Reviewed, UniProtKB/Swiss-Prot A7MUN4 (DNAE2_VIBHB)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	VIBHAR_02868

Organism

Vibrio harveyi (strain ATCC BAA-1116 / BB120) [Complete proteome] [HAMAP]

Taxonomic identifier

338187 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	1024 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: InterPro error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1024	1024	Error-prone DNA polymerase		PRO_1000073692

Sequences

	Sequence		Length	Mass (Da)
	A7MUN4-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: A9755E574212949F	FASTA	1,024	116,906
	10 20 30 40 50 60 MSYAELFCQS NFSFLTGASH AEELVLQAAF YRYQAIAITD ECSVAGVVKA HATIEQHKLD 70 80 90 100 110 120 IKQIVGSMFW LNDECQVVLL CPCRKAYAEL CRIITNARRR SEKGSYQLSE WDLMSVRHCF 130 140 150 160 170 180 VLWLPTHQES DHYWGRWLQQ HHAHRLWVAI QRHLGGDDDA YTTHCELLAN EFQLPIAACG 190 200 210 220 230 240 GVLMHAVERL PLQHVLTAVK HGCSVDKLGF SRLSNAERAL RPLNKLSRIY KSKWLEESTH 250 260 270 280 290 300 IADLCEFKLS DLKYEYPTEL IPNGYTPTSY LRMLVERGKK LRFPEGIPAD IHQTIENELA 310 320 330 340 350 360 LIEELEYHYY FLTIHDIVMF AKQQGILYQG RGSAANSVVC YCLEITAVDP RQISVLFERF 370 380 390 400 410 420 ISREREEPPD IDVDFEHERR EEVIQYIYKK YGRERAALAA TVISYRFKSA VREVGKALGI 430 440 450 460 470 480 EETQLDFFIK NVNRRDRTQG WQAQIIELGL QPESLKGQQF IQLVNEITGF PRHLSQHVGG 490 500 510 520 530 540 FVISSGPLYE LVPVENASME DRTIIQWDKD DLESLKLLKV DVLALGMLNA IRKCFQLVEK 550 560 570 580 590 600 HHQRSLSIAE ITRLQDDPHV YRMIQKADTV GVFQIESRAQ MSMLPRLKPA RYYDLVVQIA 610 620 630 640 650 660 IARPGPIQGD MVHPFLKRRN GEEPISYPSE EVKSVLERTM GVPIFQEQVI KLAMVAAGFS 670 680 690 700 710 720 GGEADQLRRA MAAWKKNGDL AKFKPKLIDG MRERGYDLEF AERIFDQICG FGEYGFPESH 730 740 750 760 770 780 SASFAVLAYC SAWLKFYYPA EFYTALLNSQ PMGFYSPSQL IQDARRHGVE VLPVCINHSS 790 800 810 820 830 840 YQHHLVQRPN GRLGVQLGFR LVKGFNQESA SRLVERRPST GYRAIQEVKQ ILRNRRDIEL 850 860 870 880 890 900 LASADAFQIL SGNRYNARWA AMDSLSDLPL FNHIEEPMAN YQAQPSEYEN LIEDYASTGL 910 920 930 940 950 960 SLSRHPITLL EEAGKLPRFT RMKQLVEKEH NSLVTVIGVV TGRQSPGTAA GVTFFTLEDD 970 980 990 1000 1010 1020 TGNINVVVWR ATARAQKQAY LTSKVLMVKG ILEREGEVTH VIAGKLIDCT HNLAELKSKS RDFH

References

[1]

Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C., Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N., Pepin K., Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C., Irgon J., Wilson R.K. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000789 Genomic DNA. Translation: ABU71821.1.
RefSeq	YP_001446048.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5554099.
GenomeReviews	Gene locus VIBHAR_02868 in contig CP000789_GR.
KEGG	vha:VIBHAR_02868.
NMPDR	fig|338187.4.peg.2076.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_VIBHB

Accession

Primary (citable) accession number: A7MUN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 2, 2007
Last modified:	November 25, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)