A7MSZ6 (CYSH_VIBHB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	VIBHAR_00003

Organism

Vibrio harveyi (strain ATCC BAA-1116 / BB120) [Complete proteome] [HAMAP]

Taxonomic identifier

338187 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000008943

Sequences

	Sequence		Length	Mass (Da)
	A7MSZ6 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: B9FF2657D2ADE09E	FASTA	259	29,734
	10 20 30 40 50 60 MLDSVASKPE LAELLTLTKT EQILRLAQIN VELEQLSAQE RVKWALENLE GEFAVSSSFG 70 80 90 100 110 120 IQAAVMLHLV TQQKPDIPII LTDTGYLFAE TYQFIDQLKA QLNLNLKVYR AKESANWQEA 130 140 150 160 170 180 RYGKLWEQGI EGIEKYNKIN KVEPMRRALR EQNVGTWFSG LRREQSQSRA GLPILSIQNG 190 200 210 220 230 240 VFKFLPVIDW TNKDVHYYLE EHGLSYHPLW EQGYLSVGDT HTSRKWEPGM SEEETRFFGL 250 KRECGLHEDD GSEQDGSGI

References

[1]

The Vibrio harveyi Genome Sequencing Project Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C., Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N., Pepin K., Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C., Irgon J., Wilson R.K. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1116 / BB120.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000789 Genomic DNA. Translation: ABU69063.1.
RefSeq	YP_001443290.1. NC_009783.1.
3D structure databases
ProteinModelPortal	A7MSZ6.
SMR	A7MSZ6. Positions 7-248.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MSZ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5553502.
GenomeReviews	Gene locus VIBHAR_00003 in contig CP000789_GR.
KEGG	vha:VIBHAR_00003.
NMPDR	fig|338187.4.peg.46.
PATRIC	20126921. VBIVibHar24526_0090.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
HOGENOM	HBG758022.
OMA	TNFRPYE.
ProtClustDB	PRK02090.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase_CysH. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. CysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_VIBHB

Accession

Primary (citable) accession number: A7MSZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program