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A7MN76

- BGAL_CROS8

UniProt

A7MN76 - BGAL_CROS8

Protein

Beta-galactosidase

Gene

lacZ

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei113 – 1131SubstrateUniRule annotation
    Metal bindingi212 – 2121SodiumUniRule annotation
    Binding sitei212 – 2121SubstrateUniRule annotation
    Sitei367 – 3671Transition state stabilizerUniRule annotation
    Sitei401 – 4011Transition state stabilizerUniRule annotation
    Metal bindingi426 – 4261Magnesium 1UniRule annotation
    Metal bindingi428 – 4281Magnesium 1UniRule annotation
    Active sitei471 – 4711Proton donorUniRule annotation
    Metal bindingi471 – 4711Magnesium 1UniRule annotation
    Binding sitei471 – 4711SubstrateUniRule annotation
    Active sitei547 – 5471NucleophileUniRule annotation
    Metal bindingi607 – 6071Magnesium 2UniRule annotation
    Metal bindingi611 – 6111Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi614 – 6141SodiumUniRule annotation
    Binding sitei614 – 6141SubstrateUniRule annotation
    Binding sitei1017 – 10171SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciCSAK290339:GJ80-2967-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:ESA_02977
    OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
    Taxonomic identifieri290339 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
    ProteomesiUP000000260: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10431043Beta-galactosidasePRO_0000366984Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi290339.ESA_02977.

    Structurei

    3D structure databases

    ProteinModelPortaliA7MN76.
    SMRiA7MN76. Positions 24-1036.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni547 – 5504Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiSHLINME.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7MN76-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTENPTTTAF NELQTRPLAT ILARNDWQNP AITSVNRLPS HTPLHGWRDA     50
    DRARRGEPSD AVLSLDGEWQ FSFFASPHQV PEVWLAADLS DARATPVPSN 100
    WQMEGYDTPI YTNVRYPIPV NPPFVPDDNP TGCYSRDIEV PQAWLETGRT 150
    RIIFGGVNSA FYLWCNGQWV GYSQDSRLPA EFDLTGVLHA GRNRLCVLVL 200
    RWSDGTYLED QDMWRMSGIF RPVSLLHLPE QALADVRVHT ELAPSLRHAT 250
    LFSEVEVTPA ACGLSVTLAL WHGENEIASQ TLPLGSAPID ERGNYAERVT 300
    LSLDVDNPLL WSAETPHLYR AVVTLLDADG MPLVSEAHDV GFRRVEINNG 350
    LLTLNGQPLL IRGVNRHEHH PEKGQAVDEA AMVQDILLMK QNNFNAVRCS 400
    HYPNQPRWYE LCSRYGLYVV DEANIETHGM EPMSRLSDDP VWLGAYSERV 450
    TRMVKCNRNH PSIIIWSLGN ESGNGATHTA LYNWIKHQDP TRPVQYEGGG 500
    ADTRATDIIC PMYARVETDQ LIPAVPKWSI KKWISMPGET RPLILCEYAH 550
    AMGNSLGNFA DYWAAFRQYP RLQGGFIWDW ADQAITRVEP DGSRWWAYGG 600
    DFGDTPNDRQ FCMNGLVFPD RTPHPALFEA KHQQQFFQFR LVSENPLQIE 650
    VTSEYLFRES DNERLLWSIE VRGETRLSGE LTLELGPQAS RVLTLSDTGF 700
    SARAGDEEIW LHVRVEQPQA TPWSPEGHLS AWAQWPLAAP LALPEPVAAG 750
    DAPQLEITDA EFVIRHGCQT WRVSRASGQL IQWSDDGVDQ ILTPLADQFI 800
    RAPIDNDIGV SEVERIDPNA WVERWKAAGL YNTEHRCLAC DAQTTRDGVE 850
    IVAQHAYFVK GVADGPAILS RWRMVVDNQG ALHCDIDIAR SAALPPLPRV 900
    GVVCQLRGGE ETASWLGLGP HENYPDRLSS ACFSRWTLPL SELTTPYIFP 950
    GENGLRCNTR ELNWNGWQAE GEFHFSLSPY GTRQLMETSH WHKLQPEAGI 1000
    WLTIDGFHMG VGGDDSWTPS VHPEYLLTAR EYRYRFTLRR RQG 1043
    Length:1,043
    Mass (Da):117,662
    Last modified:October 2, 2007 - v1
    Checksum:i269BB0FA74F2772F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000783 Genomic DNA. Translation: ABU78206.1.
    RefSeqiYP_001439042.1. NC_009778.1.

    Genome annotation databases

    EnsemblBacteriaiABU78206; ABU78206; ESA_02977.
    GeneIDi5548613.
    KEGGiesa:ESA_02977.
    PATRICi20397789. VBICroSak107175_2666.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000783 Genomic DNA. Translation: ABU78206.1 .
    RefSeqi YP_001439042.1. NC_009778.1.

    3D structure databases

    ProteinModelPortali A7MN76.
    SMRi A7MN76. Positions 24-1036.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290339.ESA_02977.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABU78206 ; ABU78206 ; ESA_02977 .
    GeneIDi 5548613.
    KEGGi esa:ESA_02977.
    PATRICi 20397789. VBICroSak107175_2666.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi SHLINME.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci CSAK290339:GJ80-2967-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
      Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
      PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-894.

    Entry informationi

    Entry nameiBGAL_CROS8
    AccessioniPrimary (citable) accession number: A7MN76
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3