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A7MN76 (BGAL_CROS8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:ESA_02977
OrganismCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]
Taxonomic identifier290339 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter

Protein attributes

Sequence length1043 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10431043Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000366984

Regions

Region547 – 5504Substrate binding By similarity

Sites

Active site4711Proton donor By similarity
Active site5471Nucleophile By similarity
Metal binding2121Sodium By similarity
Metal binding4261Magnesium 1 By similarity
Metal binding4281Magnesium 1 By similarity
Metal binding4711Magnesium 1 By similarity
Metal binding6071Magnesium 2 By similarity
Metal binding6111Sodium; via carbonyl oxygen By similarity
Metal binding6141Sodium By similarity
Binding site1131Substrate By similarity
Binding site2121Substrate By similarity
Binding site4711Substrate By similarity
Binding site6141Substrate By similarity
Binding site10171Substrate By similarity
Site3671Transition state stabilizer By similarity
Site4011Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A7MN76 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 269BB0FA74F2772F

FASTA1,043117,662
        10         20         30         40         50         60 
MTENPTTTAF NELQTRPLAT ILARNDWQNP AITSVNRLPS HTPLHGWRDA DRARRGEPSD 

        70         80         90        100        110        120 
AVLSLDGEWQ FSFFASPHQV PEVWLAADLS DARATPVPSN WQMEGYDTPI YTNVRYPIPV 

       130        140        150        160        170        180 
NPPFVPDDNP TGCYSRDIEV PQAWLETGRT RIIFGGVNSA FYLWCNGQWV GYSQDSRLPA 

       190        200        210        220        230        240 
EFDLTGVLHA GRNRLCVLVL RWSDGTYLED QDMWRMSGIF RPVSLLHLPE QALADVRVHT 

       250        260        270        280        290        300 
ELAPSLRHAT LFSEVEVTPA ACGLSVTLAL WHGENEIASQ TLPLGSAPID ERGNYAERVT 

       310        320        330        340        350        360 
LSLDVDNPLL WSAETPHLYR AVVTLLDADG MPLVSEAHDV GFRRVEINNG LLTLNGQPLL 

       370        380        390        400        410        420 
IRGVNRHEHH PEKGQAVDEA AMVQDILLMK QNNFNAVRCS HYPNQPRWYE LCSRYGLYVV 

       430        440        450        460        470        480 
DEANIETHGM EPMSRLSDDP VWLGAYSERV TRMVKCNRNH PSIIIWSLGN ESGNGATHTA 

       490        500        510        520        530        540 
LYNWIKHQDP TRPVQYEGGG ADTRATDIIC PMYARVETDQ LIPAVPKWSI KKWISMPGET 

       550        560        570        580        590        600 
RPLILCEYAH AMGNSLGNFA DYWAAFRQYP RLQGGFIWDW ADQAITRVEP DGSRWWAYGG 

       610        620        630        640        650        660 
DFGDTPNDRQ FCMNGLVFPD RTPHPALFEA KHQQQFFQFR LVSENPLQIE VTSEYLFRES 

       670        680        690        700        710        720 
DNERLLWSIE VRGETRLSGE LTLELGPQAS RVLTLSDTGF SARAGDEEIW LHVRVEQPQA 

       730        740        750        760        770        780 
TPWSPEGHLS AWAQWPLAAP LALPEPVAAG DAPQLEITDA EFVIRHGCQT WRVSRASGQL 

       790        800        810        820        830        840 
IQWSDDGVDQ ILTPLADQFI RAPIDNDIGV SEVERIDPNA WVERWKAAGL YNTEHRCLAC 

       850        860        870        880        890        900 
DAQTTRDGVE IVAQHAYFVK GVADGPAILS RWRMVVDNQG ALHCDIDIAR SAALPPLPRV 

       910        920        930        940        950        960 
GVVCQLRGGE ETASWLGLGP HENYPDRLSS ACFSRWTLPL SELTTPYIFP GENGLRCNTR 

       970        980        990       1000       1010       1020 
ELNWNGWQAE GEFHFSLSPY GTRQLMETSH WHKLQPEAGI WLTIDGFHMG VGGDDSWTPS 

      1030       1040 
VHPEYLLTAR EYRYRFTLRR RQG 

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References

[1]"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-894.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000783 Genomic DNA. Translation: ABU78206.1.
RefSeqYP_001439042.1. NC_009778.1.

3D structure databases

ProteinModelPortalA7MN76.
SMRA7MN76. Positions 24-1036.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290339.ESA_02977.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU78206; ABU78206; ESA_02977.
GeneID5548613.
KEGGesa:ESA_02977.
PATRIC20397789. VBICroSak107175_2666.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMAVYNENFF.
OrthoDBEOG6XWV0T.
ProtClustDBPRK09525.

Enzyme and pathway databases

BioCycCSAK290339:GJ80-2967-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_CROS8
AccessionPrimary (citable) accession number: A7MN76
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 2, 2007
Last modified: February 19, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries