Luminescence regulatory protein LuxO - Vibrio harveyi (strain ATCC BAA-1116 / BB120)

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A7MVC2 (LUXO_VIBHB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 45. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Luminescence regulatory protein LuxO

Gene names

Name:	luxO
Ordered Locus Names:	VIBHAR_02959

Organism

Vibrio harveyi (strain ATCC BAA-1116 / BB120) [Complete proteome] [HAMAP]

Taxonomic identifier

338187 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio ›

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Acts negatively to control the expression of luminescence. At low cell density, LuxO is phosphorylated, and together with sigma-54, causes repression of the luxCDABEGH operon. This repression could be indirect, LuxO could activate a negative regulator of luminescence. At high cell density, LuxO is dephosphorylated and inactive, therefore the luxCDABEGH operon is not repressed and light is emitted. LuxO and sigma-54 have also a role in activating the production of siderophore and in regulating the rugose colony morphology phenotype. Ref.2

Sequence similarities

Contains 1 response regulatory domain.

Contains 1 sigma-54 factor interaction domain.

Sequence caution

The sequence ABU71912.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 453

453

Luminescence regulatory protein LuxO

PRO_0000310534

Regions

Domain

1 – 112

112

Response regulatory

Domain

133 – 362

230

Sigma-54 factor interaction

Nucleotide binding

161 – 168

ATP By similarity

Nucleotide binding

224 – 233

ATP By similarity

Amino acid modifications

Modified residue

4-aspartylphosphate Probable

Experimental info

Mutagenesis

D → K: Constitutive luminescence. Ref.2

Mutagenesis

D → A or N: Constitutive luminescence. Ref.2

Mutagenesis

D → E: Almost no light produced. Ref.2

Mutagenesis

F → W: Almost no light produced. Ref.2

Mutagenesis

K → A: Constitutive luminescence. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

A7MVC2 [UniParc].

Last modified November 13, 2007. Version 2.
Checksum: 0005EEA6F09339ED
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FASTA

453

50,459

        10         20         30         40         50         60 
MVEDTASVAA LYRSYLTPLG IDINIVGTGR DAIESLNHRI PDLILLDLRL PDMTGMDVLH 

        70         80         90        100        110        120 
AVKKSHPDVP IIFMTAHGSI DTAVEAMRHG SQDFLIKPCE ADRLRVTVNN AIRKATKLKN 

       130        140        150        160        170        180 
EADNPGNQNY QGFIGSSQTM QQVYRTIDSA ASSKASIFIT GESGTGKEVC AEAIHAASKR 

       190        200        210        220        230        240 
GDKPFIAINC AAIPKDLIES ELFGHVKGAF TGAANDRQGA AELADGGTLF LDELCEMDLD 

       250        260        270        280        290        300 
LQTKLLRFIQ TGTFQKVGSS KMKSVDVRFV CATNRDPWKE VQEGRFREDL YYRLYVIPLH 

       310        320        330        340        350        360 
LPPLRERGKD VIEIAYSLLG YMSHEEGKSF VRFAQDVIER FNSYEWPGNV RQLQNVLRNI 

       370        380        390        400        410        420 
VVLNNGKEIT LDMLPPPLNQ PVVRQSVAKF IEPDIMTVSD IMPLWMTEKM AIEQAIQACE 

       430        440        450 
GNIPRAAGYL DVSPSTIYRK LQAWNSKDEK QNV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	The Vibrio harveyi Genome Sequencing Project Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C., Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N., Pepin K., Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C., Irgon J., Wilson R.K. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1116 / BB120.
[2]	"A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi." Freeman J.A., Bassler B.L. Mol. Microbiol. 31:665-677(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-4; ASP-47; PHE-94 AND LYS-97.
[3]	"Regulation of quorum sensing in Vibrio harveyi by LuxO and sigma-54." Lilley B.N., Bassler B.L. Mol. Microbiol. 36:940-954(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SIGMA-54.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000789 Genomic DNA. Translation: ABU71912.1. Different initiation.
RefSeq	YP_001446139.1. NC_009783.1.
3D structure databases
ProteinModelPortal	A7MVC2.
ModBase	Search...
Protein-protein interaction databases
STRING	338187.VIBHAR_02959.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABU71912; ABU71912; VIBHAR_02959.
GeneID	5554621.
KEGG	vha:VIBHAR_02959.
PATRIC	20132574. VBIVibHar24526_2819.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2204.
HOGENOM	HOG000058489.
KO	K10912.
OMA	HEEGREF.
ProtClustDB	CLSK874182.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR011006. CheY-like_superfamily. IPR009057. Homeodomain-like. IPR002197. HTH_Fis. IPR001789. Sig_transdc_resp-reg_receiver. IPR002078. Sigma_54_int. IPR025943. Sigma_54_int_dom_ATP-bd_2. IPR025944. Sigma_54_int_dom_CS. [Graphical view]
Pfam	PF02954. HTH_8. 1 hit. PF00072. Response_reg. 1 hit. PF00158. Sigma54_activat. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit. SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS50110. RESPONSE_REGULATORY. 1 hit. PS00675. SIGMA54_INTERACT_1. False negative. PS00676. SIGMA54_INTERACT_2. 1 hit. PS00688. SIGMA54_INTERACT_3. 1 hit. PS50045. SIGMA54_INTERACT_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXO_VIBHB

Accession

Primary (citable) accession number: A7MVC2
Secondary accession number(s): P54299

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents