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A7MQS0 (SYL_CROS8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:ESA_02686
OrganismCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]
Taxonomic identifier290339 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter

Protein attributes

Sequence length860 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 860860Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009338

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7MQS0 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 6A5D75CE888663DB

FASTA86096,846
        10         20         30         40         50         60 
MQEQYRPEEI ESKVQLHWEE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VIARYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYDNINYMKN QLKMLGFGYD 

       130        140        150        160        170        180 
WSRELATCTP EYYRWEQQFF TELYKKGLVY KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD 

       190        200        210        220        230        240 
TKVERKEIPQ WFIKITAYAD ELLNDLDKLD HWPDTVKTMQ RNWIGRSEGV EITFDVQNSD 

       250        260        270        280        290        300 
EKLTVYTTRP DTFMGVTYLA VAAGHPLAQA AAAANPALGD FIAECRNTKV AEADMATMEK 

       310        320        330        340        350        360 
KGVDTGLKAI HPLTGEAIPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAT KYSLPIKPVI 

       370        380        390        400        410        420 
LTAEGTAPDL SEQALTEKGV LFNSGEFDGL DFEAAFNAIA DKLAAKGVGE RKVNYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMVTLEDG TVMPTPADQL PVILPEDVVM DGITSPIKAD PEWAKTTVNG 

       490        500        510        520        530        540 
QPALRETDTF DTFMESSWYY ARYTCPQYKE GMLDEKAANY WLPVDIYIGG IEHAIMHLLY 

       550        560        570        580        590        600 
FRFFHKLMRD AGMVNSDEPA KQLLCQGMVL ADAFYYVGAN GERNWVSPKD AIVERDEKGR 

       610        620        630        640        650        660 
IVKASDAAGH ELVYTGMSKM SKSKNNGIDP QEMVERYGAD TVRLFMMFAS PADMTLEWQE 

       670        680        690        700        710        720 
SGVEGANRFL KRVWRLVYEH TSLGDAPALN VDALNDDQKA LRRDVHKTIA KVSDDIGRRQ 

       730        740        750        760        770        780 
TFNTAIAAIM ELMNKLAKAP QEGEQDRALM REALLAVVRM LNPFTPHVSF TLWQELKGEG 

       790        800        810        820        830        840 
DIDNAPWPQA DDSAMVEDTT LVVVQVNGKV RGKITVAADA TEEQVRERAA QEHLVAKYLA 

       850        860 
GVTVRKVIYV PGKLLNLVVG 

« Hide

References

[1]"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-894.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000783 Genomic DNA. Translation: ABU77918.1.
RefSeqYP_001438754.1. NC_009778.1.

3D structure databases

ProteinModelPortalA7MQS0.
SMRA7MQS0. Positions 228-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290339.ESA_02686.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU77918; ABU77918; ESA_02686.
GeneID5550466.
KEGGesa:ESA_02686.
PATRIC20397227. VBICroSak107175_2386.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCSAK290339:GJ80-2676-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CROS8
AccessionPrimary (citable) accession number: A7MQS0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries