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A7MN76

- BGAL_CROS8

UniProt

A7MN76 - BGAL_CROS8

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Protein

Beta-galactosidase

Gene
lacZ, ESA_02977
Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei113 – 1131Substrate By similarity
Metal bindingi212 – 2121Sodium By similarity
Binding sitei212 – 2121Substrate By similarity
Sitei367 – 3671Transition state stabilizer By similarity
Sitei401 – 4011Transition state stabilizer By similarity
Metal bindingi426 – 4261Magnesium 1 By similarity
Metal bindingi428 – 4281Magnesium 1 By similarity
Active sitei471 – 4711Proton donor By similarity
Metal bindingi471 – 4711Magnesium 1 By similarity
Binding sitei471 – 4711Substrate By similarity
Active sitei547 – 5471Nucleophile By similarity
Metal bindingi607 – 6071Magnesium 2 By similarity
Metal bindingi611 – 6111Sodium; via carbonyl oxygen By similarity
Metal bindingi614 – 6141Sodium By similarity
Binding sitei614 – 6141Substrate By similarity
Binding sitei1017 – 10171Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-2967-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:ESA_02977
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10431043Beta-galactosidaseUniRule annotationPRO_0000366984Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi290339.ESA_02977.

Structurei

3D structure databases

ProteinModelPortaliA7MN76.
SMRiA7MN76. Positions 24-1036.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni547 – 5504Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiSHLINME.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MN76-1 [UniParc]FASTAAdd to Basket

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MTENPTTTAF NELQTRPLAT ILARNDWQNP AITSVNRLPS HTPLHGWRDA     50
DRARRGEPSD AVLSLDGEWQ FSFFASPHQV PEVWLAADLS DARATPVPSN 100
WQMEGYDTPI YTNVRYPIPV NPPFVPDDNP TGCYSRDIEV PQAWLETGRT 150
RIIFGGVNSA FYLWCNGQWV GYSQDSRLPA EFDLTGVLHA GRNRLCVLVL 200
RWSDGTYLED QDMWRMSGIF RPVSLLHLPE QALADVRVHT ELAPSLRHAT 250
LFSEVEVTPA ACGLSVTLAL WHGENEIASQ TLPLGSAPID ERGNYAERVT 300
LSLDVDNPLL WSAETPHLYR AVVTLLDADG MPLVSEAHDV GFRRVEINNG 350
LLTLNGQPLL IRGVNRHEHH PEKGQAVDEA AMVQDILLMK QNNFNAVRCS 400
HYPNQPRWYE LCSRYGLYVV DEANIETHGM EPMSRLSDDP VWLGAYSERV 450
TRMVKCNRNH PSIIIWSLGN ESGNGATHTA LYNWIKHQDP TRPVQYEGGG 500
ADTRATDIIC PMYARVETDQ LIPAVPKWSI KKWISMPGET RPLILCEYAH 550
AMGNSLGNFA DYWAAFRQYP RLQGGFIWDW ADQAITRVEP DGSRWWAYGG 600
DFGDTPNDRQ FCMNGLVFPD RTPHPALFEA KHQQQFFQFR LVSENPLQIE 650
VTSEYLFRES DNERLLWSIE VRGETRLSGE LTLELGPQAS RVLTLSDTGF 700
SARAGDEEIW LHVRVEQPQA TPWSPEGHLS AWAQWPLAAP LALPEPVAAG 750
DAPQLEITDA EFVIRHGCQT WRVSRASGQL IQWSDDGVDQ ILTPLADQFI 800
RAPIDNDIGV SEVERIDPNA WVERWKAAGL YNTEHRCLAC DAQTTRDGVE 850
IVAQHAYFVK GVADGPAILS RWRMVVDNQG ALHCDIDIAR SAALPPLPRV 900
GVVCQLRGGE ETASWLGLGP HENYPDRLSS ACFSRWTLPL SELTTPYIFP 950
GENGLRCNTR ELNWNGWQAE GEFHFSLSPY GTRQLMETSH WHKLQPEAGI 1000
WLTIDGFHMG VGGDDSWTPS VHPEYLLTAR EYRYRFTLRR RQG 1043
Length:1,043
Mass (Da):117,662
Last modified:October 2, 2007 - v1
Checksum:i269BB0FA74F2772F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000783 Genomic DNA. Translation: ABU78206.1.
RefSeqiYP_001439042.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU78206; ABU78206; ESA_02977.
GeneIDi5548613.
KEGGiesa:ESA_02977.
PATRICi20397789. VBICroSak107175_2666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000783 Genomic DNA. Translation: ABU78206.1 .
RefSeqi YP_001439042.1. NC_009778.1.

3D structure databases

ProteinModelPortali A7MN76.
SMRi A7MN76. Positions 24-1036.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290339.ESA_02977.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABU78206 ; ABU78206 ; ESA_02977 .
GeneIDi 5548613.
KEGGi esa:ESA_02977.
PATRICi 20397789. VBICroSak107175_2666.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi SHLINME.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci CSAK290339:GJ80-2967-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiBGAL_CROS8
AccessioniPrimary (citable) accession number: A7MN76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 2, 2007
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi