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A7MPB4 (NUOH_CROS8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit H

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit H
NDH-1 subunit H
Gene names
Name:nuoH
Ordered Locus Names:ESA_00938
OrganismCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]
Taxonomic identifier290339 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone By similarity. HAMAP-Rule MF_01350

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01350

Subunit structure

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.

Subcellular location

Cell inner membrane; Multi-pass membrane protein Potential HAMAP-Rule MF_01350.

Sequence similarities

Belongs to the complex I subunit 1 family.

Ontologies

Keywords
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandNAD
Ubiquinone
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADH dehydrogenase (quinone) activity

Inferred from electronic annotation. Source: UniProtKB-EC

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326NADH-quinone oxidoreductase subunit H HAMAP-Rule MF_01350
PRO_1000067744

Regions

Transmembrane11 – 3121Helical; Potential
Transmembrane81 – 10121Helical; Potential
Transmembrane114 – 13421Helical; Potential
Transmembrane154 – 17421Helical; Potential
Transmembrane186 – 20621Helical; Potential
Transmembrane237 – 25721Helical; Potential
Transmembrane265 – 28521Helical; Potential
Transmembrane302 – 32221Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
A7MPB4 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 4E8AF4D21687BFE2

FASTA32636,331
        10         20         30         40         50         60 
MSWLTPDVIE ILLSILKAVV ILLVVVSCGA FMSFGERRLL GLFQNRYGPN RVGWGGSLQL 

        70         80         90        100        110        120 
VADMIKMFFK EDWIPRFSDR VIFTLAPMIA FTSLLLAFAI VPVSPTWVVA DLNIGILFFL 

       130        140        150        160        170        180 
MMAGLGVYAV LFAGWSSNNK YSLLGAMRAS AQTLSYEVFL GLSLMGVVAQ AGSFNMTDIV 

       190        200        210        220        230        240 
NNQAHLWNVI PQFFGFITFA IAGVAVCHRH PFDQPEAEQE LADGYHIEYS GMKFGLFFVG 

       250        260        270        280        290        300 
EYIGIVTVSA LIVTLFFGGW QGPWLPPFIW FALKTAFFMM MFILIRAALP RPRYDQVMSF 

       310        320 
GWKVCLPLTL INLLVTAAVI LWQTAA 

« Hide

References

[1]"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-894.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000783 Genomic DNA. Translation: ABU76208.1.
RefSeqYP_001437044.1. NC_009778.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290339.ESA_00938.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU76208; ABU76208; ESA_00938.
GeneID5552247.
KEGGesa:ESA_00938.
PATRIC20394071. VBICroSak107175_0836.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1005.
HOGENOMHOG000228276.
KOK00337.
OMALDLGWKF.
OrthoDBEOG6B62B4.
ProtClustDBPRK06076.

Enzyme and pathway databases

BioCycCSAK290339:GJ80-932-MONOMER.

Family and domain databases

HAMAPMF_01350. NDH1_NuoH.
InterProIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERPTHR11432. PTHR11432. 1 hit.
PfamPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNUOH_CROS8
AccessionPrimary (citable) accession number: A7MPB4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 2, 2007
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families