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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotation

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation
A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei119Important for catalytic activityUniRule annotation1
Sitei139Important for catalytic activityUniRule annotation1
Binding sitei296SubstrateUniRule annotation1
Binding sitei324NAD; via amide nitrogenUniRule annotation1
Binding sitei343NADUniRule annotation1
Binding sitei407NADUniRule annotation1
Binding sitei429NADUniRule annotation1
Active sitei450For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation1
Binding sitei453NADUniRule annotation1
Binding sitei500SubstrateUniRule annotation1
Binding sitei660SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi400 – 402NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

BioCyciCSAK290339:G1G9O-3547-MONOMER
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation, EC:5.3.3.8UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadBUniRule annotation
Ordered Locus Names:ESA_03714
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeCronobacter
Proteomesi
  • UP000000260 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000695631 – 729Fatty acid oxidation complex subunit alphaAdd BLAST729

Proteomic databases

PRIDEiA7MQP0

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotation

Protein-protein interaction databases

STRINGi290339.ESA_03714

Structurei

3D structure databases

ProteinModelPortaliA7MQP0
SMRiA7MQP0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 189Enoyl-CoA hydratase/isomeraseUniRule annotationAdd BLAST189
Regioni311 – 7293-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST419

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261344
KOiK01825
OMAiIDLCMIL

Family and domain databases

HAMAPiMF_01621 FadB, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012799 FadB
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02437 FadB, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit

Sequencei

Sequence statusi: Complete.

A7MQP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYKGDTLYL NWLEDGIAEL VFDAPGSVNK LDTATVASLG HALDVLEKQH
60 70 80 90 100
DLKALLLRSE KAAFIVGADI TEFLSLFQVP AEQLSQWLHF ANSVFNRLED
110 120 130 140 150
LPVPTLCAIN GYALGGGCEC VLATDFRLAT PDARIGLPET KLGIMPGFGG
160 170 180 190 200
SVRLPRLLGA DSALEIIAAG KDITADAALK VGLVDAVVKP EKLIEGALRM
210 220 230 240 250
LRQAIDGELD WQARRQPKLE PLRLSKIEAT MSFTIAKGMV MQTAGKHYPA
260 270 280 290 300
PMTAVKTIEA AAGLGRDEAL ALENKSFVPL ARSSEARALV GIFLNDQYVK
310 320 330 340 350
GLAKKLTKET ETPKQAAVLG AGIMGGGIAY QSAWKGVPVI MKDINEKSLT
360 370 380 390 400
LGISEASKLL NKQLERGKID GLKLAGVIAT IHPTLDYAGF ERADVVVEAV
410 420 430 440 450
VENPKVKKAV LAETEEKVRP DTVIASNTST IPISELASVL KRPENFCGMH
460 470 480 490 500
FFNPVHRMPL VEVIRGEKTS DATIAKVVSW ASKMGKTPIV VNDCPGFFVN
510 520 530 540 550
RVLFPYFAGF SQLLRDGADF RQIDKVMEKQ FGWPMGPAYL LDVVGIDTAH
560 570 580 590 600
HAQAVMAAGF PQRMQKDYRD AIDALFDAGR FGQKNGKGFY AYQEDSKGKP
610 620 630 640 650
RKVPDDAVDS LLAEVSQPKR AFSDEEIVAR MMIPMVNEVV RCLEEGIIAS
660 670 680 690 700
PAEADMALVY GLGFPPFHGG AFRWLDTQGS AKYLDMAQHY QHLGPLYEAP
710 720
AGLRDKASHN APYYPQVEPA QPVGELQTA
Length:729
Mass (Da):79,184
Last modified:October 2, 2007 - v1
Checksum:i94EAC780BCF49DC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA Translation: ABU78911.1
RefSeqiWP_012126024.1, NC_009778.1

Genome annotation databases

EnsemblBacteriaiABU78911; ABU78911; ESA_03714
KEGGiesa:ESA_03714
PATRICifig|290339.8.peg.3301

Similar proteinsi

Entry informationi

Entry nameiFADB_CROS8
AccessioniPrimary (citable) accession number: A7MQP0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 2, 2007
Last modified: April 25, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health