Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methionyl-tRNA formyltransferase

Gene

fmt

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP.UniRule annotation

Catalytic activityi

10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet).UniRule annotation

GO - Molecular functioni

  1. methionyl-tRNA formyltransferase activity Source: UniProtKB-HAMAP
  2. methyltransferase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-35-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionyl-tRNA formyltransferaseUniRule annotation (EC:2.1.2.9UniRule annotation)
Gene namesi
Name:fmtUniRule annotation
Ordered Locus Names:ESA_00038
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Methionyl-tRNA formyltransferasePRO_1000020060Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290339.ESA_00038.

Structurei

3D structure databases

ProteinModelPortaliA7MPE8.
SMRiA7MPE8. Positions 2-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni113 – 1164Tetrahydrofolate (THF) bindingUniRule annotation

Sequence similaritiesi

Belongs to the Fmt family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0223.
HOGENOMiHOG000261177.
KOiK00604.
OMAiKVWQSRV.
OrthoDBiEOG6B09WV.

Family and domain databases

Gene3Di3.10.25.10. 1 hit.
3.40.50.170. 1 hit.
HAMAPiMF_00182. Formyl_trans.
InterProiIPR005794. Fmt.
IPR005793. Formyl_trans_C.
IPR002376. Formyl_transf_N.
IPR011034. Formyl_transferase_C-like.
IPR001555. GART_AS.
IPR015518. Met_tRNA_Form_TA-like.
[Graphical view]
PANTHERiPTHR11138. PTHR11138. 1 hit.
PfamiPF02911. Formyl_trans_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
SSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00460. fmt. 1 hit.
PROSITEiPS00373. GART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MPE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSESLRIIFA GTPDFAARHL DALLSSSHQV VGVFTQPDRP AGRGKKLMPG
60 70 80 90 100
PVKVLAQEND IPVFQPKSLR SAENQELVAA LNADVMVVVA YGLILPEAVL
110 120 130 140 150
SMPRLGCINV HGSLLPRWRG AAPIQRSLWA GDAETGVTIM QMDKGLDTGD
160 170 180 190 200
MLRKLSCPIT ADDTSASLYD KLAQLGPQGL LATLEDLAAG RAVPEKQDDA
210 220 230 240 250
QATYADKLSK EEARLDWTLS AAQLERCIRA FNPWPVSFFM IDEQPVKVWK
260 270 280 290 300
ASVIRQQSHA TPGTILDAGK QGIQVATTDG ILNLESLQPA GKKPMSAQDL
310
LNSRREWFTP GAILA
Length:315
Mass (Da):34,084
Last modified:October 1, 2007 - v1
Checksum:i42C263EA3F2AB1CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU75347.1.
RefSeqiWP_012123588.1. NC_009778.1.
YP_001436183.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU75347; ABU75347; ESA_00038.
GeneIDi5548819.
KEGGiesa:ESA_00038.
PATRICi20392407. VBICroSak107175_0036.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU75347.1.
RefSeqiWP_012123588.1. NC_009778.1.
YP_001436183.1. NC_009778.1.

3D structure databases

ProteinModelPortaliA7MPE8.
SMRiA7MPE8. Positions 2-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290339.ESA_00038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU75347; ABU75347; ESA_00038.
GeneIDi5548819.
KEGGiesa:ESA_00038.
PATRICi20392407. VBICroSak107175_0036.

Phylogenomic databases

eggNOGiCOG0223.
HOGENOMiHOG000261177.
KOiK00604.
OMAiKVWQSRV.
OrthoDBiEOG6B09WV.

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-35-MONOMER.

Family and domain databases

Gene3Di3.10.25.10. 1 hit.
3.40.50.170. 1 hit.
HAMAPiMF_00182. Formyl_trans.
InterProiIPR005794. Fmt.
IPR005793. Formyl_trans_C.
IPR002376. Formyl_transf_N.
IPR011034. Formyl_transferase_C-like.
IPR001555. GART_AS.
IPR015518. Met_tRNA_Form_TA-like.
[Graphical view]
PANTHERiPTHR11138. PTHR11138. 1 hit.
PfamiPF02911. Formyl_trans_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
SSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00460. fmt. 1 hit.
PROSITEiPS00373. GART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiFMT_CROS8
AccessioniPrimary (citable) accession number: A7MPE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 14, 2008
Last sequence update: October 1, 2007
Last modified: March 31, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.