A7MPD7 (ACEK_CROS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	ESA_00055

Organism

Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000315264
Regions
	Nucleotide binding	316 – 322	7	ATP By similarity
Sites
	Active site	372	1	By similarity
	Binding site	337	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MPD7 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: B28E23C061AEC6B4	FASTA	596	69,185
	10 20 30 40 50 60 MPPRGLELLI AQTILQGFDA QYGRFLEITA GAQHRFEQAD WHAVQQAMKS RIHLYDHHVG 70 80 90 100 110 120 LVVEQLRCIM EGKATDPDFL LRVKRHYTDL LPDYPRFEIA ESFFNSVYCR LYDHRSLTPD 130 140 150 160 170 180 RLFIFSSQPE RRFRTMPRPL AKNFFPEQGW QPLLTTLLTD LPLRLPWQDL ARDVRYIIAH 190 200 210 220 230 240 LNETLGPARL AQAHLEMANE LFYRNKAAWL VGKLRLPDAT LPFLLPVHRS DDGELFVDTC 250 260 270 280 290 300 LTGSAGASIV FGFARSYFMV YAPLPAALVE WLREILPGKT TAELYMAIGC QKHAKTESYR 310 320 330 340 350 360 EYLHYLRHAD EQFIEAPGIR GMVMLVFTLP GFDRVFKVIK DRFAPQKEMS EAHVRACYQL 370 380 390 400 410 420 VKEHDRVGRM ADTQEFENFV IEKRRISPEL MALLQQEVGQ KLTDMGDKIS ISHLYIERRM 430 440 450 460 470 480 VPLNIWLEQA TGQQLRDAIE EYGNAIRQLA AANIFPGDML FKNFGVTRHG RVVFYDYDEI 490 500 510 520 530 540 CYMTEVNFRD IPPPRYPEDE LSAEPWYSVA PGDVFPEEFR HWLCADPKIG PLFEEMHSDL 550 560 570 580 590 FSASYWRALQ TRIREGYVED VYAYRRRQRF CVRWKNHNGH PEAAVSAIAP SAVGEG

References

[1]

"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species." Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J. PLoS ONE 5:E9556-E9556(2010) [PubMed: 20221447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-894.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU75360.1.
RefSeq	YP_001436196.1. NC_009778.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7MPD7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5548932.
GenomeReviews	Gene locus ESA_00055 in contig CP000783_GR.
KEGG	esa:ESA_00055.
PATRIC	20392441. VBICroSak107175_0048.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
ProtClustDB	PRK02946.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_CROS8

Accession

Primary (citable) accession number: A7MPD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program