAA-1116 / BB120)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi53 – 531Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi97 – 971Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi128 – 1281Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi188 – 1881Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi260 – 2601Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciVHAR338187:GJCH-1797-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:VIBHAR_01807
    OrganismiVibrio campbellii (strain ATCC BAA-1116 / BB120)
    Taxonomic identifieri338187 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000008152: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 350350Biotin synthasePRO_0000381699Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi338187.VIBHAR_01807.

    Structurei

    3D structure databases

    ProteinModelPortaliA7MX36.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A7MX36-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVRHNWTHA EVRDLMEKPF MDLLFEAQLV HRQYQQTNYV QVSTLLSIKT    50
    GACPEDCKYC PQSARYTTDI EKERLMEVER VLDAAQKAKN AGSTRFCMGA 100
    AWKNPKERDM PHLTDMIKGV KGMGLETCMT LGMLTPDQAK QLATAGLDYY 150
    NHNLDTSPEF YGNIITTRTY QDRLDTLSHV RDAGMKICSG GIIGMGESAN 200
    DRAGLLVELA NLPVHPESVP INMLVKVKGT PLEEVDDVEP FDFIRLIAIA 250
    RIMMPQSAVR LSAGRENMNE QMQALCFMAG ANSVFYGCKL LTTPNPSEDK 300
    DMMLFNKLGI NSQEVSQKPD EIEENELLDR VVERVTARPT KDDLFYDASV 350
    Length:350
    Mass (Da):39,385
    Last modified:July 28, 2009 - v2
    Checksum:iE32E090DD16069C7
    GO

    Sequence cautioni

    The sequence ABU70776.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000789 Genomic DNA. Translation: ABU70776.1. Different initiation.
    RefSeqiYP_001445003.1. NC_009783.1.

    Genome annotation databases

    EnsemblBacteriaiABU70776; ABU70776; VIBHAR_01807.
    GeneIDi5552898.
    KEGGivha:VIBHAR_01807.
    PATRICi20130385. VBIVibHar24526_1734.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000789 Genomic DNA. Translation: ABU70776.1 . Different initiation.
    RefSeqi YP_001445003.1. NC_009783.1.

    3D structure databases

    ProteinModelPortali A7MX36.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 338187.VIBHAR_01807.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABU70776 ; ABU70776 ; VIBHAR_01807 .
    GeneIDi 5552898.
    KEGGi vha:VIBHAR_01807.
    PATRICi 20130385. VBIVibHar24526_1734.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci VHAR338187:GJCH-1797-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1116 / BB120.

    Entry informationi

    Entry nameiBIOB_VIBCB
    AccessioniPrimary (citable) accession number: A7MX36
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 47 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3