Reviewed, UniProtKB/Swiss-Prot A7MN74 (MAO1_ENTS8)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	ESA_02975

Organism

Enterobacter sakazakii (strain ATCC BAA-894) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	NAD-dependent malic enzyme		PRO_0000323536
Sites
	Active site	104	1	Proton donor By similarity
	Active site	175	1	Proton acceptor By similarity
	Metal binding	246	1	Divalent metal cation By similarity
	Metal binding	247	1	Divalent metal cation By similarity
	Metal binding	270	1	Divalent metal cation By similarity
	Binding site	157	1	NAD By similarity
	Binding site	270	1	NAD By similarity
	Binding site	419	1	NAD By similarity
	Site	270	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MN74-1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 12A2D79AE81592B0	FASTA	566	63,030
	10 20 30 40 50 60 MEIKHKKNRS LYIPYAGPVL LEFPLLNKGS AFSMEERSNF NLLGLLPEVV ETIEEQAERA 70 80 90 100 110 120 WRQFEDFKTD IDKHIYLRNI QDTNETLFYR LLENHLEVMM PIIYTPTVGS ACERFSEIYR 130 140 150 160 170 180 RARGVFISWP NRHNMDDILQ NVPIHNIKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 190 200 210 220 230 240 ACGGISPAYT LPIVLDVGTN NQQLLNDPLY MGWRHPRITD DEYYAFVDDF IQAVKQRWPN 250 260 270 280 290 300 VLLQFEDFAQ KNAMPLLERY RDEICCFNDD IQGTAAVTLG TLIAASRAAG SQLSEQKIVF 310 320 330 340 350 360 LGAGSAGCGI AEQIIAWMRT EGGLSDEQAR ARVFMVDRFG LLTDNMPNLL SFQSKLVQKR 370 380 390 400 410 420 DSLKGWDTQS DSISLLDVVR NAKPDILIGV SGQTGLFTEE IIREMHKHCA RPIVMPLSNP 430 440 450 460 470 480 TSRVEATPHD ILNWTDGAAL VATGSPFQPV TVKEKTYPIA QCNNAYIFPG IGLGIISSGA 490 500 510 520 530 540 LRVTDEMMMA ASEALASHSP LVNTGSGLVL PPLTDIQQVS KDIAFAVGKM AQQQGVAVKT 550 560 SAEALLQAIE ENFWLPEYRS YRRTSI

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton B., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000783 Genomic DNA. Translation: ABU78204.1. Different initiation.
RefSeq	YP_001439040.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5548577.
GenomeReviews	Gene locus ESA_02975 in contig CP000783_GR.
KEGG	esa:ESA_02975.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_ENTS8

Accession

Primary (citable) accession number: A7MN74

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	November 25, 2008
This is version 13 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)