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A7MMD8 (RNB_CROS8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exoribonuclease 2

EC=3.1.13.1
Alternative name(s):
Exoribonuclease II
Short name=RNase II
Short name=Ribonuclease II
Gene names
Name:rnb
Ordered Locus Names:ESA_01589
OrganismCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]
Taxonomic identifier290339 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter

Protein attributes

Sequence length644 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3' to 5' direction By similarity. HAMAP-Rule MF_01036

Catalytic activity

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates. HAMAP-Rule MF_01036

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01036.

Sequence similarities

Belongs to the RNR ribonuclease family. RNase II subfamily.

Contains 1 S1 motif domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandRNA-binding
   Molecular functionExonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmRNA catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

exoribonuclease II activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 644644Exoribonuclease 2 HAMAP-Rule MF_01036
PRO_1000063889

Regions

Domain561 – 64383S1 motif

Sequences

Sequence LengthMass (Da)Tools
A7MMD8 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 925299EE474D4CCB

FASTA64472,443
        10         20         30         40         50         60 
MFQDNPLLAQ LKQQLHSQTP RAEGVVKATE KGFGFLEVDA QKSYFIPPPQ MKKVMHGDRV 

        70         80         90        100        110        120 
IAVIHTEKEK ESAEPEELIE PFLTRFVGRV QKKDDRLSIV PDHPLLKDAI PCRAERGVSH 

       130        140        150        160        170        180 
DFQNGDWAVA EMRRHPLKGD RGFYAELTQF ITFGEDHFVP WWVTLARHNL EREAPDGVAT 

       190        200        210        220        230        240 
EMLDENLTRE DLTALDFVTI DSASTEDMDD ALYVEALDGD RLQLTVAIAD PTAWIAEGSK 

       250        260        270        280        290        300 
LDNIAKVRAF TNYLPGFNIP MLPRELSDDL CSLREGVQRP ALVCRMIIEA DGAISDDIHF 

       310        320        330        340        350        360 
FAAIIESKAK LAYDDVSDWL EEKGDWQPGS EAIAAQIRLL QQICQRRSAW RTEHALVFKD 

       370        380        390        400        410        420 
RPDYRFVLGE KGEVLDIVAE PRRIANRIVE ESMIAANICA ARVLRDKLGF GVYNVHAGFD 

       430        440        450        460        470        480 
PASTEQLATL LQSHGMHVDA NDVLTLPGFC KLRRELDAQP SGFLDSRIRR FQSFAEISTE 

       490        500        510        520        530        540 
PGPHFGLGLE AYATWTSPIR KYGDMVNHRL LKAIIKGESA TRPQDEATVQ MAERRRLNRM 

       550        560        570        580        590        600 
AERDVGDWLY ARFLKDKAGT DSRFAAEIID VSRGGMRVRL VDNGAVAFIP APFLHAVRDE 

       610        620        630        640 
LVCSQENGTV QIKGETVYKV TDVIDVTIAE VRMETRSVIA RPVA 

« Hide

References

[1]"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-894.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000783 Genomic DNA. Translation: ABU76843.1.
RefSeqYP_001437679.1. NC_009778.1.

3D structure databases

ProteinModelPortalA7MMD8.
SMRA7MMD8. Positions 5-644.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290339.ESA_01589.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU76843; ABU76843; ESA_01589.
GeneID5549705.
KEGGesa:ESA_01589.
PATRIC20395255. VBICroSak107175_1417.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG4776.
HOGENOMHOG000271427.
KOK01147.
OMAPTAYVAE.
OrthoDBEOG6Q5NRD.

Enzyme and pathway databases

BioCycCSAK290339:GJ80-1584-MONOMER.

Family and domain databases

HAMAPMF_01036. RNase_II.
InterProIPR011129. Cold_shock_prot.
IPR012340. NA-bd_OB-fold.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR022967. RNA-binding_domain_S1.
IPR013223. RNase_B_OB_dom.
IPR011804. RNase_II.
IPR022966. RNase_II/R_CS.
IPR004476. RNase_II/RNase_R.
[Graphical view]
PfamPF08206. OB_RNB. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTSM00357. CSP. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 4 hits.
TIGRFAMsTIGR00358. 3_prime_RNase. 1 hit.
TIGR02062. RNase_B. 1 hit.
PROSITEPS01175. RIBONUCLEASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNB_CROS8
AccessionPrimary (citable) accession number: A7MMD8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 2, 2007
Last modified: May 14, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families