Reviewed, UniProtKB/Swiss-Prot A7MVW7 (ASTE_VIBHB)

Names and origin

Protein names

Recommended name:
    Succinylglutamate desuccinylase
    EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	VIBHAR_02131

Organism

Vibrio harveyi (strain ATCC BAA-1116 / BB120) [Complete proteome] [HAMAP]

Taxonomic identifier

338187 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the aspA/astE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: HAMAP succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_1000017333
Sites
	Active site	219	1	Potential
	Metal binding	63	1	Zinc By similarity
	Metal binding	66	1	Zinc By similarity
	Metal binding	155	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MVW7-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: A365853723E8D2F8	FASTA	342	38,827
	10 20 30 40 50 60 MTKSLFRQSF LTDTLDVHID VAPAEQVLSN GVKLKLYQRG VLEVIPEEYT QETKNVIISC 70 80 90 100 110 120 GVHGDETAPM ELVDSIIKDI ESGFQKVEAR CLFIIAHPES TLAHSRFLEE NLNRLFDEKE 130 140 150 160 170 180 HEPTKELAIA DTLKLLVRDF YQDTAAETRW HLDLHCAIRA SKHYTFAVSP KTRHPVRSKA 190 200 210 220 230 240 LIDFLDSAHI EAVLLSNSPT STFSWFSAEN YGAQALTMEL GRVARIGEND LDKLTAFDLS 250 260 270 280 290 300 LRNLIAESKA EHLSKPCIKY RVSRTIVRLH EDFDFMFDDN VENFTSFVHG EVFGHDGDKP 310 320 330 340 LMAKNDNEAV VFPNRHVAIG QRAALMVCEV KTRYEDGELV YD

References

[1]

Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C., Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N., Pepin K., Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C., Irgon J., Wilson R.K. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000789 Genomic DNA. Translation: ABU71096.1.
RefSeq	YP_001445323.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5554743.
GenomeReviews	Gene locus VIBHAR_02131 in contig CP000789_GR.
KEGG	vha:VIBHAR_02131.
NMPDR	fig|338187.4.peg.1584.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A7MVW7. EKFAIYP.
Family and domain databases
HAMAP	MF_00767. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_VIBHB

Accession

Primary (citable) accession number: A7MVW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 2, 2007
Last modified:	June 16, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)