A7MPJ7 (ACSA_CROS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acs
Ordered Locus Names:	ESA_00122

Organism

Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	652 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Enables the cell to use acetate during aerobic growth to generate energy via the TCA cycle, and biosynthetic compounds via the glyoxylate shunt. Acetylates CheY, the response regulator involved in flagellar movement and chemotaxis By similarity. HAMAP MF_01123
Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 652	652	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000065290
Sites
	Active site	517	1	By similarity
Amino acid modifications
	Modified residue	609	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MPJ7 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: E13500326E51D759	FASTA	652	71,752
	10 20 30 40 50 60 MSQIHKHPIP AAIAERCLIT PEQYDAKYQQ SVHDPDAFWG EQGKILDWIT PYQTVKNTSF 70 80 90 100 110 120 APGNVSIKWY EDGTLNLAAN CLDRHLSERG DQTAIIWEGD DATQSKHISY RELHRDVCRF 130 140 150 160 170 180 ANTLTSLGIK KGDVVAIYMP MVPEAAVAML ACARIGAVHS VIFGGFSPEA VAGRIIDSSA 190 200 210 220 230 240 RLVITADEGV RAGRAIPLKK NVDDALKNPG VTSVEHVVVF KRTGGNIEWH EGRDLWWSEL 250 260 270 280 290 300 VKKASAHHQP VEMNAEDPLF ILYTSGSTGK PKGVLHTTGG YLVYAATTFL YAFDYHPGDI 310 320 330 340 350 360 YWCTADVGWV TGHSYLLYGP LACGATTLMF EGVPNWPSAN RMAQVVDKHK VNILYTAPTA 370 380 390 400 410 420 IRALMAEGDK AIDGTDRSSL RILGSVGEPI NPEAWEWYWK KIGNEKCPVI DTWWQTETGG 430 440 450 460 470 480 FMITPLPGAT ELKAGSATRP FFGVQPALVD NEGNPQQGAT EGNLVITDSW PGQARTLFGD 490 500 510 520 530 540 HERFEQTYFS TFKNMYFSGD GARRDEDGYY WITGRVDDVL NVSGHRLGTA EIESALVSHP 550 560 570 580 590 600 KIAEAAVVGI PHSIKGQAIY AYVTLNHGEE PSAELYSEVR NWVRKEIGPL ATPDVLHWTD 610 620 630 640 650 SLPKTRSGKI MRRILRKIAA GDTSNLGDTS TLADPGVVEK LLEEKQSIAM PS

References

[1]

"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species." Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J. PLoS ONE 5:E9556-E9556(2010) [PubMed: 20221447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-894.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU75427.1.
RefSeq	YP_001436263.1. NC_009778.1.
3D structure databases
ProteinModelPortal	A7MPJ7.
SMR	A7MPJ7. Positions 5-647.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MPJ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5549082.
GenomeReviews	Gene locus ESA_00122 in contig CP000783_GR.
KEGG	esa:ESA_00122.
PATRIC	20392563. VBICroSak107175_0109.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0365.
HOGENOM	HBG547964.
OMA	LIQWAGG.
ProtClustDB	PRK00174.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_CROS8

Accession

Primary (citable) accession number: A7MPJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program