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A7MX36 (BIOB_VIBCB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:VIBHAR_01807
OrganismVibrio campbellii (strain ATCC BAA-1116 / BB120) [Complete proteome] [HAMAP]
Taxonomic identifier338187 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence ABU70776.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350Biotin synthase HAMAP-Rule MF_01694
PRO_0000381699

Sites

Metal binding531Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding571Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding971Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1281Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1881Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2601Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A7MX36 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: E32E090DD16069C7

FASTA35039,385
        10         20         30         40         50         60 
MEVRHNWTHA EVRDLMEKPF MDLLFEAQLV HRQYQQTNYV QVSTLLSIKT GACPEDCKYC 

        70         80         90        100        110        120 
PQSARYTTDI EKERLMEVER VLDAAQKAKN AGSTRFCMGA AWKNPKERDM PHLTDMIKGV 

       130        140        150        160        170        180 
KGMGLETCMT LGMLTPDQAK QLATAGLDYY NHNLDTSPEF YGNIITTRTY QDRLDTLSHV 

       190        200        210        220        230        240 
RDAGMKICSG GIIGMGESAN DRAGLLVELA NLPVHPESVP INMLVKVKGT PLEEVDDVEP 

       250        260        270        280        290        300 
FDFIRLIAIA RIMMPQSAVR LSAGRENMNE QMQALCFMAG ANSVFYGCKL LTTPNPSEDK 

       310        320        330        340        350 
DMMLFNKLGI NSQEVSQKPD EIEENELLDR VVERVTARPT KDDLFYDASV 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000789 Genomic DNA. Translation: ABU70776.1. Different initiation.
RefSeqYP_001445003.1. NC_009783.1.

3D structure databases

ProteinModelPortalA7MX36.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING338187.VIBHAR_01807.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU70776; ABU70776; VIBHAR_01807.
GeneID5552898.
KEGGvha:VIBHAR_01807.
PATRIC20130385. VBIVibHar24526_1734.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycVHAR338187:GJCH-1797-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_VIBCB
AccessionPrimary (citable) accession number: A7MX36
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways