Reviewed, UniProtKB/Swiss-Prot A7MNW9 (NADE_ENTS8)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	ESA_02148

Organism

Enterobacter sakazakii (strain ATCC BAA-894) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077556
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MNW9-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: DE321C6FA967930C	FASTA	275	30,826
	10 20 30 40 50 60 MALQQEIIQA LGVKPQIDAH EEIRRSVDFL KSYLKTYPFL KTLVLGISGG QDSTLAGKLS 70 80 90 100 110 120 QLAISELRDE TGDQSYQFIA VRLPFGVQFD EKDCQDALAF IQPDKVLTVN IKEAVLASEK 130 140 150 160 170 180 ALREAGIELS DFVRGNEKAR ERMKAQYSIA GMTKGVVVGT DHAAEAVTGF FTKYGDGGTD 190 200 210 220 230 240 INPLFRLNKR QGKLLLKTLG CPEHLYLKVP TADLEDDRPS LPDEVALGVT YDNIDDYLEG 250 260 270 KQIDEKISQI IDGWYVKTEH KRRPPITIFD DFWKQ

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton B., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000783 Genomic DNA. Translation: ABU77397.1.
RefSeq	YP_001438233.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7MNW9.
Genome annotation databases
GeneID	5550101.
GenomeReviews	Gene locus ESA_02148 in contig CP000783_GR.
KEGG	esa:ESA_02148.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ADLEEDR.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_ENTS8

Accession

Primary (citable) accession number: A7MNW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 2, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)