A7MNV8 (ASTD_CROS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-succinylglutamate 5-semialdehyde dehydrogenase
EC=1.2.1.71

Alternative name(s):
Succinylglutamic semialdehyde dehydrogenase
Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	ESA_02155

Organism

Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity. HAMAP MF_01174
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_1000065757
Regions
	Nucleotide binding	220 – 225	6	NAD By similarity
Sites
	Active site	243	1	By similarity
	Active site	277	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MNV8 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 71E87D01B57FE4BC	FASTA	492	52,433
	10 20 30 40 50 60 MSLWINGEWT TGQGEALEKR DPVGSGLLWQ GHAADDAQVQ AACAAARAAF PAWAKRPFAD 70 80 90 100 110 120 RQAIAEKFAA LLEANKTELT RIIALETSKP RWEAATEVTA MINKVGISLK AYQTRTGEQH 130 140 150 160 170 180 SPMPDGAATL RHRPHGVLAV FGPYNFPGHL PNGHIVPALL AGNTLVFKPS ELTPHTGEAV 190 200 210 220 230 240 MKLWEQAGLP AGVLNLVQGA RATGQALSAR PELDGLLFTG SAGTGYQLHR QLAGQPEKIL 250 260 270 280 290 300 ALEMGGNNPL IVEDPDDIDG AVHLAIQSAF ITAGQRCTCA RRLLVKRGAA GDAFLARLVE 310 320 330 340 350 360 IAGRLRPDRW DAEPQPFMGG LISAQAAERV LEAWQGHLSR GGKPLLTPAL VQAGSSLLTP 370 380 390 400 410 420 GIIELTGVRD VPDEEVFGPL LGVWRYDDFD EAITLANATR YGLSCGLISP VREKFDQLLL 430 440 450 460 470 480 EARAGIVNWN KPLTGAASTA PFGGVGASGN HRPSAWYAAD YCAWPMASLE SPTFALPQTL 490 NPGLDFSGKE QA

References

[1]

"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species." Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J. PLoS ONE 5:E9556-E9556(2010) [PubMed: 20221447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-894.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU77404.1.
RefSeq	YP_001438240.1. NC_009778.1.
3D structure databases
ProteinModelPortal	A7MNV8.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MNV8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5550034.
GenomeReviews	Gene locus ESA_02155 in contig CP000783_GR.
KEGG	esa:ESA_02155.
PATRIC	20396277. VBICroSak107175_1926.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HBG752218.
OMA	KAYHART.
ProtClustDB	CLSK2539907.
Family and domain databases
HAMAP	MF_01174. Aldedh_AstD. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
KO	K06447.
PANTHER	PTHR11699:SF24. PTHR11699:SF24. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR03240. Arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_CROS8

Accession

Primary (citable) accession number: A7MNV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program