A7MMD8 (RNB_CROS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exoribonuclease 2
EC=3.1.13.1

Alternative name(s):
Exoribonuclease II
Short name=RNase II
Short name=Ribonuclease II

Gene names

Name:	rnb
Ordered Locus Names:	ESA_01589

Organism

Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter › Cronobacter sakazakii

Protein attributes

Sequence length	644 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3' to 5' direction By similarity. HAMAP-Rule MF_01036
Catalytic activity	Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates. HAMAP-Rule MF_01036
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the RNR ribonuclease family. RNase II subfamily. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA catabolic process Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW exoribonuclease II activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 644	644	Exoribonuclease 2 HAMAP-Rule MF_01036		PRO_1000063889
Regions
	Domain	561 – 643	83	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	A7MMD8 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 925299EE474D4CCB	FASTA	644	72,443
	10 20 30 40 50 60 MFQDNPLLAQ LKQQLHSQTP RAEGVVKATE KGFGFLEVDA QKSYFIPPPQ MKKVMHGDRV 70 80 90 100 110 120 IAVIHTEKEK ESAEPEELIE PFLTRFVGRV QKKDDRLSIV PDHPLLKDAI PCRAERGVSH 130 140 150 160 170 180 DFQNGDWAVA EMRRHPLKGD RGFYAELTQF ITFGEDHFVP WWVTLARHNL EREAPDGVAT 190 200 210 220 230 240 EMLDENLTRE DLTALDFVTI DSASTEDMDD ALYVEALDGD RLQLTVAIAD PTAWIAEGSK 250 260 270 280 290 300 LDNIAKVRAF TNYLPGFNIP MLPRELSDDL CSLREGVQRP ALVCRMIIEA DGAISDDIHF 310 320 330 340 350 360 FAAIIESKAK LAYDDVSDWL EEKGDWQPGS EAIAAQIRLL QQICQRRSAW RTEHALVFKD 370 380 390 400 410 420 RPDYRFVLGE KGEVLDIVAE PRRIANRIVE ESMIAANICA ARVLRDKLGF GVYNVHAGFD 430 440 450 460 470 480 PASTEQLATL LQSHGMHVDA NDVLTLPGFC KLRRELDAQP SGFLDSRIRR FQSFAEISTE 490 500 510 520 530 540 PGPHFGLGLE AYATWTSPIR KYGDMVNHRL LKAIIKGESA TRPQDEATVQ MAERRRLNRM 550 560 570 580 590 600 AERDVGDWLY ARFLKDKAGT DSRFAAEIID VSRGGMRVRL VDNGAVAFIP APFLHAVRDE 610 620 630 640 LVCSQENGTV QIKGETVYKV TDVIDVTIAE VRMETRSVIA RPVA

References

[1]

"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species." Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J. PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-894.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU76843.1.
RefSeq	YP_001437679.1. NC_009778.1.
3D structure databases
ProteinModelPortal	A7MMD8.
SMR	A7MMD8. Positions 5-644.
ModBase	Search...
Protein-protein interaction databases
STRING	290339.ESA_01589.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABU76843; ABU76843; ESA_01589.
GeneID	5549705.
KEGG	esa:ESA_01589.
PATRIC	20395255. VBICroSak107175_1417.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4776.
HOGENOM	HOG000271427.
KO	K01147.
OMA	ERDIADW.
ProtClustDB	PRK05054.
Enzyme and pathway databases
BioCyc	CSAK290339:GJ80-1584-MONOMER.
Family and domain databases
HAMAP	MF_01036. RNase_II.
InterPro	IPR011129. Cold_shock_prot. IPR012340. NA-bd_OB-fold. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR022967. RNA-binding_domain_S1. IPR013223. RNase_B_OB_dom. IPR011804. RNase_II. IPR001900. RNase_II/R. IPR022966. RNase_II/R_CS. IPR004476. RNase_II/RNase_R. [Graphical view]
PANTHER	PTHR23355:SF6. PTHR23355:SF6. 1 hit.
Pfam	PF08206. OB_RNB. 1 hit. PF00773. RNB. 1 hit. PF00575. S1. 1 hit. [Graphical view]
SMART	SM00357. CSP. 1 hit. SM00955. RNB. 1 hit. SM00316. S1. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00358. 3_prime_RNase. 1 hit. TIGR02062. RNase_B. 1 hit.
PROSITE	PS01175. RIBONUCLEASE_II. 1 hit. PS50126. S1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNB_CROS8

Accession

Primary (citable) accession number: A7MMD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program