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Protein

Beta-galactosidase

Gene

lacZ

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei113SubstrateUniRule annotation1
Metal bindingi212SodiumUniRule annotation1
Binding sitei212SubstrateUniRule annotation1
Sitei367Transition state stabilizerUniRule annotation1
Sitei401Transition state stabilizerUniRule annotation1
Metal bindingi426Magnesium 1UniRule annotation1
Metal bindingi428Magnesium 1UniRule annotation1
Active sitei471Proton donorUniRule annotation1
Metal bindingi471Magnesium 1UniRule annotation1
Binding sitei471SubstrateUniRule annotation1
Active sitei547NucleophileUniRule annotation1
Metal bindingi607Magnesium 2UniRule annotation1
Metal bindingi611Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi614SodiumUniRule annotation1
Binding sitei614SubstrateUniRule annotation1
Binding sitei1017SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMagnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciCSAK290339:G1G9O-2835-MONOMER

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:ESA_02977
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeCronobacter
Proteomesi
  • UP000000260 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003669841 – 1043Beta-galactosidaseAdd BLAST1043

Proteomic databases

PRIDEiA7MN76

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi290339.ESA_02977

Structurei

3D structure databases

ProteinModelPortaliA7MN76
SMRiA7MN76
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni547 – 550Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT Bacteria
COG3250 LUCA
HOGENOMiHOG000252443
KOiK01190
OMAiDQDMFRL

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
2.70.98.10, 1 hit
HAMAPiMF_01687 Beta_gal, 1 hit
InterProiView protein in InterPro
IPR004199 B-gal_small/dom_5
IPR036156 Beta-gal/glucu_dom_sf
IPR011013 Gal_mutarotase_sf_dom
IPR008979 Galactose-bd-like_sf
IPR014718 GH-type_carb-bd
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR023933 Glyco_hydro_2_beta_Galsidase
IPR006103 Glyco_hydro_2_cat
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR032312 LacZ_4
PANTHERiPTHR10066:SF76 PTHR10066:SF76, 1 hit
PfamiView protein in Pfam
PF02929 Bgal_small_N, 1 hit
PF16353 DUF4981, 1 hit
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SMARTiView protein in SMART
SM01038 Bgal_small_N, 1 hit
SUPFAMiSSF49303 SSF49303, 2 hits
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
SSF74650 SSF74650, 1 hit
PROSITEiView protein in PROSITE
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit

Sequencei

Sequence statusi: Complete.

A7MN76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTENPTTTAF NELQTRPLAT ILARNDWQNP AITSVNRLPS HTPLHGWRDA
60 70 80 90 100
DRARRGEPSD AVLSLDGEWQ FSFFASPHQV PEVWLAADLS DARATPVPSN
110 120 130 140 150
WQMEGYDTPI YTNVRYPIPV NPPFVPDDNP TGCYSRDIEV PQAWLETGRT
160 170 180 190 200
RIIFGGVNSA FYLWCNGQWV GYSQDSRLPA EFDLTGVLHA GRNRLCVLVL
210 220 230 240 250
RWSDGTYLED QDMWRMSGIF RPVSLLHLPE QALADVRVHT ELAPSLRHAT
260 270 280 290 300
LFSEVEVTPA ACGLSVTLAL WHGENEIASQ TLPLGSAPID ERGNYAERVT
310 320 330 340 350
LSLDVDNPLL WSAETPHLYR AVVTLLDADG MPLVSEAHDV GFRRVEINNG
360 370 380 390 400
LLTLNGQPLL IRGVNRHEHH PEKGQAVDEA AMVQDILLMK QNNFNAVRCS
410 420 430 440 450
HYPNQPRWYE LCSRYGLYVV DEANIETHGM EPMSRLSDDP VWLGAYSERV
460 470 480 490 500
TRMVKCNRNH PSIIIWSLGN ESGNGATHTA LYNWIKHQDP TRPVQYEGGG
510 520 530 540 550
ADTRATDIIC PMYARVETDQ LIPAVPKWSI KKWISMPGET RPLILCEYAH
560 570 580 590 600
AMGNSLGNFA DYWAAFRQYP RLQGGFIWDW ADQAITRVEP DGSRWWAYGG
610 620 630 640 650
DFGDTPNDRQ FCMNGLVFPD RTPHPALFEA KHQQQFFQFR LVSENPLQIE
660 670 680 690 700
VTSEYLFRES DNERLLWSIE VRGETRLSGE LTLELGPQAS RVLTLSDTGF
710 720 730 740 750
SARAGDEEIW LHVRVEQPQA TPWSPEGHLS AWAQWPLAAP LALPEPVAAG
760 770 780 790 800
DAPQLEITDA EFVIRHGCQT WRVSRASGQL IQWSDDGVDQ ILTPLADQFI
810 820 830 840 850
RAPIDNDIGV SEVERIDPNA WVERWKAAGL YNTEHRCLAC DAQTTRDGVE
860 870 880 890 900
IVAQHAYFVK GVADGPAILS RWRMVVDNQG ALHCDIDIAR SAALPPLPRV
910 920 930 940 950
GVVCQLRGGE ETASWLGLGP HENYPDRLSS ACFSRWTLPL SELTTPYIFP
960 970 980 990 1000
GENGLRCNTR ELNWNGWQAE GEFHFSLSPY GTRQLMETSH WHKLQPEAGI
1010 1020 1030 1040
WLTIDGFHMG VGGDDSWTPS VHPEYLLTAR EYRYRFTLRR RQG
Length:1,043
Mass (Da):117,662
Last modified:October 2, 2007 - v1
Checksum:i269BB0FA74F2772F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA Translation: ABU78206.1
RefSeqiWP_012125561.1, NC_009778.1

Genome annotation databases

EnsemblBacteriaiABU78206; ABU78206; ESA_02977
KEGGiesa:ESA_02977
PATRICifig|290339.8.peg.2666

Similar proteinsi

Entry informationi

Entry nameiBGAL_CROS8
AccessioniPrimary (citable) accession number: A7MN76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 2, 2007
Last modified: May 23, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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