Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase

Gene

gppA

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities.UniRule annotation

Catalytic activityi

Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate.UniRule annotation

Pathway: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GTP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA)
  2. no protein annotated in this organism
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GTP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-3767-MONOMER.
UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphataseUniRule annotation (EC:3.6.1.40UniRule annotation)
Alternative name(s):
Guanosine pentaphosphate phosphohydrolaseUniRule annotation
pppGpp-5'-phosphohydrolaseUniRule annotation
Gene namesi
Name:gppAUniRule annotation
Ordered Locus Names:ESA_03778
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatasePRO_0000314493Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290339.ESA_03778.

Structurei

3D structure databases

ProteinModelPortaliA7MQI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GppA/Ppx family. GppA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
KOiK01524.
OMAiFKRAPQH.
OrthoDBiEOG6M6JN6.

Family and domain databases

HAMAPiMF_01550. GppA.
InterProiIPR023709. Guo-5TP_3DP_PyrP.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.

Sequencei

Sequence statusi: Complete.

A7MQI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGTPSLYAA IDLGSNSFHM LVVREVAGSI QTLTRVKRKV RLAAGLGADN
60 70 80 90 100
ALSADAMERG WQCLRLFAER LQDIPPSQIR VVATATLRLA TNADVFVARA
110 120 130 140 150
QEILGCPVQV IAGEEEARLI YQGVAHTTGG ADRRLVVDIG GASTELVTGT
160 170 180 190 200
GARATSLFSL PMGCVTFLER FFTDRSLTQE HFASAEQAAR DILQPVIAQL
210 220 230 240 250
RGQGWKICVG ASGTVQALQE IMMAQGMDER ITLAKLQQLK ARAIHCGRLE
260 270 280 290 300
ELEIEGLTLE RALVFPSGLA ILIAIFSELE IECMTLAGGA LREGLVYGML
310 320 330 340 350
HLAVDQDIRN RTLKNIQRRF MVDIAQAGRV AQLAERFAVS VEREWALDDL
360 370 380 390 400
SRALLHSASL LHEVGLAVDF KRAPQHAAYL VNNLDLPGFT PAQKKLLATL
410 420 430 440 450
LLNQTGTVDL PALQQQNAVP LRVAERLCRL LRLAIIFASR RRDDMLPDVT
460 470 480 490
LTAHDDTLTA SLPQDWLSVH PLGAEMLEQE QLWQSYVHWQ LDVK
Length:494
Mass (Da):54,237
Last modified:October 2, 2007 - v1
Checksum:i93EB6216DE0729E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU78970.1.
RefSeqiWP_012126057.1. NC_009778.1.
YP_001439806.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU78970; ABU78970; ESA_03778.
GeneIDi5548290.
KEGGiesa:ESA_03778.
PATRICi20399237. VBICroSak107175_3359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU78970.1.
RefSeqiWP_012126057.1. NC_009778.1.
YP_001439806.1. NC_009778.1.

3D structure databases

ProteinModelPortaliA7MQI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290339.ESA_03778.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU78970; ABU78970; ESA_03778.
GeneIDi5548290.
KEGGiesa:ESA_03778.
PATRICi20399237. VBICroSak107175_3359.

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
KOiK01524.
OMAiFKRAPQH.
OrthoDBiEOG6M6JN6.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.
BioCyciCSAK290339:GJ80-3767-MONOMER.

Family and domain databases

HAMAPiMF_01550. GppA.
InterProiIPR023709. Guo-5TP_3DP_PyrP.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiGPPA_CROS8
AccessioniPrimary (citable) accession number: A7MQI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: May 27, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.