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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Vibrio campbellii (strain ATCC BAA-1116 / BB120)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei118Important for catalytic activityUniRule annotation1
Sitei140Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:VIBHAR_03120
OrganismiVibrio campbellii (strain ATCC BAA-1116 / BB120)
Taxonomic identifieri338187 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000008152 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000694901 – 704Fatty acid oxidation complex subunit alphaAdd BLAST704

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi338187.VIBHAR_03120.

Structurei

3D structure databases

ProteinModelPortaliA7MS61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190Enoyl-CoA hydrataseUniRule annotationAdd BLAST190
Regioni308 – 7043-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST397

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT. Bacteria.
COG1024. LUCA.
COG1250. LUCA.
HOGENOMiHOG000261346.
KOiK01782.
OMAiMMMLNEA.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 2 hits.
HAMAPiMF_01617. FadJ. 1 hit.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF51735. SSF51735. 1 hit.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MS61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQKAFSLK IDEQNIAWLA IDVPNEKMNT LQAAFADEMK EIFAQLKDTS
60 70 80 90 100
GVKGMVIHSL KPDNFVAGAD VRMLEACTTA SEAEALAKQG QELFQQLSDL
110 120 130 140 150
PYPVVAAIHG PCLGGGLELA LACDYRVCTD SDKTRLGLPE VQLGLLPGSG
160 170 180 190 200
GTQRLPRLIG LLPSLDLILT GKQLRAKKAK KLGVVDACVP ETVLLDIAKM
210 220 230 240 250
HVEKGKKKGK QKQSTKEKLM SGSGLGRKFV FEQAAKKTNE KTRGNYPATV
260 270 280 290 300
AILEVIQHGL EKGFAQGQEL EAKRFGELVM SSESKALRSI FFATTEMKKE
310 320 330 340 350
NGAEAEPTAV NKVGVLGGGL MGAGISHVSV AKAKVPVRIK DVSNDGVLNA
360 370 380 390 400
LNYNYKLFEK QRKRRIISKA GLQSKMLQLS GGIDFTSFNH IDVVIEAVFE
410 420 430 440 450
DLDLKQAMVA DIEANAKPET IFATNTSSLP IHKIAEKAER PENIVGLHYF
460 470 480 490 500
SPVEKMPLVE VIPHETTSEE TISTVVALAK KQGKTPIVVK DKAGFYVNRI
510 520 530 540 550
LAPYMNEAAH ILLANEPIEQ LDGALLDFGF PVGPITLLDE VGVDIGAKIM
560 570 580 590 600
PILVNELGER FKGPDVFDTL LNDGRKGRKT GKGFYTYKGK KKEVDKSVYK
610 620 630 640 650
LLNLTPESKL SDNDIALRCV LPMLNEAVRC LDDGIIRSPR DGDIGAIFGI
660 670 680 690 700
GFPPFLGGPF CYMDQFGLKE LVEKMNEFAS KYGDRYAPCD GLLTRAGEGR

NFYD
Length:704
Mass (Da):76,776
Last modified:October 2, 2007 - v1
Checksum:iC9C2C71B9D3797A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000789 Genomic DNA. Translation: ABU72069.1.
RefSeqiWP_012128616.1. NC_009783.1.

Genome annotation databases

EnsemblBacteriaiABU72069; ABU72069; VIBHAR_03120.
GeneIDi5553806.
KEGGivha:VIBHAR_03120.
PATRICi20132879. VBIVibHar24526_2969.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000789 Genomic DNA. Translation: ABU72069.1.
RefSeqiWP_012128616.1. NC_009783.1.

3D structure databases

ProteinModelPortaliA7MS61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi338187.VIBHAR_03120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU72069; ABU72069; VIBHAR_03120.
GeneIDi5553806.
KEGGivha:VIBHAR_03120.
PATRICi20132879. VBIVibHar24526_2969.

Phylogenomic databases

eggNOGiENOG4105DYT. Bacteria.
COG1024. LUCA.
COG1250. LUCA.
HOGENOMiHOG000261346.
KOiK01782.
OMAiMMMLNEA.

Enzyme and pathway databases

UniPathwayiUPA00659.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 2 hits.
HAMAPiMF_01617. FadJ. 1 hit.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF51735. SSF51735. 1 hit.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFADJ_VIBCB
AccessioniPrimary (citable) accession number: A7MS61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 2, 2007
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.