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Protein

Beta-galactosidase

Gene

lacZ

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei113 – 1131SubstrateUniRule annotation
Metal bindingi212 – 2121SodiumUniRule annotation
Binding sitei212 – 2121SubstrateUniRule annotation
Sitei367 – 3671Transition state stabilizerUniRule annotation
Sitei401 – 4011Transition state stabilizerUniRule annotation
Metal bindingi426 – 4261Magnesium 1UniRule annotation
Metal bindingi428 – 4281Magnesium 1UniRule annotation
Active sitei471 – 4711Proton donorUniRule annotation
Metal bindingi471 – 4711Magnesium 1UniRule annotation
Binding sitei471 – 4711SubstrateUniRule annotation
Active sitei547 – 5471NucleophileUniRule annotation
Metal bindingi607 – 6071Magnesium 2UniRule annotation
Metal bindingi611 – 6111Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi614 – 6141SodiumUniRule annotation
Binding sitei614 – 6141SubstrateUniRule annotation
Binding sitei1017 – 10171SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-2967-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:ESA_02977
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10431043Beta-galactosidasePRO_0000366984Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi290339.ESA_02977.

Structurei

3D structure databases

ProteinModelPortaliA7MN76.
SMRiA7MN76. Positions 24-1036.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni547 – 5504Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiCEYAHDM.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MN76-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTENPTTTAF NELQTRPLAT ILARNDWQNP AITSVNRLPS HTPLHGWRDA
60 70 80 90 100
DRARRGEPSD AVLSLDGEWQ FSFFASPHQV PEVWLAADLS DARATPVPSN
110 120 130 140 150
WQMEGYDTPI YTNVRYPIPV NPPFVPDDNP TGCYSRDIEV PQAWLETGRT
160 170 180 190 200
RIIFGGVNSA FYLWCNGQWV GYSQDSRLPA EFDLTGVLHA GRNRLCVLVL
210 220 230 240 250
RWSDGTYLED QDMWRMSGIF RPVSLLHLPE QALADVRVHT ELAPSLRHAT
260 270 280 290 300
LFSEVEVTPA ACGLSVTLAL WHGENEIASQ TLPLGSAPID ERGNYAERVT
310 320 330 340 350
LSLDVDNPLL WSAETPHLYR AVVTLLDADG MPLVSEAHDV GFRRVEINNG
360 370 380 390 400
LLTLNGQPLL IRGVNRHEHH PEKGQAVDEA AMVQDILLMK QNNFNAVRCS
410 420 430 440 450
HYPNQPRWYE LCSRYGLYVV DEANIETHGM EPMSRLSDDP VWLGAYSERV
460 470 480 490 500
TRMVKCNRNH PSIIIWSLGN ESGNGATHTA LYNWIKHQDP TRPVQYEGGG
510 520 530 540 550
ADTRATDIIC PMYARVETDQ LIPAVPKWSI KKWISMPGET RPLILCEYAH
560 570 580 590 600
AMGNSLGNFA DYWAAFRQYP RLQGGFIWDW ADQAITRVEP DGSRWWAYGG
610 620 630 640 650
DFGDTPNDRQ FCMNGLVFPD RTPHPALFEA KHQQQFFQFR LVSENPLQIE
660 670 680 690 700
VTSEYLFRES DNERLLWSIE VRGETRLSGE LTLELGPQAS RVLTLSDTGF
710 720 730 740 750
SARAGDEEIW LHVRVEQPQA TPWSPEGHLS AWAQWPLAAP LALPEPVAAG
760 770 780 790 800
DAPQLEITDA EFVIRHGCQT WRVSRASGQL IQWSDDGVDQ ILTPLADQFI
810 820 830 840 850
RAPIDNDIGV SEVERIDPNA WVERWKAAGL YNTEHRCLAC DAQTTRDGVE
860 870 880 890 900
IVAQHAYFVK GVADGPAILS RWRMVVDNQG ALHCDIDIAR SAALPPLPRV
910 920 930 940 950
GVVCQLRGGE ETASWLGLGP HENYPDRLSS ACFSRWTLPL SELTTPYIFP
960 970 980 990 1000
GENGLRCNTR ELNWNGWQAE GEFHFSLSPY GTRQLMETSH WHKLQPEAGI
1010 1020 1030 1040
WLTIDGFHMG VGGDDSWTPS VHPEYLLTAR EYRYRFTLRR RQG
Length:1,043
Mass (Da):117,662
Last modified:October 2, 2007 - v1
Checksum:i269BB0FA74F2772F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU78206.1.
RefSeqiWP_012125561.1. NC_009778.1.
YP_001439042.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU78206; ABU78206; ESA_02977.
GeneIDi5548613.
KEGGiesa:ESA_02977.
PATRICi20397789. VBICroSak107175_2666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU78206.1.
RefSeqiWP_012125561.1. NC_009778.1.
YP_001439042.1. NC_009778.1.

3D structure databases

ProteinModelPortaliA7MN76.
SMRiA7MN76. Positions 24-1036.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290339.ESA_02977.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU78206; ABU78206; ESA_02977.
GeneIDi5548613.
KEGGiesa:ESA_02977.
PATRICi20397789. VBICroSak107175_2666.

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiCEYAHDM.
OrthoDBiEOG6XWV0T.

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-2967-MONOMER.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiBGAL_CROS8
AccessioniPrimary (citable) accession number: A7MN76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 2, 2007
Last modified: February 4, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.